Rapid determination of clenbuterol in urine based on surface-enhanced Raman spectroscopy

Abstract

The invention discloses a method for rapidly detecting clenbuterol in urine based on a surface-enhanced Raman spectrum. Dichloromethane or ethyl acetate is adopted as an extraction solvent, an acidic aqueous solution and an organic solvent are adopted as a purification extracting solvent for carrying out liquid-liquid extraction on an extracting solution, nano gold or nano silver colloidal solution is adopted as an enhancing reagent, and a 785nm laser source Raman spectrum with the laser energy of 200mw is adopted for scanning a detection solution on a machine; and displacements 1258 minus or plus 2 cm<-1>, 1470 minus or plus 2 cm<-1> and 1601 minus or plus 2 cm<-1> are taken as characteristic peaks for judging clenbuterol; and normalization is carried out on strength of a corresponding peak 1450 minus or plus 2 cm<-1>, a linearity curve and the strength of the corresponding peak 1470 minus or plus 2 cm<-1> are combined to calculate content of clenbuterol, and rapid detection on clenbuterol in the urine are carried out qualitatively and quantitatively. A preparation method of a nano gold size is simple, operation time of pre-treatment is short, repeatability is good, and flexibility is high, in-field rapid detection can be realized, and supervision requirement can be met.

Description

Technical field [0001] The invention relates to a method for detecting clenbuterol in urine, in particular to a method for rapidly detecting clenbuterol in urine based on surface enhanced Raman spectroscopy. Background technique [0002] Clenbuterol is a β-adrenergic receptor agonist compound (hereinafter referred to as β-agonist), which is often used as an anti-asthmatic drug in human medicine to regulate bronchiectasis and smooth muscle Relaxation, treatment of respiratory diseases. It is called "Clenbuterol" because it can promote the growth of animals and increase the rate of lean meat. The Ministry of Agriculture Announcements No. 176 and No. 1519 clearly stipulate that the use of Clenbuterol in farmed animals is clearly prohibited, but there are still criminals who are driven by profit to illegally use Clenbuterol. Clenbuterol is the representative frequently occurring at home and abroad. "Clenbuterol" poisoning incident. In order to protect the health of the people, the...

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Problems solved by technology

[0005] There are also relevant reports about the SERS rapid detection method of beta-receptor agonists such as clenbuterol, such as Izquierdo-LorenzoI. etc. have reported utilizing SERS to detect the standard solutions of three beta-receptor agonists such as clenbuterol and albuterol , found the feasibility of the method, but the detection sensitivity was not high (the detection limit of clenbuterol was 70ng.mL -1 ), and the report did not study the SERS detection method of clenbuterol in the matrix, which could not meet the needs of actual supervision; GuichiZhu et al. used the analytical method of SERS combined with immunity to detect clenbuterol in pig urine, and the method had high sensitivity ( 1pg.mL - 1 ), but this method is based on the principle of immunology. It needs to prepare specific antibodies in the early stage, and the cycle is long. The preparation method of the enhanced reagent is relatively complicated, and the detection cycle is long

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