Navodon septentrionalos fish head protein antioxidant peptide as well as preparation method and application thereof

An antioxidant peptide, pufferfish technology, applied in food preparation, peptide/protein components, peptides, etc., to achieve the effect of easy monitoring, high activity, scientific and reasonable process

Active Publication Date: 2013-09-04
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The applicant found in the experiment that the preparation of antioxidant peptides by enzymatic hydrolysis technology using horse-faced fi

Method used

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  • Navodon septentrionalos fish head protein antioxidant peptide as well as preparation method and application thereof
  • Navodon septentrionalos fish head protein antioxidant peptide as well as preparation method and application thereof
  • Navodon septentrionalos fish head protein antioxidant peptide as well as preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0028] Example 1:

[0029] 1) Put the washed head of green-finned puffer into double-distilled water according to the solid-liquid ratio of 1 g: 2 mL, process it into a homogenate with a high-speed tissue masher, and then put it in according to the volume ratio of 1:5 Degrease in ether for 20 h, centrifuge at 4°C and 4500 rpm for 20 min to remove ether, collect the solids, freeze-dry to obtain defatted horse-faced puffer fish head powder;

[0030] 2) Add defatted horse-faced puffer fish head powder into phosphate buffer at a solid-to-liquid ratio of 1 g: 20 mL, and adjust the pH to 6.0 with 0.10 mol / L HCl or 0.10 mol / L NaOH to obtain a mixture;

[0031] 3) Raise the temperature of the mixture to 60°C, stir and preheat for 15 minutes, add papain according to 1.2% of the mass of the defatted horse-faced puffer fish head, enzymolysis temperature is 60°C, and enzymolysis time is 4 hours to obtain an enzymolysis solution;

[0032] 4) Warm up the obtained enzymatic hydrolyzate to 9...

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Abstract

The invention discloses a navodon septentrionalos fish head protein antioxidant peptide as well as a preparation method and an application thereof. The amino acid sequence of the antioxidant peptide is Leu-Ser-His-Gly-Pro-Tyr (LSHGPY), and molecular weight determined through ESI-MS (electrospray ionization-mass spectrometry) is 672Da. The preparation method has the advantages that a preparation technology is scientific and reasonable and an enzymolysis process can be easily monitored; and the prepared antioxidant peptide has the advantages of safety, no toxic or side effect, strong antioxidant activity and easiness in digestion and absorption and can serve as a drug, health food, a food additive and the like.

Description

technical field [0001] The invention relates to an antioxidant peptide of horse-faced fish head protein, a preparation method of the horse-faced fish head protein antioxidant peptide, and an application of the horse-faced fish head protein antioxidant peptide. Background technique [0002] Studies have shown that free radicals produced by human metabolism and food oxidative deterioration will cause serious oxidative damage to human cells and tissues, leading to aging, cancer, arteriosclerosis and cardiovascular diseases. Therefore, adding antioxidants as food additives to daily meals can help capture and neutralize free radicals, remove free radical damage to the human body and protect food from oxidative damage and deterioration. At present, the food industry generally uses chemically synthesized antioxidants, such as BHA (tert-butylhydrocarbyl anisole), BHT (2, 6-di-tert-butyl-p-cresol), and PG (propyl gallate), except for TBHQ, in To varying degrees, it has adverse effec...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/02A61K38/08A61P39/06A23L1/305
Inventor 施晓燕王斌王玉梅杨瑞婷叶吉珍
Owner ZHEJIANG OCEAN UNIV
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