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Method for detecting polyaromatic hydrocarbons (PAHs) and phthalic acid esters (PAEs) in plant extract

A technology of phthalates and plant extracts, applied in the field of detection of polycyclic aromatic hydrocarbons and phthalates, can solve the problem of inability to simultaneously detect toxic and harmful substances of polycyclic aromatic hydrocarbons and phthalates low detection limit, easy operation and good repeatability

Inactive Publication Date: 2013-11-13
陕西出入境检验检疫局检验检疫技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a detection method for polycyclic aromatic hydrocarbons and phthalates in plant extracts, which solves the problem that existing detection methods cannot simultaneously detect the two major categories of polycyclic aromatic hydrocarbons and phthalates. Hazardous matter

Method used

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  • Method for detecting polyaromatic hydrocarbons (PAHs) and phthalic acid esters (PAEs) in plant extract
  • Method for detecting polyaromatic hydrocarbons (PAHs) and phthalic acid esters (PAEs) in plant extract
  • Method for detecting polyaromatic hydrocarbons (PAHs) and phthalic acid esters (PAEs) in plant extract

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Weigh 1.000g of commercially available green tea extract into a 50mL glass centrifuge tube, add 4mL of water and 4mL of organic solvent in sequence, the organic solvent is n-hexane, vortex and mix well, ultrasonically extract for 25 minutes, and centrifuge at 2000 for 3 minutes. Take the supernatant, add it to a glass centrifuge tube containing 0.1 g of ethylenediamine-N-propylsilane (PSA), vortex for 1 min, and centrifuge at 2000 rpm for 3 min.

[0043] Chromatographic conditions: DB-5MS (30m×0.25mm×0.25μm), carrier gas: helium (purity ≥99.999%), flow rate 1.0mL / min, inlet temperature 280°C, mass spectrometer transfer line temperature 290°C, injection volume 1.0 μL, sampling method: splitless injection, open the split valve after 1.0 min. Programmed temperature rise: the initial temperature is 80°C, keep for 1min, rise to 225°C at 12°C / min, then rise to 265°C at 6°C / min, and finally rise to 290°C at 5°C / min, keep for 8min. Mass spectrometry conditions: electron bombar...

Embodiment 2

[0046] Still taking the above-mentioned commercially available green tea extract as the object, weigh 1.000g into a 50mL glass centrifuge tube, add 5mL of water and 5mL of organic solvent in sequence. Centrifuge for 3 minutes. Take the supernatant, add it to a glass centrifuge tube containing 0.1 g of ethylenediamine-N-propylsilane (PSA), vortex for 2 minutes, and centrifuge at 2500 rpm for 5 minutes.

[0047] Chromatographic conditions: DB-5MS (30m×0.25mm×0.25μm), carrier gas: helium (purity ≥99.999%), flow rate 1.0mL / min, inlet temperature 280°C, mass spectrometer transfer line temperature 290°C, injection volume 1.0 μL, sampling method: splitless injection, open the split valve after 1.0 min. Programmed temperature rise: the initial temperature is 80°C, keep for 1min, rise to 225°C at 12°C / min, then rise to 265°C at 6°C / min, and finally rise to 290°C at 5°C / min, keep for 8min. Mass spectrometry conditions: electron bombardment ion source (EI); ionization energy 70eV; ion ...

Embodiment 3

[0050] Still taking the above-mentioned commercially available green tea extract as the object, weigh 1.000g into a 50mL glass centrifuge tube, add 6mL of water and 6mL of organic solvent in sequence. Centrifuge for 3 minutes. Take the supernatant, add it to a glass centrifuge tube containing 0.1 g of ethylenediamine-N-propylsilane (PSA), vortex for 2 minutes, and centrifuge at 2500 rpm for 5 minutes.

[0051] Chromatographic conditions: DB-5MS (30m×0.25mm×0.25μm), carrier gas: helium (purity ≥99.999%), flow rate 1.0mL / min, inlet temperature 280°C, mass spectrometer transfer line temperature 290°C, injection volume 1.0 μL, sampling method: splitless injection, open the split valve after 1.0 min. Programmed temperature rise: the initial temperature is 80°C, keep for 1min, rise to 225°C at 12°C / min, then rise to 265°C at 6°C / min, and finally rise to 290°C at 5°C / min, keep for 8min. Mass spectrometry conditions: electron bombardment ion source (EI); ionization energy 70eV; ion ...

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Abstract

The invention discloses a method for detecting polyaromatic hydrocarbon and phthalate in a plant extract. The method comprises the following steps of: firstly pretreating the plant extract and secondly detecting polyaromatic hydrocarbon and phthalate in the plant extract through a gas chromatography-tandem mass spectrometry method. The method is simple and convenient to operate, has the advantages of low detection limit and good repeatability, and is applicable to detection of PAHs and PAEs in plant extracts.

Description

technical field [0001] The invention belongs to the technical field of detection of harmful substances, and relates to a detection method of harmful substances, in particular to a detection method of polycyclic aromatic hydrocarbons and phthalates in plant extracts. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of hydrocarbons composed of two or more benzene rings. Their chemical structures are stable, difficult to degrade, and have teratogenic, carcinogenic, and mutagenic effects. PAHs are mainly produced by the incomplete combustion of carbon-containing organic matter such as coal, petroleum, and wood. Due to their semi-volatility, they can migrate with air pollutants, easily settle on plant surfaces, and enter plant extracts along with production middle. Phthalates, also known as plasticizers, are commonly used plasticizers (phthalate esters, PAEs), which can increase the flexibility and plasticity of products, and are widely used in p...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 孔祥虹何强王宏邹阳
Owner 陕西出入境检验检疫局检验检疫技术中心
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