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A method for separating gamma-aminobutyric acid and glutamic acid by chromatography

An aminobutyric acid and chromatographic separation technology, applied in the field of biochemical separation, can solve the problems of large loss rate of GABA, increase of GABA dissociation, increase of escape rate, etc., and achieve the effects of high selectivity, high degree of separation and simple process

Active Publication Date: 2016-05-11
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of aqueous phase system electrodialysis to remove salt and glutamic acid to refine GABA has the following defects: because the GABA aqueous solution circulates in the membrane, its escape rate will increase greatly, and the pH of the solution will increase with the removal of glutamic acid and salt. The change is large, so the dissociation of GABA in water will greatly increase, and the loss rate of GABA in the process of electrodialysis is relatively large
At present, there are still no reports on the efficient separation of γ-aminobutyric acid and glutamic acid by chromatographic technology at home and abroad. The main reason is that the special chromatographic media with significant differences in the adsorption capacity of γ-aminobutyric acid and glutamic acid are packed in the chromatographic column. It is very difficult to screen, and the selection of relevant chromatographic separation conditions is relatively difficult, and it is impossible to implement industrial applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The GABA feed solution produced by the enzymatic conversion of glutamic acid is filtered through membrane, concentrated to 20% (w / w) concentration using nanofiltration membrane, and adjusted to pH 7.5 with ammonia water; 1:1 filled calcium-type cationic chromatographic resin, controlled column temperature at 10°C, feed volume at 0.05BV, constant temperature chromatographic separation; using deionized water as eluent, controlled temperature at 10°C and flow rate at 1.0BV / h Carry out eluting under the following conditions; collect eluent and residual liquid respectively, obtain the aqueous solution of two kinds of products of decolorized GABA and glutamic acid respectively, the separation rate of two substances reaches more than 98% (w / w), and the decolorization rate of GABA reaches 96% above.

Embodiment 2

[0035] The GABA feed solution produced by enzymatic conversion of glutamic acid is filtered through the plate frame, concentrated to 80% (w / w) concentration by using heat, and adjusted to pH 8 with NaOH; according to the volume specification of the chromatographic column, the ratio of diameter to height is 1 : 30 filled lithium-type cationic chromatographic resin, control the column temperature at 40°C, the feed volume is 0.1BV, constant temperature chromatographic separation; use deionized water to adjust the pH to 8 as eluent, control the temperature at 40°C and the flow rate is 2.0BV Elution is carried out under the condition of 1 / h; the eluent and residual liquid are collected respectively to obtain the aqueous solution of two products of decolorized GABA and glutamic acid respectively, the separation rate of the two substances reaches more than 99%, and the decolorization rate of GABA reaches more than 98%.

Embodiment 3

[0037] The GABA feed solution produced by enzymatic conversion of glutamic acid is filtered through the plate frame, concentrated to 60% (w / w) concentration by using heat, and adjusted to pH 10 with KOH; according to the volume specification of the chromatographic column, the ratio of diameter to height is 1 : 40 filled with ammonia-type cationic chromatographic resin, control the column temperature at 50°C, the feed rate is 0.3BV, constant temperature chromatographic separation; use deionized water to adjust the pH to 7 as eluent, control the temperature at 50°C and the flow rate is 0.5BV Elution is carried out under the condition of / h; the eluent and residual liquid are collected respectively, and the aqueous solutions of two products of decolorized GABA and glutamic acid are respectively obtained. The separation rate of the two substances reaches more than 90%, and the decolorization rate of GABA reaches more than 81%.

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Abstract

The invention relates to a method for separating gamma-aminobutyric acid and glutamic acid by a colour spectrum. The method comprises the steps of carrying out membrane filtration, centrifugal clarification or frame filtering on a GABA (gamma-aminobutyric acid) material solution which contains glutamic acid and is converted from glutamic acid enzyme, and then preparing 1-90%(w / w) water solution by hot concentration or membrane concentration; adjusting pH of the water solution to 2-11, entering a chromatographic column filled with a special chromatography medium under the condition of 0-100 DEG C, or eluting by taking water or water which is adjusted to specific pH as an eluting agent, and respectively collecting the eluant and residual liquid, thereby obtaining two products of the GABA and glutamic acid. Efficient separation of the GABA and glutamic acid is achieved; and meanwhile, the method has decoloring effect on the GABA. Compared with the prior art, the method is simple to operate, low in cost, high in separating degree, green and environment-friendly.

Description

technical field [0001] The invention relates to an amino acid separation technology, which belongs to the field of biochemical separation, in particular to a method for separating gamma-aminobutyric acid and glutamic acid by using chromatographic technology. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA), also known as aminobutyric acid, is a naturally occurring non-protein amino acid, and its molecular formula is NH 2 CH 2 CH 2 CH 2 COOH has a relative molecular mass of 103.1, a melting point of 202°C (decomposes under rapid heating), is easily soluble in water, slightly soluble in hot ethanol, and insoluble in other organic solvents. γ-aminobutyric acid exists in animal brains and plant germs in small amounts in nature, such as about 0.1-0.3 mg / g in brain tissue, 35 mg / kg in brown rice, and 40 mg / kg in bean leaves. GABA is an important inhibitory neurotransmitter in the mammalian central nervous system, and about 50% of the central nervou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C227/40C07C229/08C07C229/24
Inventor 赵黎明赵鹤飞陈超琴刘慧渊
Owner EAST CHINA UNIV OF SCI & TECH