Lophatherum gracile extract and application thereof
A technology of pale bamboo leaf and extract, applied to the pale bamboo leaf extract and its application field, can solve the problems of unclear pharmacodynamic material basis and mechanism of action, lack of specific therapeutic drugs, difficult product quality control, etc. Anti-inflammatory and antioxidant capacity, improved animal health, stable product quality
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preparation Embodiment 1
[0023] The Leaf Bamboo Extract of the present embodiment is made by the following method:
[0024] Take 100g of bamboo leaves, crush them, add 1500mL of ethanol solution with a volume concentration of 80%, reflux and extract twice in an extraction tank at 85°C for 3 hours each time, filter, and keep the filtrate and filter residue for later use; concentrate the filtrate to recover ethanol to The concentrated solution with a solid content of 12wt% was then spray-dried at 170°C to obtain the alcohol-soluble extract of Bambusa pachyrhiza; take the filtered filter residue, continue to add 1200mL of distilled water, soak and extract at 90°C for 4h, centrifuge, and concentrate the centrifugate to a solid content of 12wt%, and then spray-dried at 170° C. to obtain a water-soluble extract of Pachyphyllum leaves; mixing the resulting alcohol-soluble extracts of Pachyphyllum leaves and water-soluble extracts of Pachyphyllum leaves with a mass ratio of 1:2, Instantly.
[0025] The conte...
preparation Embodiment 2
[0028] The Leaf Bamboo Extract of the present embodiment is made by the following method:
[0029] Take 100g of bamboo leaves, crush them, add 1600mL of ethanol solution with a volume concentration of 85%, reflux and extract twice in an extraction tank at 80°C for 3 hours each time, filter, and keep the filtrate and filter residue for later use; concentrate the filtrate to recover ethanol to The concentrated solution with a solid content of 15wt% was then spray-dried at 170°C to obtain the ethanol-soluble extract of Bamboo leaf; take the filtered filter residue, continue to add 1500mL of distilled water, soak and extract at 80°C for 4h, and after centrifugation, the centrifuged liquid Concentrate to a solid content of 15wt%, and then spray-dry at 170°C to obtain a water-soluble extract of Pachyphyllum leaves; mix the resulting alcohol-soluble extracts of Pachyphyllum leaves and water-soluble extracts of Pachyphyllia leaves at a mass ratio of 1:1 , that is.
[0030] The conten...
Embodiment 3
[0032] Effects of Bamboo Leaf Extract on Disease Resistance and Antioxidative Ability of Animals
[0033]A total of 24 Du long-term three-way hybrid pigs with a similar age and a body weight of about 30 kg were selected and randomly divided into two treatment groups. The control group was fed with a basal ration, and the test group was fed with 400 g / t of the extract of the leaves of Bamboo Bamboo obtained in Example 1 in the basal ration. The basal diet was prepared according to the NRC "Pig Nutritional Requirements" (1998) to prepare a 30-60kg experimental basal diet for growing pigs. After feeding for 50 days, each treatment group randomly selected 8 test pigs with similar body weight to collect 10mL of blood from the anterior vena cava. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSP-Px) in the blood were determined.
[0034] After the serum was pre-treated, the serum immunoglobulin IgG was measured with MINDRAY BS-200 au...
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