Self-assembled nano-gene vector compound based on PAMAM (Poly Amido-Amine) and preparation method thereof

A gene carrier and compound technology, applied in the field of pharmaceutical preparations, can solve the problems of low toxicity and side effects, achieve the effects of low toxicity and side effects, simple preparation method, and increase absorption and residence time

Inactive Publication Date: 2014-01-29
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a PAMAM-based self-assembled nano-gene carrier complex for the shortcomings of the existing single PAMAM gene carrier. The gene carrier compound is simple to prepare and can more effectively deliver therapeutic gene drugs to Into the tumor tissue, and improve the absorption and residence time of the drug in the tumor and lymph nodes, thereby improving the efficacy of the drug, while having lower side effects

Method used

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  • Self-assembled nano-gene vector compound based on PAMAM (Poly Amido-Amine) and preparation method thereof
  • Self-assembled nano-gene vector compound based on PAMAM (Poly Amido-Amine) and preparation method thereof
  • Self-assembled nano-gene vector compound based on PAMAM (Poly Amido-Amine) and preparation method thereof

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Embodiment

[0038] Example: The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0039] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0040] Polyamidoamine (PAMAM) dendrimers: with amino groups at the end, ethylenediamine as the core, 4.0 generation, (purchased from Sigma-Aldrich, catalog number: 41244-9, specification 1g, number average molecular weight is 14215 ); adopt 60 ℃ vacuum drying, remove methanol solvent, obtain G4.0PAMAM, then use PBS buffer solution (pH7.4, NaCl8.0g, KCl0.2g, NaCl 2 HPO 4 12H 2 O2.9g, KH 2 PO 4 0.2g, distilled water to 1000mL) dissolved, prepared as a 10mg / mL stock solution and stored at 4°C for later use.

[0041]Hyaluronic acid (HA) was purchased from Shandong Freda Biochemical Co., Ltd., with a specification of 50 g and an average molecular weight of 11000 Da.

[0042] 1. Preparation of HA / PAMAM / siRNA complex

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Abstract

The invention discloses a self-assembled nano-gene vector compound based on PAMAM (Poly Amido-Amine). The self-assembled nano-gene vector compound is a compound system self-assembled from HA (Hyaluronic Acid), PAMAM and a gene under mutual electrostatic interaction, and expressed as HA / PAMAM / gene, wherein PAMAM is selected from polyamidoamine dendrimers of different generations, HA is selected from hyaluronic acids of different molecular weights and sodium salts thereof and the gene is selected from theraputic gene drugs. The compound is capable of effectively prolonging blood circulation time and more effectively transmitting gene drugs into tumor tissues, and simultaneously also capable of protecting the gene drugs from being degraded, and reducing the toxic and side effects of PAMAM; as a result, the curative effect of gene therapy is improved and the shortages of a single PAMAM vector are covered. The invention also provides the preparation method of the compound. The preparation method comprises the steps of: firstly, mixing PAMAM with the aqueous solution of the gene for incubation to form a PAMAM / gene compound under electrostatic adsorption action; next, adding an HA solution, carrying out room-temperature incubation and carrying out self assembling under the electrostatic adsorption action to form the ternary compound HA / PAMAM / gene.

Description

technical field [0001] The invention relates to a gene therapy composition of a non-viral cationic carrier, more specifically, to a PAMAM-based self-assembled nano-gene carrier complex and a preparation method thereof, belonging to the technical field of pharmaceutical preparations. Background technique [0002] In recent years, tumor has become one of the most serious diseases threatening human health after cardiovascular disease. At present, the common treatment methods for tumors are mainly surgery, radiation therapy and chemotherapy. Although these methods can enable patients to obtain a higher survival rate, the problems of postoperative recurrence and distant metastasis are still a major problem that plagues scholars. With the rapid development of molecular biology technology and immunology technology and the deepening of human understanding of tumor pathogenesis, gene therapy has gradually become an important part of tumor biological treatment. [0003] For successf...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/48A61P35/00
Inventor 程丽芳程亮陈大为张璐
Owner SUZHOU UNIV
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