Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Identification primers for penis cervi of New Zealand wapiti and penis cervi of sika deer as well as PCR (Polymerase Chain Reaction) identification method

A technology for sika deer and deer penis, which is applied in the fields of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Economic loss and adverse health consequences, simple operation steps, and effect of ensuring drug safety

Inactive Publication Date: 2014-01-22
张敏
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medicinal value of sika deer penis is high but the market price is expensive. New Zealand red deer penis is similar in appearance and shape to sika deer penis and the price is low, but the medicinal value of New Zealand red deer penis is far less than that of sika deer penis. Red deer penis is difficult to distinguish with the naked eye. In order to seek higher economic benefits, some unscrupulous merchants in the market sell New Zealand red deer penis as sika deer penis, which seriously affects the efficacy of deer penis, and causes damage to the health of patients and Economic losses
Due to the complex composition and ingredients of Chinese patent medicines and many interference factors, it is difficult to identify their authenticity and purity with traditional pharmacognosy methods. How to identify the penis of New Zealand red deer and sika deer with good sex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Identification primers for penis cervi of New Zealand wapiti and penis cervi of sika deer as well as PCR (Polymerase Chain Reaction) identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The identification primers for New Zealand red deer whip and sika deer whip are designed according to the 18SrRNA gene sequence of sika deer (GenBank accession number is AY225108). The primer sequence is:

[0028] Upstream primer: 5'-TCTTTGCCGATGGTATAGGT-3';

[0029] Downstream primer: 5'-CGGACGAAACTTTGTGAGATTAA-3'.

[0030] The PCR identification method of New Zealand red deer penis and sika deer penis, its specific steps are as follows:

[0031] 1.1. Genomic DNA extraction method of New Zealand red deer penis and sika deer penis

[0032] New Zealand red deer penis and sika deer penis were treated with alcohol respectively, and 2 g of sika deer penis tissue and 2 g of New Zealand red deer penis tissue were extracted, and New Zealand red deer penile genome DNA and sika deer penis were extracted by phenol-chloroform-isoamyl alcohol extraction method. whip genomic DNA;

[0033] 1.2. PCR primers for amplified samples

[0034] Upstream primer: 5'-TCTTTGCCGATGGTATAGGT-3'...

Embodiment 2

[0046] The identification primers of New Zealand red deer and sika deer penis, the primer sequence is:

[0047] Upstream primer: 5'-TCTTTGCCGATGGTATAGGT-3';

[0048] Downstream primer: 5'-CGGACGAAACTTTGTGAGATTAA-3'.

[0049] The PCR identification method of New Zealand red deer penis and sika deer penis, its specific steps are as follows:

[0050] 1.1. Genomic DNA extraction method of New Zealand red deer penis and sika deer penis

[0051] New Zealand red deer penis and sika deer penis were treated with alcohol respectively, and 2 g of sika deer penis tissue and 2 g of New Zealand red deer penis tissue were extracted, and New Zealand red deer penile genome DNA and sika deer penis were extracted by phenol-chloroform-isoamyl alcohol extraction method. whip genomic DNA;

[0052] 1.2. PCR primers for amplified samples

[0053] Upstream primer: 5'-TCTTTGCCGATGGTATAGGT-3';

[0054] Downstream primer: 5'-CGGACGAAACTTTGTGAGATTAA-3';

[0055] 1.3. PCR reaction system and amplificat...

Embodiment 3

[0065] The identification primers of New Zealand red deer and sika deer penis, the primer sequence is:

[0066] Upstream primer: 5'-TCTTTGCCGATGGTATAGGT-3';

[0067] Downstream primer: 5'-CGGACGAAACTTTGTGAGATTAA-3'.

[0068] The PCR identification method of New Zealand red deer penis and sika deer penis, its specific steps are as follows:

[0069] 1.1. Genomic DNA extraction method of New Zealand red deer penis and sika deer penis

[0070] New Zealand red deer penis and sika deer penis were treated with alcohol respectively, and 2 g of sika deer penis tissue and 2 g of New Zealand red deer penis tissue were extracted, and New Zealand red deer penile genome DNA and sika deer penis were extracted by phenol-chloroform-isoamyl alcohol extraction method. whip genomic DNA;

[0071] 1.2. PCR primers for amplified samples

[0072] Upstream primer: 5'-TCTTTGCCGATGGTATAGGT-3';

[0073] Downstream primer: 5'-CGGACGAAACTTTGTGAGATTAA-3';

[0074] 1.3. PCR reaction system and amplific...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to identification primers for penis cervi of New Zealand wapiti and penis cervi of sika deer as well as a PCR (Polymerase Chain Reaction) identification method, wherein an upstream primer is 5'-TCTTTGCCGATGGTATAGGT-3', and a downstream primer is 5'-CGGACGAAACTTTGTGAGATTAA-3'. The identification method comprises the following steps: extracting genomic DNAs (Deoxyribonucleic Acid) of penis cervi of New Zealand wapiti and penis cervi of sika deer by using a phenol-chloroform-isoamylol extraction method, and the reaction system is that the PCR reaction system is 25 microlitres with components being the upstream and downstream primers, 10*Buffer, dNTP and rTaqDNA polymerase for PCR amplification; carrying out electrophoresis on a PCR amplification product on sepharose gel; determining the authenticity of the penis cervi of New Zealand wapiti and penis cervi of sika deer through electrophoretogram. The identification method is strong in resolving ability and good in repeatability, ensures the medication safety of people, remedies the weakness of animalian traditional Chinese medicine identification in conventional traditional Chinese medicine identification, and provides a scientific basis for clinical rational drug use.

Description

technical field [0001] The invention relates to a primer for identifying New Zealand red deer penis and sika deer penis and a PCR identification method thereof. technical background [0002] Deer Whip, also known as Deer Kidney and Lu Chong, mainly treats diseases such as impotence, tinnitus due to kidney deficiency, women with cold uterus, long-term infertility, and chronic testicular inflammation. The medicinal value of sika deer penis is high but the market price is expensive. New Zealand red deer penis is similar in appearance and shape to sika deer penis and the price is low, but the medicinal value of New Zealand red deer penis is far less than that of sika deer penis. Red deer penis is difficult to distinguish with the naked eye. In order to seek higher economic benefits, some unscrupulous merchants in the market sell New Zealand red deer penis as sika deer penis, which seriously affects the efficacy of deer penis, and causes damage to the health of patients and Econ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2531/113
Inventor 张敏苏玉虹田玉民
Owner 张敏
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com