Use of SNP site of FoxH1 gene
A technology of foxh1 and 1.foxh1, which is applied to the use of FoxH1 gene SNP sites and related kits, can solve the problems of unconfirmed, undetermined, decreased enzyme activity, etc., and achieve the effect of good sensitivity
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[0057] Example 1
[0058] 1.1 Extract DNA from phenol and chloroform;
[0059] 1. Before extracting DNA, autoclave the items and reagents needed for DNA extraction at 121°C for 15-20 minutes. For example: EP tube, pipette tip, white blood cell lysate, small beaker, red blood cell lysate, sodium acetate solution, TE buffer, oven dried at 65℃.
[0060] 2. Half an hour before DNA extraction, transfer the blood sample (EDTA anticoagulation treatment) from the refrigerator at -20°C to room temperature (equivalent to an air bath) and let it melt slowly.
[0061] 3. Mix whole blood, take 500ml blood sample in 2ml FP tube, add 2-3 times volume of red blood cell lysate, turn upside down, shake for 5min, put in refrigerator for about 2min (equivalent to ice bath), centrifuge after 2min ( 12000rpm10min) to remove the supernatant.
[0062] 4. Add the same amount of erythrocyte lysate in the previous step to resuspend the pellet and centrifuge again (12000rpm for 10min) to discard the supernatant....
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