Application of Sophorolipid in Biological Corrosion Inhibitor for Oilfield Produced Water
A technology of microbial fermentation biosurfactant and produced water, which is applied in the application field of biosurfactants in corrosion inhibitors, and achieves the effects of high application value, low toxicity and simple preparation.
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Embodiment 1
[0022] Embodiment 1: the preparation of sophorolipid
[0023] (1) Strain selection: Candida ( Candida bombicola )ATCC22214;
[0024] (2) Slant culture: inoculate the strains in GYU slant medium supplemented with 20% agar, and culture at 30°C for 48 hours;
[0025] (3) Seed culture: Pick a loop of the strain cultured in step (2) under aseptic conditions with an inoculation loop and place it in 50 mL of GYU liquid medium, and culture it at a constant temperature for 24 hours at 30°C with shaking to obtain a seed culture solution;
[0026] (4) Fermentation culture: Inoculate the above seed culture liquid into 100mL GYU medium with an inoculum volume of 5% by volume, and then add rapeseed oil to the above GYU medium at 0h and 48h of fermentation culture respectively, rapeseed The total volume of oil added is 6% of the volume of the GYU fermentation medium, the fermentation temperature is 30°C, and the total time of constant temperature shaking culture is 120h;
[0027] (5) Coll...
Embodiment 2
[0033] Embodiment 2: Application of sophorolipids of the present invention in biological corrosion inhibitors for oil production water
[0034] (1) Preparation of biological corrosion inhibitor A: Prepare the sophorolipid extract obtained in Example 1 to prepare an aqueous solution with a sophorolipid concentration of 10-50 mg / L;
[0035] (2) Corrosion test: According to the Petroleum and Natural Gas Industry Standard SY / T5273-2000 of the People's Republic of China, the carbon steel test piece and the biological corrosion inhibitor were pretreated, and then the static coupon corrosion test was carried out, and the corrosion rate was finally calculated. The results are shown in the table 2.
[0036] Table 225 ℃, the laboratory corrosion inhibition effect evaluation data of different concentrations of corrosion inhibitor A on carbon steel
[0037] Corrosion inhibitor A concentration (mg / L) Uniform corrosion rate (mm / a) Corrosion inhibition rate (%) blank 0.2...
Embodiment 3
[0039] Embodiment 3: Application of sophorolipids of the present invention in biological corrosion inhibitors for oil production water
[0040] (1) Preparation of biological corrosion inhibitor B: use the supernatant of the fermented broth of saturated sophorolipids obtained in Example 1, and dilute appropriately.
[0041] (2) Corrosion test: According to the Petroleum and Natural Gas Industry Standard SY / T5273-2000 of the People's Republic of China, the carbon steel test piece and the biological corrosion inhibitor were pretreated, and then the static coupon corrosion test was carried out, and the corrosion rate was finally calculated. The results are shown in the table 3.
[0042]Table 325 ° C, different concentrations of corrosion inhibitor B on carbon steel laboratory corrosion inhibition evaluation data
[0043] Corrosion inhibitor B Uniform corrosion rate (mm / a) Corrosion inhibition rate (%) blank 0.2633
[0044] It can be seen from Table 3 that th...
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