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Method for extracting and purifying astilbin

A technology of astilbin and extracting solution, which is applied in the field of extracting and purifying astilbin, which can solve the problems of reduced waste liquid discharge, low yield of astilbin, waste liquid discharge, etc., to reduce the discharge of three wastes, shorten the production cycle, The effect of simplifying the production process

Inactive Publication Date: 2013-05-29
CHENGDU JIANJIANG PHARMA FACTORY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Purpose of the invention: the purpose of the present invention is to overcome the shortcomings of the prior art, such as low yield of astilbin extracted from Huangqi leaves, low product purity, complicated column chromatography treatment operation, consumption of a large amount of eluent, and discharge of a large amount of waste liquid. , to provide a new method for extracting and purifying astilbin from the leaves of Huang Qi, further improving the yield (up to 60%) and product purity (up to 95%), and the operation is simple, the amount of solvent is reduced, and the discharge of waste liquid is reduced

Method used

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  • Method for extracting and purifying astilbin

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Effect test

Embodiment 1

[0024] Take 20 kg of leaves of Huang Qi, crush them into coarse powder, put them into an extraction tank, and soak them in 80% ethanol 10 times at 20°C for 5 hours. After the extract is released, immediately add 1% activated carbon for decolorization, filter, and recover ethanol under reduced pressure to obtain the extract, add 2 times the amount of water and stir to dissolve the extract. Let it cool, let it stand for 24 hours, collect the crystals, wash with water, and vacuum-dry to obtain 632 grams of crude astilbin. Add 3160 ml of ethyl acetate, stir for 30 minutes, filter, add 500 ml of ethyl acetate to the precipitate, stir for 30 minutes, filter, and dry to obtain 545 grams of total flavonoids of Huangqi, and the extraction yield of astilbin is greater than 60%.

Embodiment 2

[0026] Add 40 grams of total flavonoids of Huangqi, add 200 milliliters of water and 20 grams of borax, heat to dissolve completely, and let it cool. Add hydrochloric acid to adjust the pH value to 6, add sodium chloride to saturation, carry out salting out, and obtain a large amount of astilbinin boric acid complex precipitation. Filter the precipitate, add 10 times of acetone for ultrasonic extraction, until the paste precipitate is evenly dispersed into crystals, and then ultrasonic for 30 minutes. The precipitate was collected by filtration, and then ultrasonically extracted with 5 times the amount of acetone for 30 minutes, while stirring continuously. Filter the precipitate, add 5 times of water to the precipitate and stir evenly, add hydrochloric acid to acidify to pH 1, leave to crystallize for 24 hours, filter the precipitate, wash with water until neutral, dry to obtain 16 grams of astilbin, add 80 ml of acetone Dissolve, add 1% activated carbon to decolorize, conce...

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Abstract

The invention discloses a method for extracting and purifying astilbin. Pure astilbin is processed by the following steps of soaking and extracting engelhardtia roxburghiana leaves serving as raw materials by using 80% ethanol, decoloring, concentrating, adding water for precipitation and filtering to collect crystals, carrying out complexation on the crystals in water, settling, salting out precipitates, extracting impurities out of the precipitates by an organic solvent, and then dissolving the precipitates, thus obtaining pure astilbin. According to the method for extracting the astilbin from the engelhardtia roxburghiana leaves, the yield is over 60% and the purity is over 95%. The method is simple and convenient, is low in cost and is suitable for industrial production; in addition, the purity of obtained astilbin is high.

Description

technical field [0001] The invention relates to a method for extracting and purifying astilbin from Chinese medicinal material Huang Qi Ye (Engelhardtia roxburghiana Wall). Background technique [0002] Astilbin (astilbin) 3,3,4', 5,7 - pentahydroxyflavanone 3- ( 6 - deoxy - (L-mannopyranside)) is a dihydroflavonol compound, which was first discovered by Kozo Hayashi and Kazahiko Ouchi in 1950 Extracted from the plant Astilbe. The structural formula of astilbin is as follows: [0003] [0004] It has been reported in the literature that astilbin has insecticidal, coenzyme A reductase, aldose reductase, analgesic and anti-edema activities. Astilbin can also significantly improve immune liver injury, and inhibit delayed hypersensitivity by inhibiting the function of activated T lymphocytes. [0005] Huang Qiye is the dried leaf of Huang Qi, a plant of the family Juglandaceae, which is distributed in Guangdong, Guangxi, Yunnan and other provinces, and the resources are qu...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 张强
Owner CHENGDU JIANJIANG PHARMA FACTORY
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