Use of SNP site of FoxH1 gene

A technology of foxh1 and 1.foxh1, which is applied to the use of FoxH1 gene SNP sites and related kits, can solve the problems of unconfirmed, undetermined, decreased enzyme activity, etc., and achieve the effect of good sensitivity

Inactive Publication Date: 2014-02-05
谢小冬
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Problems solved by technology

At present, studies on the association between single nucleotide polymorphisms and congenital heart disease are focused on the detection of candidate gene polymorphisms, such as the study of folic acid metabolism pathway genes and susceptibility to congenital heart disease. A SNP (C677T) in the important methylenetetrahydrofolate reductase gene results in reduced enzyme activity, but this conclusion was not confirmed in a meta-analysis
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  • Use of SNP site of FoxH1 gene
  • Use of SNP site of FoxH1 gene
  • Use of SNP site of FoxH1 gene

Examples

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[0057] Example 1

[0058] 1.1 Extract DNA from phenol and chloroform;

[0059] 1. Before extracting DNA, autoclave the items and reagents needed for DNA extraction at 121°C for 15-20 minutes. For example: EP tube, pipette tip, white blood cell lysate, small beaker, red blood cell lysate, sodium acetate solution, TE buffer, oven dried at 65℃.

[0060] 2. Half an hour before DNA extraction, transfer the blood sample (EDTA anticoagulation treatment) from the refrigerator at -20°C to room temperature (equivalent to an air bath) and let it melt slowly.

[0061] 3. Mix whole blood, take 500ml blood sample in 2ml FP tube, add 2-3 times volume of red blood cell lysate, turn upside down, shake for 5min, put in refrigerator for about 2min (equivalent to ice bath), centrifuge after 2min ( 12000rpm10min) to remove the supernatant.

[0062] 4. Add the same amount of erythrocyte lysate in the previous step to resuspend the pellet and centrifuge again (12000rpm for 10min) to discard the supernatant....

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Abstract

The invention discloses a use of an SNP site of an FoxH1 gene. The use means an application of the SNP site rs750472 of the FoxH1 gene in the preparation of a congenital heart disease susceptibility diagnosis kit. The detection kit has a good sensitivity, stability and specificity. The kit has effective risk early-warning and early-stage diagnosis effects when the kit is used for the congenital heart disease susceptibility screening of fetuses in abdominal cavities of pregnant women.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the use of FoxH1 gene SNP sites and related kits. Background technique [0002] Congenital heart disease (abbreviated as congenital heart defects, CHDs) refers to the deformity caused by the developmental defects or partial developmental arrest of the heart and great vessels in the mother during the fetal development period (within the first 2-3 months of pregnancy), Or a birth defect disease in which the channel that should be automatically closed after birth fails to close (it is normal in the fetus). Congenital heart disease (hereinafter referred to as CHDs) is one of the most common diseases among all human birth defects. According to foreign data, the incidence rate of CHDs is 1‰, and the domestic statistics show that the incidence rate accounts for 6.87‰~14.39‰ of newborn babies. [0003] There are many types of congenital heart disease, including simple an...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 谢小冬苏刚李培强吴骅李炯陈晶晶
Owner 谢小冬
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