Biological selenium product applied to organic selenium-rich agriculture and preparation method thereof
A biological selenium and agricultural product processing technology, applied in the biological field, can solve problems such as non-compliance, doubtful safety of selenium-enriched products, environmental pollution, etc., and achieve the effects of ensuring supply, avoiding environmental pollution risks, and high biomass
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Embodiment 1
[0021] Screening process for high-efficiency strains of transformed selenium Candida utilis: starting bacteria activation culture → ultraviolet mutagenesis treatment → chemical mutagenesis treatment → primary screening of resistant plates → re-screening of shake flask culture → strains with high selenium transformation ability. The implementation steps are as follows:
[0022] 1) Dilute the cultured Candida utilis culture cells in a shake flask for 24 hours with sterile saline, place them in 6 petri dishes, and irradiate them with 50Lx ultraviolet light for 10 to 120 seconds in a dark room.
[0023] 2) Add 6 test tubes containing 0.2% diethyl sulfate solution to the bacterial cells after ultraviolet irradiation and shake for 15 minutes, terminate the reaction with 2% sodium thiosulfate solution, centrifuge, and wash with sterile physiological saline.
[0024] 3) Prepare a plate medium with glucose 3%, peptone 1%, yeast extract 0.1%, agar 2%, and selenium concentration 100~300mg / L, an...
Embodiment 2
[0036] Screening process of high-efficiency strains of transformed selenium Candida utilis: starting bacteria activation culture → chemical mutagenesis treatment → ultraviolet mutagenesis treatment → primary screening of resistant plates → re-screening of shake flask culture → strains with high selenium transformation ability. The implementation steps are as follows:
[0037] 1) Take the 24 hours of Candida utilis culture in a shake flask and add them to 6 test tubes containing 2.0% diethyl sulfate solution and shake for 5 minutes. The reaction is terminated by 2% sodium thiosulfate solution. Centrifuge and wash with sterile saline.
[0038] 2) Transfer the bacterial cells treated with diethyl sulfate into a petri dish, dilute with sterile normal saline, and irradiate with 50Lx ultraviolet light for 10 to 120 seconds in a dark room.
[0039] 3) Prepare a plate medium with glucose 3%, peptone 1%, yeast extract 0.1%, agar 2%, and selenium concentration 100~300mg / L, and the bacteria af...
Embodiment 3
[0051] Screening process for high-efficiency strains of transformed selenium Candida utilis: starting bacteria activation culture → ultraviolet mutagenesis treatment → chemical mutagenesis treatment → primary screening of resistant plates → re-screening of shake flask culture → strains with high selenium transformation ability. The implementation steps are as follows:
[0052] 1) Dilute the cultured Candida utilis culture cells in a shake flask for 24 hours with sterile saline, place them in 6 petri dishes, and irradiate them with 100Lx ultraviolet light for 10 to 120 seconds in a dark room.
[0053] 2) Add 6 test tubes containing 0.2% diethyl sulfate solution to the bacterial cells after ultraviolet radiation and shake for 15 minutes, stop the reaction with 2% sodium thiosulfate solution, centrifuge, and wash with sterile physiological saline.
[0054] 3) Prepare a plate medium with glucose 3%, peptone 1%, yeast extract 0.1%, agar 2%, and selenium concentration 100~300mg / L, and the ...
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