GC-MS/MS (Gas chromatography-tandem mass spectrometry) method for simultaneously analyzing benzo [a] BaA, chrysene and benzo [a] BaP in main stream smoke of cigarettes

Abstract

The invention discloses a GC-MS / MS (Gas chromatography-tandem mass spectrometry) method for simultaneously analyzing benzo [a] BaA, chrysene and benzo [a] BaP in main stream smoke of cigarettes. The method is characterized by comprising the following steps of: taking out a Cambridge filter disc catching granule phase objects of main stream smoke of cigarettes, adding ethyl acetate and an internal standard, carrying out vibrating extraction, carrying out dispersive solid-phase extraction (d-SPE) on extracting liquid, then, carrying out nitrogen-blowing concentration on supernatant, and measuring by using GC-MS / MS. The method provided by the invention overcomes the deficiency that the existing pretreatment is tedious. A sample pretreatment method and an instrument detection condition are optimized, aiming at the granule phase objects of main stream smoke of cigarettes; the matrix disperse solid phase extraction (d-SPE) is selected to be used as a purification measure in purifying treatment; compared with a national standard method solid phase extract phase, the operation is simple, and the price is low; and the method provided by the invention has the advantages of accurate operation, high sensitivity and good repeatability.

Description

[0001] technical field [0002] The invention belongs to the technical field of detection of harmful substances in cigarette smoke, and mainly relates to three polycyclic compounds such as benzo[a]anthracene (BaA), chrysene and benzo[a]pyrene (BaP) in cigarette mainstream smoke. Determination techniques of aromatic hydrocarbons. [0003] Background technique [0004] Benzo[a]pyrene and other polycyclic aromatic hydrocarbons are one of the main harmful substances in mainstream cigarette smoke, and have strong carcinogenic effects. The weight analysis of previous animal or human toxicology studies shows that the most carcinogenic substances in tobacco are TSNAs and PAHs have always been the hotspots of people's research. [0005] The current standard method for the determination of benzo[a]pyrene in mainstream cigarette smoke is "GB / T 21130-2007 Determination of benzo[a]pyrene in total particulate matter in cigarette smoke". The main method for analyzing BaP is GC / MS, and h...

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Problems solved by technology

[0006] GC / MS method to measure polycyclic aromatic hydrocarbons in cigarette mainstream smoke generally uses organic solvents such as n-hexane or cyclohexane containing internal standard to extract the total particle phase of smoke The extract was purified with a silica gel solid-phase extraction column. After the eluate was concentrated, it was analyzed by GC / MS and quantified by the selected ion monitoring (SIM) mode, which has high recovery rate, high sensitivity, good repeatability, and accurate quantification, etc. characteristics, but the cumbersome solid-phase extraction purification process and concentration process are required in the sample pretreatment process, which is time-consuming and laborious, and it was found in the GC / MS detection process of polycyclic aromatic hydrocarbons in the mainstream smoke of cigarettes with tar content less than 1 mg / cig, The content of benzo[a]pyrene is mostly lower than 1 ng / cig, and the GC / MS chromatographic peak of benzo[a]pyrene is low at this time, which brings certain difficulties to the quantification

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