Lophatherum gracile extract and application thereof

A technology of pale bamboo leaf and extract, applied to the pale bamboo leaf extract and its application field, can solve the problems of unclear pharmacodynamic material basis and mechanism of action, lack of specific therapeutic drugs, difficult product quality control, etc. Anti-inflammatory and antioxidant capacity, improved animal health, stable product quality

Active Publication Date: 2013-08-28
HUNAN WUJIFENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Chinese herbal medicine feed additive products are mostly Chinese medicine compound and its extract compound. For example, CN95100946.X discloses a Chinese herbal medicine preparation for preventing and treating poultry and livestock diseases. Additives and feed, and the Chinese patent application with application number 201110292217.0 discloses a natural plant feed additive and its preparation method, etc. These products generally have complex composition, unclear drug substance basis and mechanism of action, and no specific quality standards. There are defects in product quality that are difficult to control
[0003] In recent years, viral diseases have dominated animal diseases and their types have increased year by year. Viral diseases have the characteristics of rapid pathogenic mutation and lack of specific therapeutic drugs. Compared with bacterial infectious diseases, they are more difficult to diagnose and prevent.

Method used

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  • Lophatherum gracile extract and application thereof
  • Lophatherum gracile extract and application thereof
  • Lophatherum gracile extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0023] The Leaf Bamboo Extract of the present embodiment is made by the following method:

[0024] Take 100g of bamboo leaves, crush them, add 1500mL of ethanol solution with a volume concentration of 80%, reflux and extract twice in an extraction tank at 85°C for 3 hours each time, filter, and keep the filtrate and filter residue for later use; concentrate the filtrate to recover ethanol to The concentrated solution with a solid content of 12wt% was then spray-dried at 170°C to obtain the alcohol-soluble extract of Bambusa pachyrhiza; take the filtered filter residue, continue to add 1200mL of distilled water, soak and extract at 90°C for 4h, centrifuge, and concentrate the centrifugate to a solid content of 12wt%, and then spray-dried at 170° C. to obtain a water-soluble extract of Pachyphyllum leaves; mixing the resulting alcohol-soluble extracts of Pachyphyllum leaves and water-soluble extracts of Pachyphyllum leaves with a mass ratio of 1:2, Instantly.

[0025] The conte...

preparation Embodiment 2

[0028] The Leaf Bamboo Extract of the present embodiment is made by the following method:

[0029] Take 100g of bamboo leaves, crush them, add 1600mL of ethanol solution with a volume concentration of 85%, reflux and extract twice in an extraction tank at 80°C for 3 hours each time, filter, and keep the filtrate and filter residue for later use; concentrate the filtrate to recover ethanol to The concentrated solution with a solid content of 15wt% was then spray-dried at 170°C to obtain the ethanol-soluble extract of Bamboo leaf; take the filtered filter residue, continue to add 1500mL of distilled water, soak and extract at 80°C for 4h, and after centrifugation, the centrifuged liquid Concentrate to a solid content of 15wt%, and then spray-dry at 170°C to obtain a water-soluble extract of Pachyphyllum leaves; mix the resulting alcohol-soluble extracts of Pachyphyllum leaves and water-soluble extracts of Pachyphyllia leaves at a mass ratio of 1:1 , that is.

[0030] The conten...

Embodiment 3

[0032] Effects of Bamboo Leaf Extract on Disease Resistance and Antioxidative Ability of Animals

[0033]A total of 24 Du long-term three-way hybrid pigs with a similar age and a body weight of about 30 kg were selected and randomly divided into two treatment groups. The control group was fed with a basal ration, and the test group was fed with 400 g / t of the extract of the leaves of Bamboo Bamboo obtained in Example 1 in the basal ration. The basal diet was prepared according to the NRC "Pig Nutritional Requirements" (1998) to prepare a 30-60kg experimental basal diet for growing pigs. After feeding for 50 days, each treatment group randomly selected 8 test pigs with similar body weight to collect 10mL of blood from the anterior vena cava. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSP-Px) in the blood were determined.

[0034] After the serum was pre-treated, the serum immunoglobulin IgG was measured with MINDRAY BS-200 au...

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Abstract

The invention discloses a lophatherum gracile extract and application thereof. The lophatherum gracile extract is prepared by the following steps of: smashing lophatherum gracile stem leaves, adding the smashed lophatherum gracile stem leaves into an ethanol solution with the volume concentration of 70-85 percent, performing backflow extraction and filtration on the mixed solution, concentrating a filtrate until the solid content is 12-16 weight percent, performing spray drying on the concentrated filtrate to obtain lophatherum gracile alcohol-soluble extract, adding distilled water into filter residues, immersing and extracting the filter residues at 70-90 DEG C for 3-4 hours, filtering or centrifuging an extract, concentrating a filtrate or centrifugal liquid until the solid content is 12-16 weight percent, performing spray drying on the concentrated product to obtain lophatherum gracile water-soluble extract, and mixing the lophatherum gracile alcohol-soluble extract and the lophatherum gracile water-soluble extract according to a proportion of 1:(1-3) to obtain the lophatherum gracile extract. The lophatherum gracile extract is added into animal matching feeds according to the mass ratio of 0.02-0.3 weight percent. The lophatherum gracile extract is environmentally-friendly and low in cost and can improve the disease-resistant and anti-inflammatory ability as well as the health conditions of the animals.

Description

technical field [0001] The present invention relates to a light bamboo leaf extract and its application, in particular to a light bamboo leaf extract and its application in animal health care. Background technique [0002] In recent years, animal diseases have become the most important factor restricting the healthy and sustainable development of my country's aquaculture industry. Disease misdiagnosis, irrational drug use and immunization failure occur frequently in pig farms, resulting in repeated outbreaks and continuous epidemics of certain animal diseases, causing huge economic losses to farmers. At present, animal infectious diseases such as porcine blue ear disease, porcine circovirus type Ⅱ infection, swine flu, swine fever and porcine colibacillosis, etc., have caused many problems such as reduced animal production performance, low feed conversion rate, reproductive impairment and even death. This directly affects the economic benefits of the farm. For a long time,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16
Inventor 贺喜胡瑕杨强张石蕊胡国田
Owner HUNAN WUJIFENG BIOLOGICAL TECH
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