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Magnetic cell sorting apparatus based on nanoferritin adsorption

A protein adsorption and magnetic sorting technology, applied in the field of cell sorting, can solve the problems of mixed tumor cells, high operating cost, renal function damage, etc., and achieve good sorting and elution effect, good sorting effect, and toxicity. and less side effects

Inactive Publication Date: 2013-10-02
GUANGZHOU YIDAI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The system takes about 3 hours to complete a sorting, the operating cost is high, the structure is complex, and the price is expensive (about 250,000-300,000 US dollars), the sorting purity is 60-90%, and the recovery rate of cell viability is between 60-90%. The sorted hematopoietic stem cells carry the CD34 antibody "magnetic bead cap". These magnetic bead particles are injected into the patient's body, which may cause medical safety hazards such as renal function damage.
Since FACS and MACS devices are used in autologous hematopoietic stem cell transplantation to treat patients with hematological malignancies, tumor cell contamination cannot be ruled out, and there is still a risk of tumor recurrence caused by reinfusion of residual tumor cells)

Method used

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  • Magnetic cell sorting apparatus based on nanoferritin adsorption
  • Magnetic cell sorting apparatus based on nanoferritin adsorption
  • Magnetic cell sorting apparatus based on nanoferritin adsorption

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Embodiment Construction

[0030] Such as figure 1 As shown, a cell sorting device adsorbed by a nano-iron-binding protein of the present invention comprises a sealed chamber 1 of nano-ferritin and laminar flow aseptic, and the nano-ferritin is nano-ferrous Fe2+, Fe3+ and adsorbed protein Nano-ferritin particles formed by fusion or adhesion. The adsorption protein can be non-antibody binding protein, antibody and antibody fragment, and has the function of adsorbing specific cell groups. The sealed chamber 1 is provided with a sample bottle 2 and a cell sorting tank with a magnetic field sequentially connected to the sample bottle 2 through a connecting pipe 3, a nano-iron particle eluting device, an automatic cell culture system, a recovery rinsing and filling device, A cleanliness control system that controls the temperature, humidity and air cleanliness of the airtight room, and a cell sorting monitoring system that monitors and displays the entire cell sorting process.

[0031] Such as figure 2 As...

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PUM

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Abstract

The invention belongs to the technical field of cell sorting, and concretely discloses a magnetic cell sorting apparatus based on nanoferritin adsorption. The sorting apparatus comprises nanoferritin particles and a laminar aseptic sealed chamber, and a sample bottle, and a cell sorting tank having a magnetic field, a nano-iron particle eluting device, a multi-cell automatic culturing system, a recovering, rinsing and loading device, a control system for controlling the temperature, humidity and air cleanliness in the sealed chamber, and a cell sorting monitoring system for monitoring and displaying the whole cell sorting process, which are sequentially connected with the sample bottle through a connecting tube, are arranged in the sealed chamber. The sorting apparatus has a good sorting effect, can effectively prevent the cross contamination among patients and the hybridization of tumor cells, can reach high-efficiency, high-quality and zero-pollution automatic cell sorting, is in favor of reducing the medical cost, is also convenient for the allogeneic cell sorting, is suitable for more diseases, and has wide uses.

Description

technical field [0001] The invention belongs to the technical field of cell sorting. It particularly relates to a cell magnetic sorting device adsorbed by nanometer iron-binding protein. Background technique [0002] High-dose chemoradiotherapy significantly improves the curative effect of malignant tumors (such as leukemia, malignant lymphoma and multiple malignant melanoma, etc.), but also severely inhibits the hematopoietic and immune functions of tumor patients. Nearly 30 years of clinical research around the world have proved that hematopoietic stem cell transplantation and immune cell therapy are effective methods for rebuilding the hematopoietic and immune capabilities of patients with leukemia after chemotherapy. [0003] Cell sorting technology is the key to cell therapy and is widely used in hospitals and research centers. There are two methods of cell transplantation: allogeneic cell transplantation and autologous cell transplantation. Compared with allogeneic c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/38C12M1/36C12M1/34
Inventor 汪华
Owner GUANGZHOU YIDAI PHARMA
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