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Cadmium adsorbent adopting engineering saccharomyces cerevisiae living cells displaying recombinant protein on the cell surfaces

A technology of Saccharomyces cerevisiae and yeast cells, applied in genetic engineering, recombinant DNA technology, fermentation and other directions, can solve the problems of difficult control of ferrite method, lack of cadmium specificity, secondary pollution, etc., and achieve good selective removal effect. Effect

Active Publication Date: 2015-06-17
惠州大亚湾聚联信息科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutralization precipitation method and sulfide precipitation method are simple in process, but easy to cause secondary pollution
The ferrite method is not easy to control and is difficult to be widely used in industry
[0004] The above methods all have the disadvantage of high cost when treating low-concentration cadmium-containing wastewater, and lack of specificity for cadmium, making it difficult to selectively recover cadmium

Method used

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  • Cadmium adsorbent adopting engineering saccharomyces cerevisiae living cells displaying recombinant protein on the cell surfaces
  • Cadmium adsorbent adopting engineering saccharomyces cerevisiae living cells displaying recombinant protein on the cell surfaces
  • Cadmium adsorbent adopting engineering saccharomyces cerevisiae living cells displaying recombinant protein on the cell surfaces

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] The preparation method of this adsorbent is:

[0012] 1. Design of T68CadR protein

[0013] Since the N-terminal of the CadR protein is a DNA-binding domain, and the 21 amino acids at the C-terminal do not perform the function of cadmium binding, these amino acids are redundant sequences for the cadmium adsorption function, so the present invention removes 47 amino acids at the N-terminal of the CadR protein. amino acids and 21 amino acids at the C-terminal, thus designing the T68CadR protein.

[0014] 2. Plasmid transformation

[0015] Using oligonucleotides TN47-F and TC21-R as primers and Pseudomonas putida genomic DNA as a template to carry out polymerase chain reaction (PCR), amplified with 5' and 3' ends respectively EcoR I and not The T68CadR coding gene of I restriction enzyme cutting site, this gene fragment is cloned into vector pYD1, obtains plasmid pYD1-T68CadR ( figure 1 ).

[0016] 3. Transformation of Saccharomyces cerevisiae

[...

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Abstract

The invention provides a cadmium adsorbent adopting engineering saccharomyces cerevisiae living cells displaying a T68CadR recombinant protein on the cell surfaces. The T68CadR recombinant protein is prepared by removing 47 amino acids at the N end and 21 amino acids at the C end of a wild CadR protein, and displaying on the surfaces of the engineering saccharomyces cerevisiae cells trough an alpha-lectin saccharomyces cerevisiae cell surface displaying system. The cadmium adsorbent has god removing effects for low-concentration cadmium in water, and has certain specificity to the cadmium.

Description

technical field [0001] The invention relates to a cadmium adsorbent for live cells of Saccharomyces cerevisiae, in particular to a cadmium adsorbent for live cells of Saccharomyces cerevisiae whose surface displays T68CadR recombinant protein. Background technique [0002] Cadmium is a highly toxic heavy metal, which forms cumulative poisoning after entering the human body, leading to osteoporosis and kidney failure. Cadmium and cadmium-containing compounds are mainly used in industries such as batteries, electroplating, pigments and alloys. With the development of industry, cadmium pollution is becoming more and more serious. [0003] At present, the methods except for cadmium-containing wastewater are mainly physical and chemical methods and chemical methods. Among them, physical and chemical methods include membrane separation method and ion exchange method. Although these methods have good removal effects, they are expensive and difficult to apply on a large scale. Che...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/22C12N1/19C12N15/31C12N15/63C12R1/865
Inventor 李鹏松陶虎春苏捷于太安彭智文
Owner 惠州大亚湾聚联信息科技有限公司