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Cordyceps militaris peanut and preparing method thereof

A technology of Cordyceps militaris and peanuts, which can be applied to botany equipment and methods, fertilizer mixtures, gardening, etc., and can solve problems that have not been reported before

Active Publication Date: 2014-09-10
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the biotransformation of peanuts with Cordyceps militaris, that is, the production of cordycepin in the solid fermentation of peanuts.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Peanut soaking

[0023] Weigh 250 g of Huayu No. 23 peanuts, add 500 g of water, and soak for 12 hours at room temperature to control the water content of the peanuts after soaking in the range of 100-120%.

[0024] 2) Sterilization

[0025] After the soaked peanuts are drained, they are divided into Erlenmeyer flasks, stoppered, sterilized at 121°C for 30 minutes, and cooled to be inoculated.

[0026] 3) Slant culture of Cordyceps militaris

[0027] The preserved Cordyceps militaris strain was inoculated on the slant medium and cultured at 23°C for 7-9 days. The slant medium was composed of 18-22% potato, 1-3% glucose, and peptone 0.1 ~1.0%, potassium dihydrogen phosphate 0.1~0.5%, magnesium sulfate 0.1~0.3%, vitamin B10.008~0.02%, agar 1-3%. A specific formula is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, vitamin B 10.01%, agar 2%. The preparation method is to cut 20g potatoes into pieces, add 100mL wate...

Embodiment 2

[0047] 1) Peanut soaking

[0048] Weigh 250g of Huayu No. 23 peanuts, add water in a ratio of 1:2 to 1:5, and soak for 10 to 16 hours at room temperature to control the water content of the peanuts at 100 to 120%.

[0049] 2) Peanut cellulase treatment

[0050] Drain the soaked peanuts and put them into a triangular flask. Add citric acid-disodium hydrogen phosphate buffer solution at a material-liquid ratio of 1:3 to 5, and perform cellulase treatment at 50°C for 1 to 3 hours. The enzyme content is 8000-12000U / g peanuts, rinse with running water for 10-20 minutes and then drain, and dry in the shade for 5-8 hours. Cellulase is derived from green wood enzymes with an activity of 11000 U / mg.

[0051] 3) Sterilization

[0052] Peeled peanut granules of 10-15 mesh are divided into triangular flasks, stoppered, sterilized at 121°C for 30 minutes, and then inoculated after cooling.

[0053] 4) Slant culture of Cordyceps militaris

[0054] The preserved Cordyceps militaris strain was inoculat...

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Abstract

The invention relates to a cordyceps militaris peanut which is prepared by soaking a peanut into water, sterilizing the soaked peanut, cooling the peanut, placing the peanut into cordyceps militaris seed solution for fermental cultivation, and conducting drying after fermentation to obtain the cordyceps militaris peanut. The seed coat of the peanut has proper inbibitional effects on the cordyceps militaris when the cordyceps militaris is in biological transformation in the peanut. Therefore, by peeling the soaked peanut, the content of the fermented cordyceps militaris can be improved. Further, even inoculation area is enlarged, and biological transformation amount of cordyceps taishanensis of the cordyceps militaris can be improved. Through cellulose treatment, the peeling rate of the soaked peanut is improved, and the cordyceps militaris transformation amount of the cordyceps militaris in the peanut is improved.

Description

Technical field [0001] The invention belongs to the technical field of food preparation, and specifically relates to a cordyceps militaris peanut and a preparation method thereof. Background technique [0002] Cordyceps militaris (Cordyceps militaris) is a species of Ascomycota, Sclerotium, Hypocrea, Claviceps, and Cordyceps. It is a heterogeneous fungus of the same genus as Cordyceps sinensis and is usually called Cordyceps militaris. Because it has the same medicinal and nourishing functions as Cordyceps sinensis, and is easier to cultivate than Cordyceps sinensis, Cordyceps militaris has been selected as the best substitute for Cordyceps sinensis for a large number of artificial cultivation, and it has been used as a new resource food and a new kind of medicine. Approved by the Ministry of Health and the State Food and Drug Administration to enter the market. [0003] The active ingredients of Cordyceps militaris include cordycepin, adenosine, cordyceps polysaccharide and cordy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 朴美子王莹程凡升郭芳言
Owner QINGDAO AGRI UNIV
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