Fluorescence rapid detection method of pfos in environmental water samples

A detection method and water sample technology, applied in the field of environmental analysis, can solve the problems of expensive instruments, long time-consuming, unfavorable popularization, etc., and achieve the effect of low detection cost, fast method and simple operation

Inactive Publication Date: 2016-03-23
SOUTHWEST UNIV
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Problems solved by technology

These methods are not conducive to popularization due to the expensive instruments and the need for professionals to operate them, and take a long time

Method used

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  • Fluorescence rapid detection method of pfos in environmental water samples

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Embodiment Construction

[0018] 1. Qualitative testing

[0019] Detection method: fluorescence analysis.

[0020] Instrument: F-2700 fluorescence spectrophotometer (Hitachi, Japan), used to record and measure fluorescence spectrum and intensity.

[0021] Reagents: PFOS standard substance (purity greater than 98.0%, TCI (Shanghai) Chemical Industry Development Co., Ltd.); prepared mptpy solid, mptpy can be prepared according to existing literature, 4.84g (40mmol) of 2-acetylpyridine ( 2-acetylpyridine) was added to 2.40 g (20 mmol) of 4-methylbenzaldehyde (4-methylbenzaldehyde) in 100 mL of ethanol solution. Then 3.08 g (85%, 40 mmol) of KOH and 60 mL (29.3%) of ammonia solution were added to the solution. After the above solution was stirred and reacted at 34°C for 24 hours, it was cooled to 20°C, filtered to obtain a white colloid, washed with ice-cold ethanol, recrystallized to obtain a white crystal, and vacuum-dried to obtain the product.

[0022] Working solution: directly prepare 5.0×10 with ...

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Abstract

The invention relates to a fluorescent rapid detection method for PFOS (Perfluorooctane Sulfonates) in an environmental water sample and relates to the field of environment analysis. The method comprises the following steps: sequentially adding 50 microliters of a Tris-HCl buffering solution with the pH (Potential of Hydrogen) of 7.70, 35 microliters of an mptpy solution with the concentration of 3.0*10<-4>mol / L and 35 microliters of a ZnSO4 solution with the concentration of 3.0*10<-4>mol / L into a 1.5mL LEP (Lowest Effective Power) colorimetric tube; swirling uniformly and adding a pre-treated water sample to be detected; after swirling uniformly, enabling the volume to be 0.5mL by using 18.2M of ultrapure water; after swirling uniformly, immediately scanning on a fluorospectro photometer, wherein a stimulation slit and an transmission slit are 5nm and the voltage is 400V; recording a fluorescence spectrum with a range of 300nm-550nm; and carrying out primary qualitative analysis and quantitative analysis according to a fluorescence signal at 420nm to obtain whether the environmental water sample contains the PFOS or not. If the water sample contains the PFOS, the fluorescence intensity at the 420nm is weakened; if not, a signal at the 420nm is not changed. The method is used for detecting the PFOS in the environmental water sample, has the characteristics of simplicity and rapidness and is low in detection cost.

Description

technical field [0001] The invention relates to the field of environmental analysis, in particular to a method for detecting PFOS in environmental water samples. Background technique [0002] Perfluorinated compounds are a class of anionic surfactants widely used, from surface protection agents, foam fire extinguishing agents, PTFE, lubricants to insecticides. Perfluorooctanesulfonate (PFOS), as one of them, has attracted widespread attention from researchers because it is the final product of the degradation of many perfluorinated compounds and has been detected in wastewater, surface water, sediment and many wild species. arrive. PFOS and its salts were listed in the Stockholm Convention on Persistent Organic Pollutants in May 2009. Although perfluorooctane sulfonic acid has been banned in most countries, PFOS is still manufactured and is still used in some fields, such as semiconductors, metals, electroplating, hydraulic oil, and photography industries. PFOS has excell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 谭克俊郑莉
Owner SOUTHWEST UNIV
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