Method for preparing casein phosphopeptide

A casein phosphopeptide and casein technology, applied in the biological field, can solve problems such as high production costs, low product purity, and unguaranteed quality, and achieve the effects of mild reaction conditions, reduced production costs, and low energy consumption

Inactive Publication Date: 2013-05-22
NANJING BIOTOGETHER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The following problems mainly exist in the technological process: 1, the enzyme cannot be recycled, and the production cost is high
2. Enzyme production and enzymatic hydrolysis are not complete
3. The purity of the product is low and the quality cannot be guaranteed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 20L of pure water to the reaction kettle, start stirring at 200rpm, heat to 45°C, slowly add 2Kg of casein into the reaction kettle, adjust the pH to 7.0; put in 10g of papain. After 1 hour of enzymatic hydrolysis, the feed solution was cooled to below 30°C, ultrafiltered with a 6000 Dalton roll-type ultrafiltration membrane, concentrated to 4L, and the permeate was collected to a centrifuge collection tank. Add pure water to the concentrated solution to 16L and return to the enzymolysis tank, raise the temperature to 45°C, adjust the pH to 7.0, and continue the enzymolysis. After 1 hour of enzymatic hydrolysis, the temperature of the feed liquid was lowered to below 30°C, concentrated by ultrafiltration to 4L, and the permeate was collected into a centrifuge collection tank. Add pure water to the concentrate again to 16L and return to the enzymatic hydrolysis tank, raise the temperature to 45°C, adjust the pH to 7.0, and continue the enzymatic hydrolysis, and the e...

Embodiment 2

[0026] Add 20L of pure water to the reaction kettle, stir at 150rpm, heat to 50°C, slowly add 1Kg of casein into the reaction kettle, adjust the pH to 6.5; put in 8g of neutral protease. After 2 hours of enzymatic hydrolysis, cool the feed liquid to below 30°C, ultrafilter it with a 3000 Dalton hollow fiber ultrafiltration membrane, concentrate it to 4L, and collect the permeate to the centrifuge collection tank. Add pure water to the concentrated solution to 16L and return to the enzymolysis tank, raise the temperature to 50°C, adjust the pH to 6.5, and continue the enzymolysis. After 2 hours of enzymatic hydrolysis, the temperature of the feed solution was lowered to below 30°C, concentrated by ultrafiltration to 4L, and the permeate was collected into the centrifuge collection tank. Add pure water to the concentrate again to 16L and return to the enzymolysis tank, raise the temperature to 50°C, adjust the pH to 6.5, and continue the enzymolysis, and the enzymolysis ends aft...

Embodiment 3

[0028] Add 20L of pure water to the reaction kettle, start stirring at 300rpm, heat to 55°C, slowly add 2Kg of casein into the reaction kettle, adjust the pH to 8.0; put in 10g of compound protease. After 3 hours of enzymatic hydrolysis, the temperature of the feed liquid was lowered to below 30°C, ultrafiltration was performed with a 4000 Dalton roll-type ultrafiltration membrane, concentrated to 4L, and the permeate was collected to a centrifuge collection tank. Add pure water to the concentrated solution to 16L and return to the enzymolysis tank, raise the temperature to 55°C, adjust the pH to 8.0, and continue the enzymolysis. After 3 hours of enzymatic hydrolysis, the temperature of the feed solution was lowered to below 30°C, concentrated by ultrafiltration to 4L, and the permeate was collected into a centrifuge collection tank. Add pure water to the concentrate again to 16L and return to the enzymatic hydrolysis tank, raise the temperature to 55°C, adjust the pH to 8.0,...

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Abstract

The invention discloses a method for preparing casein phosphopeptide. The method comprises the following steps of: (1) adding protease into casein to perform enzymatic hydrolysis; (2) separating out enzymatic hydrolysate namely casein phosphopeptide by using an ultrafiltration membrane; (3) desalinating the casein phosphopeptide which is produced after ultrafiltration by using a nanofiltration membrane; and (4) adding protease into trapped fluid of the ultrafiltration membrane to perform the enzymatic hydrolysis, and repeating the steps (2) and (3). The method for preparing the casein phosphopeptide is simple to operate, low in equipment requirement, high in yield and high in product quality.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for preparing casein phosphopeptide. Background technique [0002] Casein phosphopeptides (CPPs) are natural biologically active peptides containing clustered phosphoserine, which are obtained from bovine milk casein through hydrolysis, separation and purification catalyzed by a single or multiple enzymes. It can be used as a carrier of inorganic ions. It can combine with calcium ions, ferrous ions and zinc ions at the lower end of the small intestine of animals with neutral to slightly alkaline pH to prevent the precipitation of metal ions and phosphate, and make the soluble calcium in the small intestine , iron and zinc concentrations are increased, thereby promoting the absorption and utilization of these essential metal ions. In addition, in animal and human experiments, CPPs exhibited anti-caries, anti-bone loss, enhanced fertilization and embryonic development, etc. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCY02P20/582
Inventor 陈晓春蔡家栋张磊黄小权盛兵汤亦文李淑亚
Owner NANJING BIOTOGETHER
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