Method for analyzing four benzene and toluene metabolites in urine through liquid chromatography-tandem mass spectrometry
A metabolite, tandem mass spectrometry technology, applied in analytical materials, material separation, measurement devices, etc., can solve problems such as the inability to fully reflect the exposure levels and characteristics of benzene and toluene, and the impact of efficiency, and achieve low detection limits, The effect of improving the detection rate and shortening the analysis time
Active Publication Date: 2014-04-02
NANJING QLIFE MEDICAL TESTING LAB CO LTD
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[0005] Due to the diversification of benzene and toluene metabolism pathways, and genetic differences have an impact on the efficiency of different metabolic pathways, only using trans,trans-muconic acid or phenylmercaptouric acid as biomarkers cannot fully reflect the overall status of benzene and toluene. Exposure levels and characteristics
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[0034] The urine samples of 29 cases of non-occupational exposure groups were randomly collected, and the method for analyzing four metabolites of benzene and toluene in urine by liquid chromatography tandem mass spectrometry of the present invention was used for analysis, including the following steps:
[0035] (1) Select trans, trans muconic acid, 1,2-benzenediol, phenylmercaptouric acid and N-acetyl benzyl cysteine as the characteristic metabolites of benzene and toluene; prepare the four characteristic metabolites A single standard stock solution, a single standard stock solution containing the isotopic internal standards of the above four characteristic metabolites, and a mixed standard solution containing the above four characteristic metabolites and their corresponding isotopic internal standards;
[0036] D in it 4 -t,t-MA (99.7% deuterated), 13 C 1 -1,2-Dihydroxybenzene (99% of 13 C replaced) and D 5 - S-BMA (99.1% deuterated) was purchased from C-D-N Isotope Ca...
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Abstract
The invention discloses a method for analyzing four benzene and toluene metabolites in urine through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: preparing mixed master standard operating fluid with series concentration, performing instrument analysis, and forming a standard curve according to the linear relation between the response value and the operating master standard concentration; taking a urine sample to be tested, and adding a mixed operating standard solution containing four characteristic metabolite isotope internal standard substances; adsorbing a target compound by using solid-phase extraction small column, and then performing instrument analysis; obtaining the response value of the four characteristic metabolites; and solving the concentration of the four characteristic metabolites in the urine sample according to the known coefficient f. The ultra-high pressure liquid chromatography tandem mass spectrometry method is adopted in the instrument analysis, and the chromatographic column is an inverse phase C18 chromatographic column with the particle size of less than 2mu m. According to the method, the sample pretreatment steps are simplified, the sample instrument analysis time is shortened, and the quantitative accuracy is high; and meanwhile, the instrument detection limit and the method detection limit in the method are extremely low, and the detection rate of the low-concentration non-professional exposure population is improved.
Description
technical field [0001] The invention belongs to the field of analytical chemistry, and relates to the analysis of benzene and toluene metabolites, in particular to liquid chromatography tandem mass spectrometry for analyzing four metabolites of benzene and toluene in urine (i.e., trans, transmuconic acid, 1,2-benzenedi phenol, phenylmercaptouric acid and N-acetylbenzylcysteine), the analytical method is rapid, sensitive and accurate. Background technique [0002] Benzene and toluene are widely used chemical raw materials and organic solvents. Due to their volatility, they are ubiquitous in the atmosphere and the environment. The International Cancer Research Institute (IRAC) has proved that benzene is a carcinogenic compound. Long-term exposure to benzene can cause diseases such as leukemia, aplastic anemia, and chromosomal abnormalities; although the toxicity of toluene is slightly lower than that of toluene, But it also has strong toxic and side effects on the central ner...
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IPC IPC(8): G01N30/02G01N30/06
Inventor 范瑞芳
Owner NANJING QLIFE MEDICAL TESTING LAB CO LTD