Production method of transmissible gastroenteritis virus vaccine

Abstract

The invention discloses a production method of a transmissible gastroenteritis virus vaccine. The production method comprises the steps of carrying out subculture on cells for production so as to form monolayer cells; multiplying virus seeds for production, namely inoculating the basic virus seeds to the monolayer cells for culturing; dissociating the monolayer cells into a monolayer cell suspension liquid, and inoculating the cell suspension liquid into a bioreactor for culturing; multiplying vaccine culturing virus liquid, namely inoculating the virus seeds for production to the cells to be cultured after the quantity of the cells reaches 5*106-5*107 unit/ml; and harvesting the virus liquid. The production method has the advantages that the production cost can be greatly lowered, the production cycles are short, each production cycle only lasts for 5-7 days, and compared with that of transmissible gastroenteritis virus generated by an existing spinner bottle culture method, the titer of the transmissible gastroenteritis virus produced by the method is higher; the automation degree is high, few workers are required, the production process is simple and stable, the operation is easy, the yield is high, the occupied area is small, the production scale can be easily and rapidly expanded, and the quality is balanced and stable basically; the environmental pollution is slight and is easy to avoid.

Description

technical field [0001] The invention relates to a method for producing porcine transmissible gastroenteritis virus vaccine by using bioreactor microcarrier cell culture, which can be used for industrial production of porcine transmissible gastroenteritis virus vaccine and replaces the traditional rotary bottle culture method. Background technique [0002] At present, the production of porcine transmissible gastroenteritis virus vaccine in China relies on the spinner bottle culture method. The traditional process is labor-intensive, time-consuming, low-efficiency, and high production cost; the cell environment is not uniform, the environmental conditions are difficult to measure and monitor, and it is easy to be polluted by the environment; the difference between different batches of cells is large; it is difficult to expand production; Vaccines produced due to contamination by bacteria or other viruses may have quality problems. In addition, there have been reports on the u...

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Problems solved by technology

The traditional process is labor-intensive, time-consuming, low-efficiency, and high production cost; the cell environment is not uniform, the environmental conditions are difficult to measure and monitor, and it is easy to

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