Lactobacillus rhamnosus and application thereof
A technology of Lactobacillus rhamnosus and rhamnose, which is applied in the field of microorganisms, can solve the problems that there are no relevant reports on the inhibition and enhancement of immunity of Lactobacillus rhamnosus, and achieve enhanced immunity, strong antibacterial ability, and improved lymphatic The effect of cell transformation
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Embodiment 1
[0016] Example 1 Screening and identification of Lactobacillus rhamnosus
[0017] Collect the middle and late part of fresh stool of healthy adults, mash the stool with a sterile glass rod, add 10 times the weight of PBS buffer, shake vigorously for 30 minutes, centrifuge at 2000g for 5 minutes, and collect the supernatant; centrifuge the supernatant at 5000g for 10 minutes to collect Precipitate; Wash the precipitate twice with PBS buffer; add a small amount of PBS buffer to fully suspend the precipitate; spread the suspension on MRS selective medium supplemented with calcium carbonate and culture it anaerobicly at 37°C for 48 hours; select large colonies and fast growth The strains were further streaked and purified.
[0018] The isolated strain was named YJM-L01. The colonies are round or round, with neat edges, the colony size is generally 2~3mm, milky white. The bacterium was preliminarily identified as Lactobacillus rhamnosus from the physiological and biochemical identific...
Embodiment 2
[0022] Example 2: Screened Lactobacillus rhamnosus YJM-L01 acid and bile salt tolerance experiment
[0023] 2.1 Acid resistance test
[0024] Adjust the pH value of the MRS liquid medium to 2.0, 3.0, 4.0; sterilize at 121°C for 15 minutes; inoculate the activated Lactobacillus rhamnosus into the cooled medium according to the inoculation amount of 2%, and place it at 37°C constant temperature Incubate in an incubator, shake well every 30 minutes, take samples at 0h, 1h, and 2h, and count the plates to determine the number of viable bacteria. The results are shown in Table 1.
[0025] Table 2: Acid resistance test of Lactobacillus rhamnosus
[0026]
[0027] It can be seen from Table 2 that the Lactobacillus rhamnosus selected by the present invention is cultured in MRS medium with pH 2.0 for 1 hour, and the viable rate is as high as 80%, indicating that the strain has strong acid tolerance and can be effective Resistance to gastric juice shows that the Lactobacillus rhamnosus of the ...
Embodiment 3
[0037] Example 3 Experiment of Lactobacillus rhamnosus inhibiting Escherichia coli 0157:H7
[0038] The Lactobacillus rhamnosus of the present invention was inoculated into MRS liquid medium, and after 18 hours of culture, the OD was measured by 500 Adjust the value of viable bacteria to about 5×10 8 cfu / mL. At the same time, inoculate Escherichia coli in nutrient broth, culture at 37°C for 18 hours, adjust the number of viable bacteria to 1×10 6 cfu / mL. When the nutrient agar medium is cooled to about 40℃, pour it into a sterile petri dish. After it has solidified, draw 1mL of Escherichia coli bacteria liquid and spread it evenly on the surface of the plate. Fix and cultivate the colony at 37℃ for 30min, and pour a 4mm diameter plate on the plate. Hole, the hole depth is 3mm. Pour the adjusted concentration of the bacterial solution to the full hole, incubate at 37°C in a microaerobic environment for 48 hours to observe the results, and determine the size of the inhibition zone...
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