Method for preparing rebaudioside M through enzyme method
A technology for enzymatic preparation and rebaudioside, which is applied in the field of biological preparation of rebaudioside M, can solve problems such as no commercial production of rebaudioside M, and achieves shortening production cycle, expanding application scope, and producing The effect of cycle shortening
Active Publication Date: 2013-11-20
PEPSICO INC
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The invention relates to a method for preparing rebaudioside M through an enzyme method. According to the method, rebaudioside A or rebaudioside D is used as a substrate, and the substrate reacts to generate the rebaudioside M in the presence of a glucosyl donor under the catalytic action of UDP-glucosyltransferase and/or recombinant cells containing the UDP-glucosyltransferase. The method for preparing rebaudioside M through an enzyme method has important application value; and compared with the existing technology of extracting rebaudioside M from stevia rebaudian leaves, the method provided by the invention obviously shortens the production cycle, improves the productivity and lowers the cost, and can provide products having higher purity. Thus, the method can be used in the food and beverage industry in a more economical manner.
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Technology Topic
Enzyme methodUdp glucosyltransferase +5
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