Device and method for enriching denitrification-dependent anaerobic methane oxidation bacteria based on intermittent membrane bioreactor

A membrane bioreactor and reactor technology, applied in biochemical equipment and methods, biochemical cleaning devices, methods for supporting/immobilizing microorganisms, etc., can solve the problems of insufficient organic carbon sources, low C/N, etc., and achieve saving Effects of aeration energy consumption and carbon source, reduction of emissions, and simple operation and management

Active Publication Date: 2013-09-04
BEIJING UNIV OF TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention relates to a device and method for enriching denitrifying anaerobic methane oxidizing bacteria based on a batch-type membrane bioreactor, which solves the technical problem of

Method used

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  • Device and method for enriching denitrification-dependent anaerobic methane oxidation bacteria based on intermittent membrane bioreactor
  • Device and method for enriching denitrification-dependent anaerobic methane oxidation bacteria based on intermittent membrane bioreactor
  • Device and method for enriching denitrification-dependent anaerobic methane oxidation bacteria based on intermittent membrane bioreactor

Examples

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Embodiment 1

[0043] Example 1 see figure 1 As shown, a device for enriching denitrifying anaerobic methane oxidizing bacteria (N-DAMO) based on a batch membrane bioreactor (SMBR) is mainly equipped with SMBR reactor 1, and equipped with water inlet tank 2, automatic control System 3 and outlet tank 4. The water inlet tank 2 is connected to the SMBR reactor 1 through the water inlet pump 8, and the SMBR reactor 1 is provided with a hollow fiber membrane module 11 inside, and the water outlet above the upper end of the hollow fiber membrane module is connected to the outlet water pump 15 through a pipeline, and the outlet water finally enters the outlet water tank 4. The acid-base solutions in the acid buffer bottle 12 and the alkaline buffer bottle 13 are fed into the SMBR reactor 1 through the acid-base dosing peristaltic pump 14 to adjust the pH level in the SMBR reactor 1 . The methane in the methane storage tank 5 enters the SMBR reactor 1 from the aeration head 16 after the flow is a...

Embodiment 2

[0045] Example 2 see image 3 Shown, a kind of method based on the device of batch type membrane bioreactor enrichment denitrification type methane anaerobic bacteria, the steps are as follows:

[0046] Step 1, inoculate the sludge and wetland bottom sludge in the anaerobic sludge digester into the SMBR reactor (1) to perform intermittent domestication and cultivation of bacteria;

[0047] Step two, will contain NO 2 - -N but COD-free sewage is introduced into the SMBR reactor (1) and fully mixed with the sludge;

[0048] Step 3, open the methane gas flow meter (9) and argon gas flow meter (10) to feed methane and argon gas into the SMBR reactor (1), and the automatic control system (3) monitors and controls the denitrification anaerobic gas in real time. The change of pH and ORP value during the oxygen methane oxidation process, so that the pH is maintained between 7.0-8.0, and the ORP is maintained between -100mV and -400mV, and manual sampling is performed to monitor the...

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Abstract

The invention relates to a device and method for enriching denitrification-dependent anaerobic methane oxidation bacteria based on an intermittent membrane bioreactor, and belongs to the technical field of sewage treatment. The device mainly comprises an SMBR (Submerged Membrane Bioreactor), a water inlet tank, an automatic control system, a water outlet tank, a methane gas storage tank, an argon gas storage tank and the like, wherein a membrane assembly is arranged in the SMBR; and the automatic control system can be used for controlling the steps including water intake, anaerobic reaction, water discharge and sludge discharge of the SMBR. According to the device and method disclosed by the invention, a high-purity enrichment culture can be obtained by optimizing operation parameters.

Description

technical field [0001] The invention relates to an enrichment method for extremely slow-growing microorganisms, in particular to a device and method for enriching denitrifying methane anaerobic bacteria based on a batch-type membrane bioreactor, and belongs to the technical field of sewage treatment. Background technique [0002] Recent studies have found that there is a class of microorganisms that can utilize NO 2 - -N and NO 3 - -N as electron acceptor, with CH 4 Simultaneous denitrification and anaerobic methane oxidation as electron donors, the process is called denitrification anaerobic methane oxidation, such microorganisms are called N-DAMO bacteria (Nitrite-driven anaerobic methane oxidation bacteria). The reaction process that N-DAMO bacteria participate in is as follows: [0003] 5CH 4 +8NO 3 - +8H + →5CO 2 +4N 2 +14H 2 O ΔG 0' =-765kJmol -1 CH 4 (1) [0004] 3CH 4 +8NO 2 - +8H + →3CO 2 +4N 2 +10H 2 O ΔG 0' =-928kJmol -1 CH 4 (2) [00...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/12C12M1/04C12N1/20C02F3/02
CPCY02W10/10
Inventor 郭建华彭永臻王淑莹
Owner BEIJING UNIV OF TECH
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