Ejecting-type jet flow cavitation generator and cavitation cell disruption device

A cell crushing and generator technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve problems affecting the recovery rate of active proteins, limited cavitation generator design, protein denaturation, etc. Achieve the effect of avoiding chemical oxidation, simple structure and low cost

Active Publication Date: 2013-08-14
WUHAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

The latter is suitable for relatively fragile bacteria, but repeated freezing and thawing will denature the protein, thereby affecting the recovery rate of active protein
[0007] At present, some s

Method used

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  • Ejecting-type jet flow cavitation generator and cavitation cell disruption device

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Embodiment Construction

[0031] The technical solutions of the present invention will be further specifically described below through the embodiments and in conjunction with the accompanying drawings.

[0032] This specific implementation includes a cell container 1 for storing the cell solution to be broken, a high-pressure centrifugal pump 3 connected to the cell container 1 through a pipeline, and a cavitation generator 6 connected to the outlet of the high-pressure centrifugal pump 3 through a pipeline, see figure 1 .

[0033] cavitation generator 6 see figure 2 , comprising a suction chamber 64, the nozzle is socketed in the suction chamber 64, the nozzle includes a nozzle inlet section 62 and a nozzle outlet section 63, the outlet end of the nozzle stretches into the suction chamber 64, and the high-pressure centrifugal pump 3 is connected to the inlet of the nozzle inlet section 62 through a pipeline After the suction chamber 61, the throat pipe 65, the diffusion pipe 66 and the outlet pipe 6...

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PUM

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Abstract

The invention discloses an ejecting-type jet flow cavitation generator and a cavitation cell disruption device. The cavitation generator comprises a nozzle, a suction chamber, a throat pipe, a diffuser and an outlet pipe. The nozzle is connected in the suction chamber in a sleeved manner, and a suction pipe is arranged on the side of the suction chamber. The cavitation cell disruption device comprises a container, a high-pressure centrifugal pump and the above cavitation generator, wherein the container is used for storing cells to be disrupted, the high-pressure centrifugal pump is connected with the container through a water pipe, and the cavitation generator is connected with the high-pressure centrifugal pump through the pipe. Valves are respectively arranged on an entrance of the nozzle, the suction pipe and the outlet pipe to control degree of cavitation. In the cavitation cell disruption device, high-pressure liquid sucks up low-pressure liquid to cause cavitation to happen in the tail end of the suction chamber and the throat pipe, cells are disrupted by microjet and shock waves produced from bubble collapse, and drastic shearing actions between the other two fluids can also have tearing effect to the cells. The ejecting-type jet flow cavitation generator and the cell disruption device for cavitation cells are simple in structure, low in cost, free of moving components, reliable in operating, strong in cavitation transport, high in crushing efficiency, and thereby applicable to mass application.

Description

[0001] technical field [0002] The invention belongs to the technical field of cell crushing, and in particular relates to a brand-new injection jet cavitation generator and a cavitation cell crushing device. Background technique [0003] Currently, cell disruption methods mainly include mechanical disruption, chemical and biochemical osmosis, and physical osmosis. [0004] The mechanical crushing method also includes high-pressure homogenization, bead milling, impact crushing, and ultrasonic crushing. It is characterized by large processing capacity, high crushing efficiency, and fast speed. It mainly relies on homogenization and grinding; during the process, the cell wall cannot Broken by strong mechanical shear force, resulting in cytoplasmic efflux. However, heating is the main problem in the mechanical fragmentation method, because the local high temperature generated by friction and impact will lead to the deterioration of the required substances in the cytoplasm. ...

Claims

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Application Information

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IPC IPC(8): F04F5/10C12M1/33
Inventor 龙新平肖龙洲
Owner WUHAN UNIV
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