A kind of method for the preparation of Yunzhi glycopeptide

A technology of Yunzhi glycopeptide and mycelium, applied in the biological field, can solve the problems of low yield of Yunzhi glycopeptide, hinder product development, low economic benefits, etc., and achieve the improvement of production technology and industrialization level, easy separation, The effect of high decolorization efficiency

Active Publication Date: 2017-10-13
中科医药行业生产力促进中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the process of producing Yunzhi glycopeptide by Yunzhi fermentation, there are problems such as large investment, high cost, low yield of Yunzhi glycopeptide, and low economic benefits, which hinder the development of products.

Method used

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  • A kind of method for the preparation of Yunzhi glycopeptide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] After the microbial fermentation is completed, 50L of the fermented liquid is separated by centrifugation, and the supernatant is reserved for later use; the mycelium is dried, ground, and accurately weighed, and 0.3mol / L NaOH lye is added at a ratio of 1:10, and extracted twice at 90°C , once for 2 hours, combine the extracts for subsequent use; collect the mycelia residues of alkali extraction, dry them, grind them finely, weigh them accurately, add 0.5mol / L NaOH lye in a ratio of 1:10, and extract twice at normal temperature. One time for 2 hours, the extracts are combined for later use, the residue after alkali extraction is added with 0.3mol / L NaOH lye in a ratio of 1:10, extracted twice at 90°C, one time for 2 hours, mycelium extract and mycelium residue extract Combined, concentrated under reduced pressure to about 1 / 5 of the original volume, after adding HCl to neutralize, the obtained neutralized extract was combined with the supernatant obtained after fermentat...

Embodiment 2

[0024] After the microbial fermentation is completed, 50L of the fermented liquid is separated by centrifugation, and the supernatant is reserved for later use; the mycelium is dried, ground, and accurately weighed, and 0.5mol / L of NaHCO is added at a ratio of 1:11 3 Alkaline solution, extract twice at 92°C, once for 2 hours, combine the extracts for later use; collect the mycelia residue of alkali extraction and dry, grind finely, accurately weigh, add 0.5mol / L NaOH alkali in a ratio of 1:11 solution, extracted twice at room temperature, once for 2 hours, the extracts were combined for later use, the residue after alkali extraction was added with 0.5mol / L NaOH lye at a ratio of 1:10, extracted twice at 92°C, once for 2 hours, mycelium was extracted combined with the mycelium residue extract, concentrated under reduced pressure to about 1 / 5 of the original volume, neutralized by adding HCl, and combined with the supernatant obtained after fermentation and centrifugation, and co...

Embodiment 3

[0026] After the microbial fermentation is completed, 50L of the fermentation liquid is separated by centrifugation, and the supernatant is reserved for use; the mycelium is dried, ground, and accurately weighed, and 0.6mol / L of NaHCO is added at a ratio of 1:10 3 Alkaline solution, extract 3 times at 90°C, 2 hours each time, combine the extracts for later use; collect the mycelia residue of alkali extraction for drying, grind finely, accurately weigh, add 0.8mol / L NaHCO at a ratio of 1:10 3 Alkaline solution, extract twice at room temperature, once for 2 hours, combine the extracts for later use, add 0.6mol / L NaHCO to the residue after alkali extraction at a ratio of 1:10 3 Alkaline solution, extract twice at 92°C, once for 2 hours, combine mycelium extract with mycelium residue extract, concentrate under reduced pressure to about 1 / 5 of the original volume, add HCl for neutralization, and obtain a neutralized extract Combine with the supernatant obtained after fermentation a...

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Abstract

The invention discloses a preparation method of Yunzhi glycopeptide, which belongs to the category of biotechnology. The present invention not only extracts the extracellular glycopeptides in the fermentation broth by the method of water extraction and alcohol precipitation, but also processes the mycelia through the method of alkali extraction to obtain intracellular glycopeptide substances, and regenerates the mycelium residue after alkali extraction. Extraction with cold alkali and hot alkali in turn increases the yield of intracellular glycopeptides. The total yield of Yunzhi glycopeptides can reach more than 10.50g / L, and the sugar content in the obtained Yunzhi glycopeptides reaches more than 56.00%. The content reaches more than 35.80%. The invention has the advantages of advanced technology, simple process, high yield, low cost, high product purity, etc., and is beneficial to the improvement of production technology and industrialization level of versicolor glycopeptide in my country.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for efficiently preparing versicolor glycopeptides. 2. Background technology [0002] Versicolor [Coriolus versicolor], also known as Melaleuca versicolor, Versicolor versicolor, Multicolor versicolor versicolor, Coriolus versicolor, etc., belongs to Mycology, Basidiomycotina, Polyporaceae ( Polyporales), Trametes (Trametes). Previous name: Polystictuis Versicolor(L)Fr. [0003] Polysacchride Peptide of Coriolus versicolor (PSP for short) is a kind of proteoglycan extracted from some genera and species in the Chinese Versicolor versicolor Basidiomycetes Polyporaceae. It is a good biological effect agent and is suitable for It is used in the treatment of esophageal cancer, gastric cancer and other cancers. It has immunoregulatory effect, has a certain protective effect on the cellular immune function and blood system of tumor patients, and can improve the quality of life o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/375C07K1/36C07K1/30C07K1/34
Inventor 王福清王福华殷红
Owner 中科医药行业生产力促进中心有限公司
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