Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining turfgrass endogenous hormone

A technology of endogenous hormones and turfgrass, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation, low recovery rate, unfavorable for accurate determination of plant endogenous hormone content, etc., to achieve good repeatability, Effects of improving recovery rate and shortening sample loading time

Inactive Publication Date: 2014-12-31
BEIJING FORESTRY UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is not only complicated to operate, but also the recovery rate is quite low (about 80%), which is not conducive to the accurate determination of plant endogenous hormone content

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining turfgrass endogenous hormone
  • Method for determining turfgrass endogenous hormone
  • Method for determining turfgrass endogenous hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Select representative turfgrass (Poa annua and Lolium: separate extraction and detection, both using the method of this example) as samples. Cut 0.3g of fresh leaves, add liquid nitrogen and grind until pulverized.

[0055] (2) Add 3mL sodium phosphate buffer solution (0.05M, pH=7.0, containing 0.02% sodium diethyldithiocarbamate; keep the leaf weight g: extract volume mL=1:10), transfer to a 10mL centrifuge tube After shaking at 4°C for 1h, adjust the pH to about 2.6 with 1M hydrochloric acid, then add 3mL of dichloromethane, and shake at 4°C for 1h.

[0056] Note: Plant endogenous hormones are very unstable, so ensure that the shaking process is always maintained at 4°C.

[0057] (3) Take out the centrifuge tube, centrifuge the extract obtained in the previous step at 10,000 rpm for 10 min, remove the supernatant (about 3 mL) and transfer it to another centrifuge tube. Add 3 mL of dichloromethane to the residue, continue shaking at 4°C for 1 h, centrifuge again ...

Embodiment 2

[0079] Lawn grass is bluegrass grass, and other conditions are identical with embodiment 1, difference is:

[0080] The mass volume ratio of lawn grass to sodium phosphate buffer is 1:8 (g:mL);

[0081] The concentration of sodium diethyldithiocarbamate in the sodium phosphate buffer solution is 0.05%;

[0082] The volume ratio of phosphate buffer and dichloromethane is 3:4;

[0083] Conditions for extracting with sodium phosphate buffer: after extracting at 4°C for 0.8h, adjust the pH to 3.0 with 1M hydrochloric acid;

[0084] The conditions for the first extraction with dichloromethane and the extraction of the residue with dichloromethane: extract at 4°C for 1.2h;

[0085] The conditions for the two centrifugations include: take out the centrifuge tube, centrifuge at 8000 rpm for 15 min, remove the supernatant (about 3 mL), and transfer it to another centrifuge tube. Add 3mL of dichloromethane to the residue, continue shaking at 4°C for 1.2h, centrifuge again (15min at 8...

Embodiment 3

[0087] Lawn grass is used ryegrass, and other conditions are identical with embodiment 1, difference is:

[0088] The mass volume ratio of lawn grass to sodium phosphate buffer is 1:12 (g:mL);

[0089] Conditions for extracting with sodium phosphate buffer: after extracting for 1.2 hours at 4°C, adjust the pH to 2.5 with 1M hydrochloric acid;

[0090] The conditions for the first extraction with dichloromethane, and the extraction of the residue with dichloromethane: extraction at 4°C for 0.8h;

[0091]The conditions for the two centrifugations include: take out the centrifuge tube, centrifuge at 12000 rpm for 8 min, remove the supernatant (about 3 mL), and transfer it to another centrifuge tube. Add 3mL of dichloromethane to the residue, continue shaking at 4°C for 0.8h, centrifuge again (centrifuge at 12000rpm for 8min), and combine the supernatant;

[0092] To dissolve the eluent dried in vacuum, a mixed solution of methanol and acetic acid was used, wherein the volume ra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for determining turfgrass endogenous hormone. The method comprises the steps that: (1) turfgrass is extracted twice by using a sodium phosphate buffering solution and methylene chloride; the extract is subjected to twice centrifugation, and is blow-dried by using nitrogen gas, such that turfgrass endogenous hormone is obtained; (2) the turfgrass endogenous hormone obtained in the step (1) is dissolved by using methanol; the solution is subjected to decolorization and degreasing by using a solid-phase extraction column; an eluent is obtained, and is vacuum-dried; and the eluent is dissolved by using a mixed solution of methanol and acetic acid; and (3) methanol and acetic acid are adopted as a mobile phase, and the turfgrass endogenous hormone treated in the step (2) is determined by using high-performance liquid chromatography, such that turfgrass endogenous hormone content is obtained. According to the invention, proper endogenous hormone extraction method and chromatographic conditions are adopted, such that turfgrass endogenous hormone content is accurately determined. Compared with prior art, the method provided by the invention has the advantages of high recovery rate, high extraction rate, high accuracy, and good repeatability.

Description

technical field [0001] The invention relates to the field of determination of plant endogenous hormones, in particular to a method for determining turfgrass endogenous hormones. Background technique [0002] Since High Performance Liquid Chromatography (HPLC) was used to analyze plant endogenous hormones in the 1970s, due to its high sensitivity, selectivity, good repeatability and fast analysis speed, it has been used in all kinds of hormones except ethylene. Four categories of plant hormones and growth regulators have been continuously developed in the field of research. The focus of the quantitative determination of plant endogenous hormones by high performance liquid chromatography is to choose the appropriate extraction method and chromatographic conditions for endogenous hormones. However, due to the complexity and particularity of biological endogenous hormones, the separation and extraction of target components is an extremely critical step, and is an important fact...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/26
Inventor 常智慧禚来强尹淑霞于芳芳
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com