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Method for detecting clenbuterol in pig hair

A detection method, the technology of clenbuterol, applied in the detection field, can solve the problems of short drug residue cycle, real-time tracking and monitoring of difficult drug residues, false positives, etc., and achieve good detection effect, good impurity removal effect, and easy operation Effect

Inactive Publication Date: 2013-07-10
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the daily detection method of clenbuterol is: collect pig urine, and use enzyme-linked immunosorbent assay or gold standard test paper method to screen, but there are also false positive problems, and the method of directly collecting pig urine detection has the problem of short drug residue cycle, which is difficult Realize real-time tracking and monitoring of drug residues

Method used

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  • Method for detecting clenbuterol in pig hair
  • Method for detecting clenbuterol in pig hair
  • Method for detecting clenbuterol in pig hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1CLE standard substance

[0026] Using water as a solution, prepare CLE standards with a content of 0.25ppm, 0.5ppm1ppm2ppm4ppm8ppm and 16ppm respectively, using a B&W TEK iRaman spectrometer, 780nm excitation light, and using instrument quantitative analysis software to make a standard curve for the concentration, such as figure 1 shown. It has a good linear relationship in the range of 0-16ppm.

Embodiment 2

[0027] Example 2 pig hair to be tested

[0028] 1. Hair digestion: Weigh 0.200g hair and add 4ml 1 molar NaOH, digest at 80°C for 10 minutes, and centrifuge at 8000 rpm for 5 minutes. Pour off the supernatant, add 1 mL of 1M NaOH solution, centrifuge at 8000 rpm for 5 minutes, and combine the supernatant for use.

[0029] 2. Liquid-liquid extraction: Add 10ml of dichloromethane to the supernatant for liquid-liquid extraction. After static layering, take the lower layer of clear liquid, and then add 5ml of 0.1M HCl to the lower layer of liquid for extraction again. After static layering, take the upper layer The supernatant was subjected to SPE (solid phase extraction.

[0030] 3. Solid-phase extraction: Take a PCX solid-phase extraction column (Tianjin Bona Agel Technology Co., Ltd.), activate it with 5mL water and 5mL methanol, pass the sample extract through the column, and use 5mL water, 5mL methanol:water at a ratio of 1:1 Wash the column with the mixed solution of the m...

Embodiment 3

[0033] Example 3 Blank pig hair (determined as blank by other standard methods)

[0034] 1. Hair digestion: Weigh 0.200g hair and add 4ml 1 molar NaOH, digest at 80°C for 10 minutes, and centrifuge at 8000 rpm for 5 minutes. Pour off the supernatant, add 1 mL of 1M NaOH solution, centrifuge at 8000 rpm for 5 minutes, and combine the supernatant for use.

[0035] 2. Liquid-liquid extraction: Add 10ml of dichloromethane to the supernatant for liquid-liquid extraction. After static layering, take the lower layer of clear liquid, and then add 5ml of 0.1M HCl to the lower layer of liquid for extraction again. After static layering, take the upper layer The supernatant was subjected to SPE (solid phase extraction.

[0036] 3. Solid-phase extraction: take PCX solid-phase extraction column, activate it with 5mL water and 5mL methanol, pass the sample extract through the column, rinse the column with 5mL water, 5mL methanol:water mixed solution of 1:1, and drain the extraction column ...

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Abstract

The invention relates to a method for detecting clenbuterol in pig hair. The detection method adopts a surface-enhanced Raman spectroscopy (SERS) and takes nano gold as an SERS substrate; the pig hair is subjected to pretreatment so as to carry out enrichment and separation on the residual clenbuterol on the hair; and an SERS spectrum of the clenbuterol is obtained through the portable Raman spectrograph. The method is simple and convenient to operate, and can be used for rapidly detecting the clenbuterol hydrochloride in the pig hair.

Description

technical field [0001] The invention relates to the detection field, in particular to a detection method of clenbuterol. Background technique [0002] Clenbuterol is a general term for a class of drugs with adrenergic receptor stimulant effects, also known as B-stimulants, and is a series of drugs that can promote the growth of lean meat and inhibit the growth of fat meat. Clenbuterol hydrochloride is currently the most One of the commonly used clenbuterol drug drugs. Clenbuterol hydrochloride (Clenbuterol, CLE), also known as ammoniacine and clenbuterol, is a white or off-white crystalline powder, odorless, bitter, melting point 161e, soluble in water, ethanol, slightly soluble in acetone, insoluble in ether. Synthesized for the first time in the United States in 1964, the main function is to relax the smooth muscle of the airway, and it is used medically to treat asthma. In 1984, American scientists accidentally discovered that adding clenbuterol hydrochloride to feed f...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28
Inventor 康怀志陈启振曾勇明刘国坤王顺木田中群
Owner XIAMEN UNIV
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