Method for separating and extracting L-valine from broth with high efficiency

Abstract

The invention discloses a method for separating and extracting L-valine from broth with high efficiency, and microfiltration for the L-valine broth, ultrafiltration, condensation and crystallization are carried out in order, and an L-valine first crude product and a crude product mother liquor are obtained, and an ion exchange treatment is carried out for the crude product mother liquor, and the L-valine in the crude product mother liquor is further recycled, and after concentration and crystallization, a L-valine second crude product is prepared. The method provided by the invention has the advantages of high overall yield and prepared L-valine with high purity, and thalline proteins separated from the extraction treatment can be used as feed additives, and the eluate in the ion exchange treatment can be used as fertilizer, thereby realizing zero discharge of the ammonium-containing waste water with environment protection, and in addition reducing production cost and improving economic benefit.

Description

technical field [0001] The invention relates to the technical field of amino acid production, in particular to a method for extracting L-valine from fermentation broth. Background technique [0002] L-Valine is one of the eight essential amino acids that humans and animals cannot synthesize themselves but must rely on external sources. Because of its special structure and function, it plays a particularly important role in human life metabolism. At present, fermentation is the main method for producing L-valine. Usually L-valine fermentation broth includes valine, various metabolic by-products, culture medium residues, bacterial cells, etc. In order to obtain pure L-valine, valine must be separated from the fermentation broth and extract it. Abroad, represented by Ajinomoto, adopts the precipitation method to extract. According to the specific combination of the precipitant and valine, a precipitate is formed, and then separated and extracted. This method has the advantage...

AI Technical Summary

Problems solved by technology

The general process of this process is: the fermentation broth is filtered to remove the bacteria, and the separation and extraction of L-valine from the fermentation broth using this process has the following problems: one is to use the method of filtration to remove the bacteria, because the bacteria are small, usually the bacteria The removal is not complete; the second is that the color value of the L-valine fermentation liquid is relatively high, and the decolorization of ordinary activated carbon is not complete and the amount is large, which affects the product quality and yield; the third is that there are many impurities and high content in the L-valine fermentation liquid , direct use of ion exchange method, the resin is seriously polluted, it is easy to cause the resin structure to be broken and lose the regeneration ability, which increases the operating cost. major issues

Although this process improves the total extraction yield of the product, different grades of products are produced to meet the different needs of the market, and part of the waste water in the ion exchange process is recycled to reduce environmental pollution. However, the purity of the product is limited.

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