Biological selenium product applied to organic selenium-rich agriculture and preparation method thereof

A biological selenium and agricultural product processing technology, applied in the biological field, can solve problems such as non-compliance, doubtful safety of selenium-enriched products, environmental pollution, etc., and achieve the effects of ensuring supply, avoiding environmental pollution risks, and high biomass

Inactive Publication Date: 2013-10-09
湖南角山米业有限责任公司
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selenium sources used in selenium-enriched agricultural production without exception are inorganic selenium compounds, which makes consumers doubt the safety of selenium-enriched products, does not meet the requi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1:

[0021] Screening process for high-efficiency strains of transformed selenium Candida utilis: starting bacteria activation culture → ultraviolet mutagenesis treatment → chemical mutagenesis treatment → primary screening of resistant plates → re-screening of shake flask culture → strains with high selenium transformation ability. The implementation steps are as follows:

[0022] 1) Dilute the cultured Candida utilis culture cells in a shake flask for 24 hours with sterile saline, place them in 6 petri dishes, and irradiate them with 50Lx ultraviolet light for 10 to 120 seconds in a dark room.

[0023] 2) Add 6 test tubes containing 0.2% diethyl sulfate solution to the bacterial cells after ultraviolet irradiation and shake for 15 minutes, terminate the reaction with 2% sodium thiosulfate solution, centrifuge, and wash with sterile physiological saline.

[0024] 3) Prepare a plate medium with glucose 3%, peptone 1%, yeast extract 0.1%, agar 2%, and selenium concentrat...

Example Embodiment

[0035] Example 2:

[0036] Screening process of high-efficiency strains of transformed selenium Candida utilis: starting bacteria activation culture → chemical mutagenesis treatment → ultraviolet mutagenesis treatment → primary screening of resistant plates → re-screening of shake flask culture → strains with high selenium transformation ability. The implementation steps are as follows:

[0037] 1) Take the 24 hours of Candida utilis culture in a shake flask and add them to 6 test tubes containing 2.0% diethyl sulfate solution and shake for 5 minutes. The reaction is terminated by 2% sodium thiosulfate solution. Centrifuge and wash with sterile saline.

[0038] 2) Transfer the bacterial cells treated with diethyl sulfate into a petri dish, dilute with sterile normal saline, and irradiate with 50Lx ultraviolet light for 10 to 120 seconds in a dark room.

[0039] 3) Prepare a plate medium with glucose 3%, peptone 1%, yeast extract 0.1%, agar 2%, and selenium concentration 100~300mg / L, ...

Example Embodiment

[0050] Example 3:

[0051] Screening process for high-efficiency strains of transformed selenium Candida utilis: starting bacteria activation culture → ultraviolet mutagenesis treatment → chemical mutagenesis treatment → primary screening of resistant plates → re-screening of shake flask culture → strains with high selenium transformation ability. The implementation steps are as follows:

[0052] 1) Dilute the cultured Candida utilis culture cells in a shake flask for 24 hours with sterile saline, place them in 6 petri dishes, and irradiate them with 100Lx ultraviolet light for 10 to 120 seconds in a dark room.

[0053] 2) Add 6 test tubes containing 0.2% diethyl sulfate solution to the bacterial cells after ultraviolet radiation and shake for 15 minutes, stop the reaction with 2% sodium thiosulfate solution, centrifuge, and wash with sterile physiological saline.

[0054] 3) Prepare a plate medium with glucose 3%, peptone 1%, yeast extract 0.1%, agar 2%, and selenium concentration 10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the biotechnology field, and relates to a biological selenium product applied to organic selenium-rich agriculture and a preparation method thereof. According to the method, by-products, such as molasses and bean pulp, from processing of agriculture products are taken as a fermentation substrate; an inorganic selenium compound is added into the fermentation substrate and is transformed into biological selenium though the fermentation by transformed selenium produced muscle candida efficient strains cultivated through induced mutation; the non-poisonous biological selenium product, which is easy to be absorbed by plants, is prepared by enzymolysis of selenium macromolecules. All the selenium derives from the process of biosynthesis and transformation. When the product is applied to the production of selenium-rich organic agricultural products, not only is the safety of the selenium-rich products guaranteed, but also the risk of environment pollution caused by utilizing the inorganic selenium compound in production is effectively avoided.

Description

Technical field [0001] The present invention belongs to the field of biotechnology and relates to agricultural products added with inorganic selenium The by-product is the substrate, through the selection of Candida utilis with high selenium conversion ability to ferment and transform inorganic selenium, and prepare biological selenium products that can be easily absorbed and utilized by plants through enzymatic hydrolysis and their production technology. Background technique [0002] Selenium is an essential trace element for humans and animals. Existing studies have shown that there are hundreds of diseases in humans and animals associated with insufficient selenium intake. The endemic disease, Keshan disease, which occurs in northern China, is caused by selenium deficiency. Food is the main source of human and animal intake of selenium. Because selenium is in the crust The degree of selenium is low and unevenly distributed, and regions with varying degrees of selenium defici...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N13/00C12N15/01C12P9/00C12R1/72
Inventor 吴永尧
Owner 湖南角山米业有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products