Bai yang dian virus and duck plague virus double fluorescent quantitation RT-PCR detection reagent kit

Abstract

The invention discloses a bai yang dian virus and duck plague virus double fluorescent quantitation RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection reagent kit which comprises a primer group and a probe group, wherein the primer group comprises a primer 1, a primer 2, a primer 3 and a primer 4; the primer 1, the primer 2, the primer 3 and the primer 4 have base sequences of SEQ. ID. No. (Sequence Identifier Number) 1, SEQ. ID. No. 2, SEQ. ID. No. 3 and SEQ. ID. No. 4 respectively; the probe group comprises a probe A and a probe B; and the probe A and the probe B have base sequences of SEQ. ID. No. 5 and SEQ. ID. No. 6 respectively. An experiment proves that the reagent kit has the advantages of short detection time, high detection sensibility and high specificity, can detect and identify a bai yang dian virus and a duck plague virus simultaneously, and can quantify the content of corresponding pathogeny of the bai yang dian virus and the duck plague virus, so that the reagent kit can be used for evaluation of curative effects of bai yang dian virus and / or duck plague virus vaccine and drug, and for research of pathogenesis and the like, and has important significance in prevention and cure of the bai yang dian virus and the duck plague virus.

Description

technical field [0001] The invention belongs to the technical field of RT-PCR detection kits, in particular to a double fluorescent quantitative RT-PCR detection kit for duck yellow virus and duck plague virus. Background technique [0002] Bai yang dian virus (BYD), also known as duck Tembusu virus, mainly causes a sharp loss of appetite, a sharp drop in egg production, follicle degeneration, and theca hemorrhage in breeding ducks and laying ducks. Since 2010, Fujian, Zhejiang, Jiangsu, Shandong, Anhui, Henan, Hebei, Guangdong and other places in China have successively reported an outbreak of a disease that caused a sharp decline in duck egg production, causing great economic losses to the laying duck farming industry. Duck plague virus (DPV) is a herpes virus that causes acute septicemia infectious diseases of ducks, geese and other waterfowl. All can be infected, but Muscovy ducks, Shelduck ducks, and Sheep ducks are the most susceptible. In natural epidemics, adult duc...

AI Technical Summary

Problems solved by technology

However, it is much more complicated to establish a multiplex fluorescent PCR method than a single-plex one, and it has higher requirements on reagents and primers. At the same time, it is necessary to ensure that there is no mutual interference between the fluorophores labeled by different probes. The fluorescent quantitative PCR instrument used with corresponding multiple detection channels

At present, there is no report on the detection and diagnosis of duck yellow virus and duck plague virus using multiplex fluorescent quantitative PCR technology

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