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Method for comprehensively utilizing lignocellulose biomass

A lignocellulose and biomass technology, applied in the field of comprehensive utilization of cellulose, hemicellulose and lignin in lignocellulosic biomass, can solve the problems of low enzymatic conversion rate of cellulose and low utilization rate of lignin, etc. To achieve the effect of improving the extraction rate, simplifying the production process and reducing pollution

Active Publication Date: 2014-09-03
JINAN SHENGQUAN GRP SHARE HLDG CO LTD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is to overcome the problems of low utilization rate of lignin and low enzymatic conversion rate of cellulose in the prior art when comprehensively utilizing hemicellulose, lignin and cellulose, thus proposing A kind of comprehensive utilization method of lignocellulosic biomass

Method used

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  • Method for comprehensively utilizing lignocellulose biomass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) acid hydrolysis

[0051] 10.6kg corn cobs (mass composition: moisture 6.12%, cellulose 35.19%, hemicellulose 32.1%, lignin 23.7%, other 2.95%, the same below) are broken, washed with water to remove dust, and then processed with 80kg phosphoric acid solution Hydrolysis, the mass concentration of phosphoric acid solution is 10%, the temperature of acid hydrolysis is 120 ℃, the time is 1 hour, the acid hydrolysis residue and pentose sugar solution obtained after hydrolysis are separated, wash the acid hydrolysis residue with 10kg water, cleaning solution Merge with described pentose solution, finally obtain 19.64kg acid hydrolysis residue (moisture content is about 65%, the absolute dry content of hemicellulose is 15.87%, the absolute dry content of lignin is 31.75%, the absolute dry content of cellulose 47.81%) and 80.34kg pentose solution, the concentration of pentose solution is 2.89%, and the extraction rate of hemicellulose is 68%.

[0052] The calculation formu...

Embodiment 2

[0067] (1) acid hydrolysis

[0068] Break 10.6kg of corncobs, wash with water to remove dust, and then hydrolyze with 80kg of phosphoric acid solution, the mass concentration of phosphoric acid solution is 20%, the temperature of acid hydrolysis is 100°C, and the time is 0.5 hours. After the hydrolysis is completed, the separated acid hydrolysis Residue and pentose solution, clean described acid hydrolysis residue with 10kg water, and cleaning solution merges with described pentose solution, finally obtains 19.35kg acid hydrolysis residue (moisture content is about 65%, and the dry content of hemicellulose is 15.10 %, the dry content of lignin is 31.79%, the dry content of cellulose is 48.47%) and 80.63kg pentose solution, the concentration of pentose solution is 2.96%, and the extraction rate of hemicellulose is 70%.

[0069] (2) Enzymatic hydrolysis of cellulose

[0070] Take all the acid hydrolysis residue obtained in step (1) as the cellulose substrate, and carry out cell...

Embodiment 3

[0078] (1) acid hydrolysis

[0079] Break 10.6kg of corn cobs, wash with water to remove dust, and then hydrolyze with 80kg of phosphoric acid solution, the mass concentration of phosphoric acid solution is 5%, the temperature of acid hydrolysis is 150°C, and the time is 1 hour. After the hydrolysis is completed, the separated acid hydrolysis Residue and pentose solution, wash described acid hydrolysis residue with 10kg water, and cleaning liquid merges with described pentose solution, finally obtain 20.02kg acid hydrolysis residue (water content is about 65%, and the dry content of hemicellulose is 16.05% %, the dry content of lignin is 31.5%, the dry content of cellulose is 47.97%) and 79.96kg pentose solution, the concentration of pentose solution is 2.86%, and the extraction rate of hemicellulose is 67%.

[0080] (2) Enzymatic hydrolysis of cellulose

[0081] Take the acid hydrolysis residue described in step (1) as the cellulose substrate, and carry out cellulose enzymol...

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Abstract

A method for comprehensively utilizing lignocellulose biomass comprises the following steps that: (a) the lignocellulose biomass is subjected to acid hydrolysis so as to obtain a pentose solution and acid hydrolytic residues; (b) cellulase is used for the enzymolysis of the acid hydrolytic residues, so that glucose solution and enzymatic residues are obtained, the cellulose is the cellulose which is obtained through culturing penicillium, the penicillium has a classification name of Penicilliumdecumbens PD-G3-08, is preserved in the China center for type culture collection (CCTCC) in the Wuhan university and has a preservation number of CCTCC M 2011195; (c) aqueous alkali is used to treat the enzymatic residues so as to extract lignin in the enzymatic residues and obtain alkaline hydrolytic residues; and (d) the alkaline hydrolytic residues are subjected to enzymolysis of the step (b) again or the alkaline hydrolytic residues and the new acid hydrolytic residues are merged and then are subjected to enzymolysis of the step (b) again, steps (c) and (d) are then carried out sequentially, operations are repeated, lignin is further extracted and cellulose enzymolysis is further carried out. By the method, the maximum utilization of the lignocellulose biomass resource is realized.

Description

technical field [0001] The invention relates to a method for comprehensively utilizing lignocellulosic biomass, in particular to a method for comprehensively utilizing cellulose, hemicellulose and lignin in lignocellulosic biomass. Background technique [0002] With the depletion of fossil fuel resources and the increasing environmental pollution, the use of renewable energy as a substitute for petrochemical products has become increasingly important. Fuel ethanol is the main form of biomass liquid energy and the most likely substitute for fossil fuels. At present, the world's ethanol production mainly uses starches (corn, cassava, etc.) and sugars (sugar cane, sugar beets, etc.) as raw materials for fermentation. The technology of producing ethanol by microbial fermentation is mature, but the high cost of raw materials limits the industrial application of grain fermentation to produce ethanol. At the same time, there are disadvantages such as competition with people for gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C08H7/00C13K13/00
Inventor 唐一林高绍丰张恩选韩文斌崔建丽栗昭争马军强刘洁江成真
Owner JINAN SHENGQUAN GRP SHARE HLDG CO LTD
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