DNA (Deoxyribonucleic acid) segment and applications thereof in constructing HCV (hepatitis C virus) whole-genome expression animal model

A whole genome and fragment technology, applied in DNA/RNA fragments, recombinant DNA technology, cells modified by introducing foreign genetic material, etc., can solve the problems of not reflecting antiviral effect, immunopathological damage, etc.
CN103194445AInactive Publication Date: 2013-07-10MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
Publication Date
2013-07-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a DNA (deoxyribonucleic acid) segment and applications thereof in constructing an HCV (hepatitis C virus) whole-genome expression animal model. The nucleotide sequence of the DNA segment comprises the following sequentially series connected sequences: an operator gene sequence of bacteria tetracycline drug-resistance operon, an immediate early promoter gene sequence of human macrophage, a gene sequence of HCV whole genome, and a gene sequence of ribozyme shearing the HCV whole genome activity on DNA and / or RNA (ribonucleic acid) level. The DNA segment can be used for constructing a tetracycline or tetracycline derivative-induced HCV whole genome-expressed transgenic animal model. The immunity of the animal model is normal, thereby being capable of producing specific anti-virus immunoreaction aiming at the expression of the HCV, and further being effectively applied to the research on the immunopathogenesis damage mechanism of drug evaluation and virus infection of the HCV vaccine.
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Description

technical field

[0001] The present invention relates to a DNA fragment and its application in constructing HCV full gene expression animal model. Background technique

[0002] Hepatitis C virus (HCV) is a hepatotropic single-stranded positive-sense RNA virus with a genome size of about 9.6 kb, including an ORF encoding polyprotein and non-coding regions on both sides. At present, there are about 180 million HCV-infected patients in the world, and about 20% of them will develop into liver cirrhosis and further develop into liver cancer, thus seriously threatening human health. HCV is highly species-specific and can only infect humans and chimpanzees, but cannot naturally infect mice, thus seriously hindering the research on HCV replication and its pathogenic mechanism, as well as the development and evaluation of vaccines and antiviral drugs. The establishment of new HCV small animal models is of great significance for the study of HCV host susceptibility and replication mec...

Claims

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