Wheat germ protein source antioxidative peptide, and preparation method and application thereof

Abstract

The invention relates to a wheat germ protein source antioxidative peptide, and a preparation method and an application thereof. The wheat germ protein source antioxidative peptide comprises sequences shown as SEQ ID NO.1 and SEQ ID NO.2. The preparation method comprises the steps of making fresh wheat germs into defatted wheat germ protein powder; hydrolysizing with an alkaline protease, inactivating enzyme, centrifuging at a high speed, subjecting supernatant to ultrafiltration so as to remove large molecular substances, freeze-drying filtrate to obtain a wheat germ protein hydrolysate, passing a wheat germ protein hydrolysate solution through a macroporous absorbent resin column and a gel filtration chromatography column successively, and collecting components with the strongest antioxidant activity being a target product. The preparation method is simple and efficient in process. The obtained wheat germ protein source antioxidative peptide has relatively high antioxidant activity, has relatively small molecular weight and definite amino acid sequence, can clear free radicals and alleviate oxidative stress injuries, can also be used for antioxidant dietary supplements, and has the advantages of wide raw material sources, high security, low cost and the like, and has wide application prospects in the fields of foods, medicines, etc.

Description

technical field [0001] The invention particularly relates to an antioxidant peptide derived from wheat germ protein, a preparation method and application thereof, and belongs to the technical field of intensive processing of wheat by-products. Background technique [0002] Wheat germ is a by-product of wheat processing, and its yield is increasing with the improvement of milling technology. At present, wheat germ is widely used to extract wheat germ oil. However, the application range of wheat germ meal after extracting wheat germ oil is relatively limited. In addition to being used as cheap feed, it is also used as protein supplement and food ingredient additive. Defatted wheat germ contains up to about 30% protein, and the distribution of amino acids is balanced. The content of eight essential amino acids accounts for about 34% of the total amino acids. It also has great practical significance. For example, using wheat germ to extract bioactive peptides has become one of...

AI Technical Summary

Problems solved by technology

[0004] However, the aforementioned technology is still relatively rudimentary in the research on active peptides from wheat germ, and the active peptides with definite structures and functions cannot be extracted accurately, so they lack practical application value.

Images