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Culture method of halimasch liquid bacterial strains

A technology of liquid strains and culture methods, applied in botany equipment and methods, organic fertilizers, fertilizer mixtures, etc., can solve the problems of difficult separation of medicinal components, low quality of seed bacteria, and high cost of culture medium, and improve utilization Value, stable yield of active polysaccharides, and rapid growth

Inactive Publication Date: 2013-05-01
关岭自治县丰硕食用菌农民专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The period of solid-state fermentation is too long, it is easy to be contaminated with bacteria, and it is difficult to separate the effective ingredients; at present, liquid submerged fermentation is generally popular, but the cost of the medium for fermentation culture in liquid fermentation is high, the quality of the seed bacteria is not high, and the size of the fermentation product is different. lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for cultivating liquid strains of Armillaria armillaria, the method comprising the following steps:

[0017] 1) Preparation of mother species: make test tube culture medium, 75g of corn flour, 12g of soybean flour, 13g of sucrose (white sugar), and the pH value is 6.0. And carry out autoclaving for 40 minutes, after cooling, insert a pea-sized strain block into the test tube culture medium in a sterile environment, and place it in a 25°C environment for 10 days in the dark;

[0018] 2) Shake flask culture: prepare shake flask culture medium, rice noodles 20g, sugar 15g, yeast extract 1g, peptone 5g, potassium dihydrogen phosphate 2.0g, magnesium sulfate 1g, pH value 6.5. Then crush the mother species cultivated in step 1), inoculate them into shake flasks in a sterile environment, and place the seeded shake flasks on an electromagnetic shaker at 25°C with a shaking frequency of 500 / min for 4 days ;

[0019] 3) Fermentation tank culture: Sterilize the empty ta...

Embodiment 2

[0021] A method for cultivating liquid strains of Armillaria armillaria, the method comprising the following steps:

[0022] 1) Preparation of mother species: make test tube culture medium, 60g of corn flour, 10g of soybean flour, 10g of sucrose (white sugar), pH value of 5.5. And carry out autoclaving for 30 minutes, after cooling, insert a pea-sized strain block into the test tube culture medium in a sterile environment, and place it in a 23°C environment for 6 days in the dark;

[0023] 2) Shake flask culture: prepare shake flask culture medium, rice flour 15g, white sugar 12g, yeast extract 0.5g, peptone 3g, potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.5g, pH value 5.5. Then crush the mother species cultivated in step 1), inoculate them into shake flasks in a sterile environment, and place the seeded shake flasks on an electromagnetic shaker at 26°C with a shaking frequency of 600 / min for 5 days ;

[0024] Fermentation tank culture: carry out empty tank steri...

Embodiment 3

[0026] A method for cultivating liquid strains of Armillaria armillaria, the method comprising the following steps:

[0027] 1) Preparation of mother species: make test tube culture medium, 80g of corn flour, 15g of soybean flour, 18g of sucrose (white sugar), and the pH value is 6.5. And carry out autoclaving for 45 minutes, after cooling, insert a pea-sized strain block into the test tube culture medium in a sterile environment, and place it in an environment at 28°C for 12 days in the dark;

[0028] 2) Shake flask culture: prepare shake flask medium, rice flour 25g, sugar 20g, yeast extract 2g, peptone 9g, potassium dihydrogen phosphate 2.5g, magnesium sulfate 1.25g, pH value 6.5. Then, crush the mother species cultivated in step 1), inoculate them into shake flasks in a sterile environment, and place the seeded shake flasks on an electromagnetic shaker at 28°C with a shaking frequency of 650 / min for 7 days ;

[0029] 3) Fermentation tank culture: Sterilize the empty tank...

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Abstract

The invention discloses a culture method of halimasch liquid bacterial strains. The culture method comprises the steps of parent strain preparation, shake-flask culture and fermentation tank culture. The culture method has the advantages that the raw material cost is low, the obtaining is easy, in addition, seed bacteria are subjected to fragmentation treatment, bacterial balls can become small and have the same size, the quality of the seed bacteria is improved, the growth speed of the bacterial balls is accelerated, the fermentation growth of the halimasch deep-layer liquid is fast, and the yield is greatly improved. The still standing suspension culture is adopted in the culture method, bacterial strains absorb nutrition from the culture media and absorb oxygen from air, and mycelia downwards grow until thick and strong rhizomorphs are grown and are fully filled into the whole fermentation tank. Halimasch of the obtained rhizomorphs can reach 35 to 40g through being metered by a dry weight method. Compared with the solid fermentation or the ordinary liquid deep-layer fermentation, the culture method has the advantages that the operation is simple, the separation is convenient, the period is short, the rhizomorph yield is high, and the active polysaccharide yield is also high and stable.

Description

technical field [0001] The invention belongs to the technical field of strain cultivation, in particular to a method for cultivating liquid bacterial strains of Armillaria armillaria. Background technique [0002] Armillaria mellea (Arm111aria mellea) belongs to Basidiomycotina, Agaricaceae, Agaricaceae, Armillaria genus, is a fungus that occurs in summer and autumn, can be facultative parasitism, and can be used for both medicine and food. Armillaria armillaria has anti-inflammatory, anti-tumor, anti-radiation, promoting hematopoietic function, lowering blood sugar, enhancing immunity, improving memory, etc. It can also treat common and frequently-occurring diseases such as vertigo syndrome and vascular headache caused by different causes , and has certain pharmacological and health care functions such as inhibiting tumor growth. A variety of medicines and health care products related to Armillaria have been developed, such as: Armillaria tablets, Naoxinshu oral liquid, Ar...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05F11/00C05G3/00
Inventor 肖永东
Owner 关岭自治县丰硕食用菌农民专业合作社
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