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Application of biocontrol fungus Trichoderma virens and metabolites thereof

A technology for metabolites and biocontrol fungi, applied in the field of microorganisms, can solve the problems of strong toxicity, high cost and high residues of chemical fungicides, and achieve the effects of strong stress resistance, fast growth rate and strong cellulose degradation ability.

Inactive Publication Date: 2013-08-14
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, environmental and safety issues caused by congenital defects such as strong toxicity, high residue, high cost and easy drug resistance of chemical fungicides have gradually become prominent in production.

Method used

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  • Application of biocontrol fungus Trichoderma virens and metabolites thereof
  • Application of biocontrol fungus Trichoderma virens and metabolites thereof
  • Application of biocontrol fungus Trichoderma virens and metabolites thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation and identification of biocontrol fungus Trichoderma virens (Trichoderma virens) SS161

[0022] Take 0.5-1 gram of rotten straw in the seedling tray (the straw comes from the experimental field of China Rice Research Institute), add 200ml of sterile water, shake at 120 rpm, 28 degrees Celsius for 30 minutes, let stand for 10 minutes, and take 100 microliters of supernatant Liquid-coated modified Martin medium plate (recipe: KH 2 PO 4 1 g, glucose 10 g, MgSO 4 ·7H 2 O 0.5 g, peptone 5.0 g, 1 / 3000 Bengal red aqueous solution 100 ml, add 1000 ml water, natural pH value, add streptomycin solution to a final concentration of 0.003% before use, add 18 g agar, sterilize), and culture in the dark at 28 °C After colony growth and unit cell separation and purification, the antagonism of each strain against various pathogenic fungi was tested by confrontation culture method, among which the antagonism of strain SS161 was the strongest, and further research ...

Embodiment 2

[0027] Embodiment 2: the activation culture of Trichoderma viridans SS161 bacterial classification

[0028]The frozen strains were inoculated on solid PDA medium, and cultured at 28°C until the mycelia covered the plate. Use a 0.5cm hole punch to take out the fungus cake for inoculation in the following scheme. The formula of solid PDA medium is: potato 200g, glucose 20g, agar 20g, distilled water 1000mL.

Embodiment 3

[0029] Embodiment 3: the preparation scheme one of Trichoderma viridans SS161 spore powder and spore suspension

[0030] Trichoderma viride SS161 was inoculated on a solid PSA plate, cultured at 28°C for 7-15 days, the spores were washed with sterile water, and the hyphae were removed by filtration to obtain a spore suspension. The spore suspension was freeze-dried to obtain Trichoderma viride SS161 spore powder. This scheme is suitable for preparing a small amount of spore powder and spore suspension for laboratory experiments. The formula of solid PSA medium is: 200g of potatoes, 20g of sucrose, 20g of agar, and 1000mL of distilled water.

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Abstract

The invention relates to application of a biocontrol fungus Trichoderma virens SS161 and metabolites thereof, belonging to the technical field of microorganisms. The collection number of the biocontrol fungus Trichoderma virens SS161 provided by the invention is CGMCC No.7224. The strain is separated from a rotten rice straw in a rice seedling breeding disc, and shows high antagonistic action on multiple pathogenetic fungi. A fermentation product of the strain has growth promoting action on rice and contains antibacterial substances. The separation of the strain provides an excellent original strain for the research and development of microorganism-derived agricultural chemicals.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of a biocontrol fungus Trichoderma viridans SS161 and its metabolites. Background technique [0002] Disease is an important factor affecting crop yield and quality, and disease prevention and control is one of the key links in agricultural production. As the vanguard of crop disease prevention and control, chemical fungicides have the advantages of economy, high efficiency, convenience, and speed, and have always been an indispensable part of the integrated crop management system, especially during the period of disease pandemic or loss of variety resistance. Rely on preventive measures. However, environmental and safety issues caused by congenital defects such as strong toxicity, high residue, high cost and easy drug resistance of chemical fungicides have gradually become prominent in production. Compared with chemical pesticides, microbial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/885
Inventor 刘连盟黄世文王玲
Owner CHINA NAT RICE RES INST
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