Method for detecting pathogenicity of pathogenic bacteria of banana vascular wilt

Abstract

The invention discloses a method for detecting the pathogenicity of pathogenic bacteria of a banana vascular wilt. The method comprises the following steps: (1) culturing a tissue cultured seedling to be detected in 45ml of water-culturing nutrient solution until the root is 10cm in length, and replacing the water-culturing nutrient solution every 1-4 days; inoculating 5ml of bacterial suspension of to-be-detected pathogenic bacteria of the banana vascular wilt, wherein the conidium concentration of the bacterial suspension is 1*10<5>-1*107>cfu / mL, and culturing for 3 hours at 28 DEG C under a natural lighting condition; (3) using 50ml of fresh water-culturing nutrient solution to replace the original liquid, culturing for 20-25 days at 28 DEG C under a natural lighting condition, and replacing the water-culturing nutrient solution every 1-4 days during the period; (4) cutting up a corm to observe a browning condition, performing statistics to disease indexes and judging and detecting the pathogenicity of pathogenic bacteria of the banana vascular wilt to a to-be-detected banana variety according to the disease indexes. The method can be widely applied to scientific research and production.

Description

technical field [0001] The invention relates to a method for detecting the pathogenicity of the banana fusarium wilt pathogen. Background technique [0002] Banana is the fourth largest food crop in the world after rice, wheat, and corn, and it is also the most traded fresh fruit in the world. my country is one of the main producing areas of bananas. In 2009, the total output of bananas ranked second in the world. The development of the banana industry has made important contributions to the growth of my country's national economy. [0003] Banana Fusarium wilt is a fungal soil-borne vascular disease caused by Fusarium oxysporum Cuban specification. Banana wilt has spread rapidly since it was first reported in Australia in 1874 and has been found in many other banana producing areas. After the disease was discovered for the first time in Taiwan Province of my country in 1967, it became popular in a large area immediately, and it has been found in Hainan, Yunnan, Guangdong, ...

AI Technical Summary

Problems solved by technology

Both of the above two methods have great defects. The banana seedlings in the sand bed may be polluted by the sand, which contains bacteria or root-knot nematodes, etc., resulting in inaccurate experimental results.

The cost of potted banana seedlings is high, which is not conducive to large-scale experiments, and the roots grow in the soil, which is not easy to observe, and the disease is highly dependent on environmental factors

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