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Magnetic microsphere carrier and its making method

A technology of magnetic microspheres and carriers, applied in the field of biomaterials, can solve problems such as the reduction of reaction kinetics, and achieve the effects of regular shape, improved capacity, and mild preparation conditions

Active Publication Date: 2013-06-05
HANGZHOU JOINSTAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the solid phase carrier used is a flat substrate, the reaction between it and the molecules to be detected is completely realized through the diffusion of molecules, which makes the reaction kinetics much lower than that of microspheres.

Method used

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  • Magnetic microsphere carrier and its making method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The first step is to use magnetic polymer microspheres with a particle size of 700nm as the cue ball. There are 50wt% nano-ferric oxide particles dispersed inside the cue ball. The matrix of the magnetic microspheres is polystyrene, and the surface is polystyrene. Copolymer of ethylene and polyacrylic acid. Take 80 mg of magnetic microspheres containing carboxyl groups on the surface and disperse them into 20 mL of 10 mM MES (2-(N-morpholino)ethanesulfonic acid) solution (the solution contains 0.05 wt% Tween 20). In the three-necked flask, under stirring, add EDC / NHS activator so that the final concentration of the two is 50 mg / mL. The reaction was stirred for 15 minutes, and the unreacted EDC and NHS were washed with the help of a magnetic field to obtain activated magnetic cue balls. The activated magnetic cue balls were then dispersed into 20 mL of phosphate buffer solution (pH = 7.6, containing 0.05 wt% Tween 20). Next, add the activated magnetic cue sphere disper...

Embodiment 2

[0037] In the first step and the second step of this embodiment, except that the cue ball particle size is 5 μm carboxyl magnetic microspheres, and the daughter balls are 200 nanometer amino polystyrene microspheres, the remaining operation methods are the same as in Example 1.

[0038] In the third step, the remaining steps are the same except that 15 micrograms of assembled magnetic microspheres are added to each concentration gradient. The detection limit of HBsAg obtained through detection and calculation is 1.2 ng / mL, and the detection limit of the smooth magnetic microspheres coated with the same cue ball as the surface amine silica shell is 3.1 ng / mL.

Embodiment 3

[0040] In the first step and the second step of this embodiment, except that the cue ball particle diameter is 10 μm carboxyl magnetic microspheres, and the daughter balls are 200 nm amino polystyrene microspheres, the rest of the operation method is the same as that of Example 1.

[0041] In the third step, the remaining steps are the same except that 30 micrograms of assembled magnetic microspheres are added to each concentration gradient. The detection limit of HBsAg obtained through detection and calculation is 1.5 ng / mL, and the detection limit of the smooth magnetic microspheres coated with the same cue ball as the surface amine silica shell is 3.8 ng / mL.

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Abstract

The invention discloses a magnetic microsphere carrier and its making method. The magnetic microsphere carrier adopts a micro or submicron scale magnetic microsphere as a mother sphere, and a nanometer-scale subsphere with the surface having abundant functional groups is assembled on the surface of the mother sphere through chemical cross-linking to obtain the assembled magnetic microsphere having a structured topologic structure. The magnetic microsphere carrier can realize the efficient capture and detection of a target molecule to be detected as a carrier for identifying, capturing and controlling the target molecule to be detected. Compared with traditional smooth spherical magnetic microsphere having same surface functional groups, the magnetic microsphere having a structured topologic structure has the advantages of substantial improvement of the detection sensitivity of the in-vitro diagnosis application process, simple assembly process, structured morphology, great improvement of the microsphere surface area because of the formation of the topologic structure, and guarantee of the efficient fixing of the target biological probe molecule in the subsequent in-vitro diagnosis application process.

Description

technical field [0001] The invention relates to a biological material carrier and its preparation and application, in particular to a magnetic microsphere carrier for improving the detection sensitivity of in vitro diagnosis and its preparation method. It belongs to the technical field of biomaterials. Background technique [0002] As a solid-phase carrier for in vitro diagnostics, magnetic microspheres act as carriers for identifying, capturing, controlling and transporting target molecules to be tested. Because it is suspended in the sample to be tested during the whole reaction process, compared with the traditional porous plate carrier, the whole reaction is basically in a liquid or quasi-liquid state, and the probability of molecular collision between the carrier and the sample to be tested is greatly increased. . In addition, since the larger surface area of ​​the magnetic microspheres improves the efficiency of loading target molecules to be detected, the biological...

Claims

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Application Information

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IPC IPC(8): G01N33/553B01J13/02H01F1/42
CPCG01N33/535G01N33/553
Inventor 徐宏古宏晨王叶菲
Owner HANGZHOU JOINSTAR BIOTECH
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