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Bionic process for preparing silicon oxide nano-microcapsule immobilized enzyme

A technology of nano-microcapsules and immobilized enzymes, which is applied in the direction of immobilization on or in the inorganic carrier, can solve the problem of the size of silicon oxide nano-microcapsules, and achieve mild conditions, simple preparation process, and high recovery rate of enzyme activity high effect

Active Publication Date: 2013-04-24
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report in the literature on the preparation of silicon oxide nanocapsules by biomimetic methods, and there is no report on the regulation of the size of silicon oxide nanocapsules by regulating the liposome template

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] Dissolve 0.4g of lecithin and 0.1g of cholesterol in 8mL of chloroform to form liquid a. Take 1.5mL of liquid a in a 100mL round bottom flask, and remove the solvent chloroform by rotary evaporation at 37°C until a uniform transparent film is formed on the bottle wall. Add 5mL of glucose oxidase solution (prepared with phosphate buffer solution of 0.1mol / L, pH7, the concentration is 0.2mg glucose oxidase / mL) to the above round bottom flask, ultrasonically disperse for 2h, at this time all the film on the inner wall falls off, the flask Liposome solution (18.75mg / mL, the liposome mass is calculated based on the added lecithin and cholesterol mass, and the theoretical amount of loss in the preparation process is not calculated) is obtained within the liposome solution. Take 1 mL of the above liposome solution and put it in a 2 mL centrifuge tube; shake the above sample in a vortex shaker, and slowly add 150 μL of PDADMA (15 drops / min) dropwise at the same time. Remove fr...

example 2

[0037] Dissolve 0.4g of lecithin and 0.1g of cholesterol in 8mL of chloroform to form liquid a. Take 1.5mL of liquid a in a 100mL round bottom flask, and remove the solvent chloroform by rotary evaporation at 37°C until a uniform transparent film is formed on the bottle wall. Add 5mL of glucose oxidase solution (prepared with phosphate buffer solution of 0.1mol / L, pH7, the concentration is 0.2mg glucose oxidase / mL) to the above round bottom flask, ultrasonically disperse for 2h, at this time all the film on the inner wall falls off, the flask Liposome solution (18.75mg / mL, the liposome mass is calculated based on the added lecithin and cholesterol mass, and the theoretical amount of loss in the preparation process is not calculated) is obtained within the liposome solution. Take 1 mL of the above liposome solution and put it in a 2 mL centrifuge tube; shake the above sample in a vortex shaker, and slowly add 150 μL of PDADMA (15 drops / min) dropwise at the same time. Remove fr...

example 3

[0039] Dissolve 0.4g of lecithin and 0.1g of cholesterol in 8mL of chloroform to form liquid a. Take 1.5mL of liquid a in a 100mL round bottom flask, and remove the solvent chloroform by rotary evaporation at 37°C until a uniform transparent film is formed on the bottle wall. Add 5mL of glucose oxidase solution (prepared with phosphate buffer solution of 0.1mol / L, pH7, the concentration is 0.2mg glucose oxidase / mL) to the above round bottom flask, ultrasonically disperse for 2h, at this time all the film on the inner wall falls off, the flask Liposome solution (18.75mg / mL, the liposome mass is calculated based on the added lecithin and cholesterol mass, and the theoretical amount of loss in the preparation process is not calculated) is obtained within the liposome solution. Take 1 mL of the above liposome solution and put it in a 2 mL centrifuge tube; shake the above sample in a vortex shaker, and slowly add 180 μL of PDADMA (15 d / min) dropwise at the same time, continue to sh...

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Abstract

The invention provides a bionic process for preparing silicon oxide nano-microcapsule immobilized enzyme. According to the method, enzyme is coated in liposome nano-microcapsules; with the liposome nano-microcapsules as a template, under the effect of an inducing agent poly dimethyl diallyl ammonium, tetramethyl orthosilicate hydrolysate is dehydrated on the surface of liposome, such that a silicon oxide shell layer is formed. Direct contact of enzyme and a silicon precursor is completely avoided, such that a purpose for maintaining relatively high activity of the immobilized enzyme is realized. Also, with the liposome nano-microcapsules as the template, preparation of the silicon oxide nano-microcapsules with controllable morphology can be realized. Finally, the liposome template is removed by using a low-concentration surfactant octyl polyethylene glycol phenyl ether. With the immobilized enzyme preparation method provided by the invention, an embedding rate can reach approximately 50%, such that problems of free enzyme severe wasting and immobilization cost increasing of a sol-gel method are avoided. The reaction mainly involves water phase, such that the reaction is safe and non-polluting. The preparation process is simple, and conditions are mild. An enzyme activity recovery rate is up to 71.8%.

Description

technical field [0001] The invention belongs to the field of immobilized enzymes, in particular to a biomimetic process for preparing immobilized enzymes in silicon oxide nanocapsules. Background technique [0002] The immobilized enzyme overcomes the disadvantage that the free enzyme is difficult to separate and recover, that is, after the enzyme is immobilized, it is easy to separate from the reactant, which is beneficial to the control of the entire production process. While maintaining the high-efficiency and specific catalytic activity of the enzyme, the same batch of The immobilized enzyme can be used repeatedly; it has high thermal stability, which avoids the loss of enzyme activity caused by heat treatment in some production steps; the long-term storage stability is significantly improved, and it can be stored for a long time under certain conditions. Time, so as to realize the long-term use of the enzyme in vitro; the resistance to protease is enhanced, and it is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 姜艳军朱亚男高静戴舒张炜
Owner HEBEI UNIV OF TECH
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