Device and method for fixing carbon dioxide by utilizing biomineralization of spirulina platensis in seawater system
A technology of Spirulina platensis and carbon dioxide, applied in biochemical cleaning devices, biochemical equipment and methods, and methods based on microorganisms, can solve the problems of huge manpower and material resources, unstable pressure storage technology, and high cost of pressure storage technology , to achieve the effect of low cost
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Embodiment example 1
[0026] (1) Take 100mL of sterilized simulated seawater culture medium and place it in a 250mL volumetric flask, inoculate 10mL of Spirulina platensis stock solution, and put it into a light incubator for cultivation. The light intensity is 3000lux, the temperature is 35°C, and the light-to-dark ratio is 12: 12. After 5 days of culture, a small amount of inoculation was performed, and then the culture was continued under the same conditions, that is, the second expansion culture. This solution is used as the inoculation solution for subsequent experiments; take 10 ml of the second-generation expanded culture solution and inoculate it into a 5L conical flask filled with simulated seawater culture solution, and inoculate and expand the three-generation Spirulina platensis cyanobacteria according to the above method Illumination culture, when the biomass of the bacterial liquid reaches OD560nm of 0.6 or more, the bacterial liquid is connected to a 5-stage parallel continuous reacto...
Embodiment example 2
[0032] (1) Take 100mL of sterilized simulated seawater culture medium and place it in a 250mL volumetric flask, inoculate 10mL of Spirulina platensis stock solution, and put it into a light incubator for cultivation. The light intensity is 3000lux, the temperature is 35°C, and the light-to-dark ratio is 12: 12. After 5 days of culture, a small amount of inoculation was performed, and then the culture was continued under the same conditions, that is, the second expansion culture. This solution is used as the inoculation solution for subsequent experiments; take 10 ml of the second-generation expanded culture solution and inoculate it into a 5L conical flask filled with simulated seawater culture solution, and inoculate and expand the three-generation Spirulina platensis cyanobacteria according to the above method Illumination culture, when the biomass of the bacterial liquid reaches OD560nm above 0.6, the bacterial liquid is connected to a 5-stage parallel continuous reactor, an...
Embodiment example 3
[0036] (1) Take 100mL of sterilized simulated seawater culture medium and place it in a 250mL volumetric flask, inoculate 10mL of Spirulina platensis stock solution, and put it into a light incubator for cultivation. The light intensity is 3000lux, the temperature is 35°C, and the light-to-dark ratio is 12: 12. After 5 days of culture, a small amount of inoculation was performed, and then the culture was continued under the same conditions, that is, the second expansion culture. This solution is used as the inoculation solution for subsequent experiments; take 10 ml of the second-generation expanded culture solution and inoculate it into a 5L conical flask filled with simulated seawater culture solution, and inoculate and expand the three-generation Spirulina platensis cyanobacteria according to the above method Illumination culture, when the biomass of the bacterial liquid reaches OD560nm and is above 0.6, the bacterial liquid is connected to a 5-stage parallel continuous reac...
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