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Preparation method and application of effective components of polygonum capitatum

An effective component, the technology of Polygonum cephalosporin, which is applied in the field of preparation of effective components of Polygonum cephalosa, can solve the problems of unfavorable efficacy of Polygonum cephalosa and lack of quality control methods, and achieve the effect of improving drug efficacy and efficiency

Active Publication Date: 2013-12-11
GUIYANG MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from our research, this method is not aimed at removing gallic acid and tannin components, and there is no relevant quality control means. The obtained components contain tannin compounds and more than 2% of gallic acid and its components. Analogues are not conducive to the efficacy of Polygonum cephalosa, and the preparation process of the effective components needs to be further optimized

Method used

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  • Preparation method and application of effective components of polygonum capitatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Pulverize 2 kg of Polygonum cephalosporin, decoct twice with 10 times the amount of water, each decocting time is 2 hours, combine the two extracts to obtain the water extract;

[0032] Put the water extract on the D101 macroporous adsorption resin chromatography column and elute with water until the water eluent FeCl 3 The reaction was negative (TLC (chloroform / methanol / formic acid=9 / 1 / 0.1), gallic acid components and FeCl 3 blue-black). Collect the water eluate, add 60% ethanol by weight, precipitate the polysaccharide and protein in the water eluate, concentrate and dry to obtain gallic acid components.

[0033] After the water elution is completed, the remaining part of the D101 macroporous adsorption resin chromatography column is eluted with 80% by weight ethanol until the reaction of hydrochloric acid-magnesium powder is negative (flavonoids react with hydrochloric acid-magnesium powder to appear red), and collect Eluent I.

[0034] Put the eluent I on the nyl...

Embodiment 2

[0037] Pulverize 2 kg of Polygonum cephalosporin, decoct 3 times with 12 times the amount of water, each decocting time is 1.5 hours, combine the three extracts to obtain the water extract;

[0038] Put the water extract on the D201 macroporous adsorption resin chromatography column and elute with water until the water eluent FeCl 3 The reaction was negative (TLC (chloroform / methanol / formic acid=9 / 1 / 0.1), gallic acid components and FeCl 3 blue-black).

[0039] After the water elution is completed, the remaining part of the D201 macroporous adsorption resin chromatography column is eluted with 70% ethanol by weight until the reaction of hydrochloric acid-magnesium powder is negative (flavonoids react with hydrochloric acid-magnesium powder to appear red), and collect Eluent I.

[0040] Put the eluent I on a nylon 66 polyamide column, recover the effluent, and then elute with 95% by weight ethanol until the reaction of hydrochloric acid-magnesium powder is negative (the reacti...

Embodiment 3

[0042]Pulverize 2 kg of Polygonum cephalosporin, decoct 3 times with 8 times the amount of water, each decocting time is 2 hours, combine the three extracts to obtain the water extract;

[0043] After the water extract is applied to the AB-8 macroporous adsorption resin chromatography column, it is eluted with water until the water eluent FeCl 3 The reaction was negative (TLC (chloroform / methanol / formic acid=9 / 1 / 0.1), gallic acid components and FeCl 3 blue-black).

[0044] After the water elution is completed, the remaining part of the AB-8 macroporous adsorption resin chromatography column is eluted with 95% by weight ethanol until the reaction of hydrochloric acid-magnesium powder is negative (the reaction of flavonoids with hydrochloric acid-magnesium powder is red) , collect the eluate I.

[0045] Put the eluent I on a nylon 11 polyamide chromatography column, recover the effluent, and then elute with 60% by weight ethanol until the reaction of hydrochloric acid-magnesiu...

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Abstract

The invention relates to a preparation method and application of effective components of polygonum capitatum, and belongs to the field of extraction of effective components of traditional Chinese medicines. The preparation method comprises the following steps: a, taking polygonum capitatum medicinal raw material, adding water for extraction, so as to obtain water extracting solution; b, processing the water extracting solution by using a macroporous adsorption resin chromatographic column; c, firstly eluting the sampled macroporous adsorption resin chromatographic column by using water, then eluting by using 70-95wt% ethanol, and collecting eluent I; d, processing the eluent I by a polyamide chromatographic column; e, recovering effluent liquid from the sampled polyamide chromatographic column, eluting by using 40-95wt% ethanol, collecting eluent II; f, mixing the effluent liquid and the eluent II, recovering ethanol, concentrating and drying to finally obtain the effective components of polygonum capitatum. The preparation method has the beneficial effect that the effective components of the polygonum capitatum, which has strong antibacterial effect and can be used for preparing anti-infection drugs can be prepared by reasonable extracting and separating methods.

Description

technical field [0001] The invention belongs to the field of extraction of effective components of traditional Chinese medicines, and in particular relates to a preparation method and application of effective components of Polygonum cephalum. Background technique [0002] Polygonum capitatum (Polygonum capitatum), also known as Shimangcao, Four Seasons Red, Red Sour Stalk, and Provincial Butterfly, belongs to the perennial herb of the Polygonaceae (Polygonaceae) genus (Polygonum). It is mainly produced in Guizhou and is an authentic medicinal material in Guizhou. Contained in the 2003 edition of "Quality Standards of Traditional Chinese Medicine and Ethnic Medicine of Guizhou Province", it has the effects of clearing away heat and detoxifying, dispelling blood stasis, diuresis and treating stranguria. Inflammation, cystitis, and pyelonephritis have good curative effect and high safety in clinical use (Review of Miao medicine-Polygonum cephalosporin. Evaluation and Analysis o...

Claims

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Application Information

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IPC IPC(8): A61K36/704A61P31/04
CPCY02A50/30
Inventor 廖尚高王永林关焕玉刘俊宏李勇军孙佳王正兰燕宇王爱民郑林黄勇
Owner GUIYANG MEDICAL UNIVERSITY
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