Method for promoting seed germination of cercidiphyllum japonicum and improving germination ratio of seeds
A germination rate and seed technology, which is applied in the field of breaking the dormancy of the seeds of Lianxiang tree, promoting germination and increasing the germination rate of seeds, can solve the problems of limiting the propagation of Lianxiang tree and the low efficiency of seed reproduction, and achieve the improvement of the internal enzymes of the seeds. The effect of activity, reduction of restriction, germination vigor and germination increase
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Embodiment 1
[0020] A kind of method that promotes the seed germination of Lianxiang tree and improves seed germination rate, specifically as follows:
[0021] First, select suitable local seeds free of diseases and insect pests, and select the newly collected seeds from which the particles are plump and shiny for later use; then wash the seeds twice with warm water, and then put them in 0.5% potassium permanganate solution for 2 soaks. hour, then use mass fraction as 5% hydrogen peroxide for 3 hours, then filter and remove the disinfectant; then wash the seeds 2 times with sterile water to obtain sterilized seeds;
[0022] After drying the sterilized seeds naturally, irradiate with He-Ne laser biological irradiation instrument for 4-10min at night; wherein the He-Ne laser biological irradiation instrument is produced by Nanjing Laser Instrument Factory, the wavelength is 632.8nm, and the power density is 5mW / mm 2 , the diameter of the spot is 1.5mm (this operation does not involve cell f...
Embodiment 2
[0027] A method for promoting seed germination and improving seed germination rate of Lianxiang tree, the specific operations are as follows:
[0028] First, select suitable local seeds free of diseases and insect pests, and select the newly collected seeds from which the particles are plump and shiny for later use; then the seeds are washed with warm water for 3 times and then soaked in 1% potassium permanganate solution for 1 hour. Then use 10% hydrogen peroxide to treat for 2 hours, then filter and remove the disinfectant; then wash the seeds with sterile water for 3 times to obtain sterilized seeds;
[0029] After drying the sterilized seeds naturally, irradiate with He-Ne laser biological irradiation instrument for 4-10min at night; wherein the He-Ne laser biological irradiation instrument is produced by Nanjing Laser Instrument Factory, the wavelength is 632.8nm, and the power density is 5mW / mm 2 , the diameter of the spot is 1.5mm (this operation does not involve cell ...
Embodiment 3
[0034] A kind of method that promotes the seed germination of Lianxiang tree and improves seed germination rate, specifically as follows:
[0035] First, select suitable local seeds without pests and diseases, and select the newly collected seeds from which the full and shiny ones are for use; then the seeds are washed 3 times with warm water and then soaked in 0.8% potassium permanganate solution for 1.5 hours. Treat with 8% hydrogen peroxide for 2.5 hours, then filter and remove the disinfectant; then wash the seeds with sterile water 3 times to obtain sterilized seeds;
[0036] After drying the sterilized seeds naturally, irradiate with He-Ne laser biological irradiation instrument for 4-10min at night; wherein the He-Ne laser biological irradiation instrument is produced by Nanjing Laser Instrument Factory, the wavelength is 632.8nm, and the power density is 5mW / mm 2 , the diameter of the spot is 1.5mm (this operation does not involve cell fission, mutation, strong repeat...
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