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Preparation method of biomolecule microarray based on cellular porous membrane

A biomolecular, honeycomb-like technology, applied in the fields of nanoscience, material chemistry, and biotechnology, can solve problems that do not involve honeycomb-like porous membranes and polydopamine bionic modification, and achieve low cost, controllable scale, and expanded application fields. Effect

Inactive Publication Date: 2014-08-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the above technical documents involve honeycomb porous membrane and polydopamine biomimetic modification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Place a 400-mesh copper mesh with square holes on a glass slide, stabilize a chloroform solution of gold nanoparticles with a particle size of 3 nm with polystyrene with a molecular weight of 250,000 and dodecyl mercaptan (polystyrene concentration is 1.5mg·mL -1 , the concentration of gold nanoparticles was 0.5 mg·mL -1 ) is the film-forming liquid, and the honeycomb porous film is prepared on the copper grid by the water drop template method under the relative humidity condition of 65%;

[0038] (2) Immerse the glass slide with the honeycomb thin film in an aqueous solution of dopamine hydrochloride (1.0 mg·mL -1 , pH 8.5), shake the reaction for 12 hours, then rinse with water for 3 times, and blow dry with nitrogen;

[0039] (3) The copper mesh covered with the honeycomb porous membrane is peeled off from the glass sheet, and the honeycomb porous membrane in the middle of the mesh of the copper mesh is left on the substrate to form a patterned honeycomb porous m...

Embodiment 2

[0042] (1) Place a 400-mesh circular copper mesh on a glass slide, stabilize a chloroform solution of gold nanoparticles with a particle size of 5 nm with polystyrene with a molecular weight of 300,000 and dodecylmercaptan (polystyrene concentration 2.0mg·mL -1 , the concentration of gold nanoparticles was 0.65 mg·mL -1 ) is a film-forming liquid, and a honeycomb-shaped porous film is prepared on a copper grid by using the water drop template method under a relative humidity of 68%;

[0043] (2) Immerse the glass slide with the honeycomb thin film in an aqueous solution of dopamine hydrochloride (1.2 mg·mL -1 , pH 8.5), shake the reaction for 13 hours, then rinse with water for 3 times, and blow dry with nitrogen;

[0044] (3) The copper mesh covered with the honeycomb porous membrane is peeled off from the glass sheet, and the honeycomb porous membrane in the middle of the mesh of the copper mesh is left on the substrate to form a patterned honeycomb porous membrane microar...

Embodiment 3

[0047] (1) Place a 400-mesh copper mesh with square holes on a glass slide, and stabilize a chloroform solution of gold nanoparticles with a particle size of 8 nm (polystyrene concentration: 2.5mg·mL -1 , the concentration of gold nanoparticles was 0.8 mg·mL -1 ) is a film-forming liquid, and a honeycomb-shaped porous film is prepared on a copper grid by using a water drop template method at a relative humidity of 71%;

[0048] (2) Immerse the glass slide with the honeycomb thin film in an aqueous solution of dopamine hydrochloride (1.4 mg·mL -1 , pH 8.5), shake the reaction for 14 hours, then rinse with water for 3 times, and blow dry with nitrogen;

[0049] (3) The copper mesh covered with the honeycomb porous membrane is peeled off from the glass sheet, and the honeycomb porous membrane in the middle of the mesh of the copper mesh is left on the substrate to form a patterned honeycomb porous membrane microarray;

[0050] (4) Immerse the patterned glass slide in an aqueou...

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Abstract

The invention discloses a method for preparing a biomolecular microarray based on a honeycomb porous membrane, and relates to multiple fields such as biotechnology, material chemistry, and nanoscience. The present invention is based on the honeycomb porous film prepared by patterned template, combining the patterning of the honeycomb porous film with the surface functionalization of the film, through the combination of the amino group or sulfhydryl group contained in the biomolecules and the polydopamine modification layer on the surface of the honeycomb porous film Covalent linkage enables patterned microarrays of biomolecules. This method is not only simple to operate, low in cost, and controllable in size, it solves the problem that conventional methods usually require expensive instruments and equipment or special mask materials, and is applicable to the construction of various biomolecular microarrays, and can be used in honeycomb The three-dimensional directional arrangement of biomolecules is realized in the holes, and it is expected to be widely used in the fields of biochips, tissue engineering materials, and biosensors.

Description

technical field [0001] The invention relates to multiple fields such as biotechnology, material chemistry, nanoscience and the like, in particular to a method for preparing a biomolecular microarray based on a honeycomb porous membrane. Background technique [0002] The patterned microarray of biomolecules is a new research direction formed by the interdisciplinary development of many fields such as life science, material science, chemistry and nanoscience. The construction of patterned biomolecular microarrays on the micro-nano scale is of great significance to the development of bioscience technologies such as biochips, microreactors, chemical and biosensors. [0003] The construction of biomolecular microarrays depends on the preparation of patterned substrates and the surface functionalization of microdomains. The current preparation methods for biomolecular microarrays mainly include photolithography, microcontact printing and dip-pen nanolithography. [0004] Photoli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/573G01N33/543G01N33/574
Inventor 马洪敏魏琴朱宝存高丕成范大伟吴丹张勇
Owner UNIV OF JINAN
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