Gamma-aminobutyric acid black tea wine and preparation method thereof
A technology of aminobutyric acid black tea and aminobutyric acid, which is applied in the field of food processing, can solve the problems of environmental pollution, low added value, unsuitability for drinking, etc., and achieves the effects of simple method and short production cycle
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Embodiment 1
[0034] The preparation method of the banana extract is as follows: take a certain amount of banana peel and crush it, add deionized water according to the ratio of the fresh weight of the banana peel to the water weight volume ratio of 1:2, extract at room temperature for 2 hours, centrifuge to get the supernatant, Get banana peel extract. Define the amount of enzyme required to catalyze the decarboxylation of glutamic acid to produce 1umol of GABA per minute as one enzyme activity unit, and the enzyme activity of supernatant GAD is 1.8U / mL;
[0035] Take 50 grams of crushed red tea powder, add 2000ml of deionized water, and extract in a boiling water bath for 10 minutes. After cooling, add 1500ml of banana peel extract, adjust the pH to 5.5-6.0, enrich for 0.5h under stirring, and the GABA content in the solution reaches 0.39mg / mL; saccharomyces yeast with a mass volume ratio of 10%, fermented for 2 days at 30°C and a shaker speed of 160rpm, and centrifuged to obtain black t...
Embodiment 2
[0037] The preparation method of the banana extract is as follows: take a certain amount of banana peel and crush it, add deionized water according to the ratio of the fresh weight of the banana peel to the water weight volume ratio of 1:2, extract at room temperature for 2 hours, centrifuge to get the supernatant, Get banana peel extract. Define the amount of enzyme required to catalyze the decarboxylation of glutamic acid to produce 1umol of GABA per minute as one enzyme activity unit, and the enzyme activity of supernatant GAD is 1.8U / mL;
[0038] Take 50 grams of crushed red tea powder, add 2000ml of deionized water, and extract in a boiling water bath for 10 minutes. After cooling, add 1500ml of banana peel extract, adjust the pH to 5.5-6.0, enrich for 2 hours under stirring, and the GABA content in the solution reaches 0.43mg / mL; Yeast, sucrose with a mass volume ratio of 10%, fermented for 3 days at 30°C and a shaker speed of 160rpm, centrifuged to obtain black tea fru...
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