Saliva/urine antibody colloidal gold detection technology for hepatitis C virus (HCV) and preparation method

A technology for antibody detection and hepatitis C virus, which is applied in the field of medical detection, can solve the problems of low antibody concentration, unobvious antibody sample protection test results, and inability to perform urine testing, etc., to achieve simple operation, avoid missed detection and false negative phenomena, The effect of less antigen dosage

Inactive Publication Date: 2014-02-05
王勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] The purpose of the present invention is to solve the defects of low HCV antibody concentration in saliva, protection and avoidance of degradation of antibody samples, inconspicuous test...

Method used

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  • Saliva/urine antibody colloidal gold detection technology for hepatitis C virus (HCV) and preparation method
  • Saliva/urine antibody colloidal gold detection technology for hepatitis C virus (HCV) and preparation method
  • Saliva/urine antibody colloidal gold detection technology for hepatitis C virus (HCV) and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The present invention detects human hepatitis C virus (HCV) saliva / urine antibody colloidal gold test strip: such as figure 1 As shown, the bottom plate 11 is included, and the NC film 6 is arranged on the bottom plate 11. The NC film 6 is provided with a C line 7 and a T line 8, and the C line 7 and the T line 8 constitute the detection area 2; one side of the bottom plate 11 is provided with a gold The other side of the conjugate pad 9 is provided with a water-absorbing pad 5; the gold conjugate pad 9 is provided with a sample pad 10 to form a sample loading area 12; the sample loading area 12 is provided with an arrow glue 1; the water-absorbing pad 5 is provided with a Adhesive protective film 3; one end of the water absorption pad 5 is the water absorption area 4.

[0079] Wherein, the colloidal gold conjugate pad 9 that has been coated with nitrocellulose membrane overlaps (2~3mm), and the sample pad 10 that has been sealed that overlaps with the colloidal gold co...

Embodiment 2

[0083] The preparation method of paper strip of the present invention:

[0084] The present invention detects human hepatitis C virus (HCV) saliva / urine antibody colloidal gold test strips using a method for marking the colloidal gold particles, and the diameter of the colloidal gold particles is 30-40nm. The adsorption mechanism of colloidal gold is electrostatic adsorption. Colloidal gold particles reduce gold chloride to a negatively charged colloidal state under the action of a reducing agent, and combine with positively charged proteins to form colloidal gold conjugates. The composition of the urine kit of the present invention, the operation method and the result schematic diagram are shown in Figure 6 ; Human hepatitis C virus (HCV) saliva / urine antibody colloidal gold test strip preparation steps are as follows, see Figure 7 :

[0085] (1) Preparation of colloidal gold: Measure 300 ml of ultrapure water (filtered on the basis of purified water, resistivity: 18.2 MΩ...

Embodiment 3

[0099] Sampling and detection instructions for the detection of human hepatitis C virus (HCV) saliva / urine antibody colloidal gold test strips of the present invention. The present invention utilizes the human hepatitis C virus (HCV) saliva antibody colloidal gold test strip detection of embodiment 1 and example 2. The specific steps of saliva sampling are as follows:

[0100] (1) Open the oral swab for one person, and wipe it repeatedly along the upper gum from left to right for 5-6 seconds, turn over the oral swab and wipe it repeatedly from left to right along the lower gum for 5-6 seconds 6 seconds.

[0101] (2) Insert the sampling swab into the sample diluent and stir it up, down, left, and right for 5-10 seconds to fully release the sample in the saliva, and take it out after wringing dry on the edge of the test tube. The test can be carried out after the sample is collected, and the interval between two samples should be more than 30 minutes, and the sample should be ...

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Abstract

The invention relates to a saliva/urine antibody colloidal gold detection technology for hepatitis C virus (HCV) and a preparation method. According to the technical scheme, a colloidal gold platform system (1) for saliva/urine antibody detection, a target antibody protection system (a saliva sample diluent) (2) for saliva antibody detection, a sealing system (3) for the saliva/urine antibody detection, an antigen screening system (4) for the saliva/urine antibody detection and a drying system (5) for the saliva/urine antibody detection are adopted. According to the technology and the method, the detection rate of a target antibody is improved, the specificity of a product is ensured, and the phenomena of detection missing and false negative are effectively avoided. The technology is easy to operate, safe, pollution-free, and suitable for individual detection, household detection and bedside detection. A sample is easily and conveniently taken, an operator is free from secondary infection, and a patient or a high risk group can be self-detected.

Description

technical field [0001] The invention relates to a human hepatitis C virus (HCV) saliva / urine antibody colloidal gold detection technology and a preparation method thereof, belonging to the technical field of medical detection. Background technique [0002] In 1974, Golafield first reported non-A non-B hepatitis after blood transfusion. In 1989, Choc et al obtained the gene clone of the virus by using molecular cloning technology, and named the disease and its virus as hepatitis C (Hepatitis C) and hepatitis C virus (HCV). Because the HCV genome is similar to human flaviviruses and pestiviruses in structure and phenotypic characteristics, it is classified as Flaviviridae HCV. The HCV virion is spherical, with a diameter of less than 80nm (36-40nm in hepatocytes and 36-62nm in blood). There are spikes on the membrane. HCV in vitro culture has not yet found a sensitive and effective cell culture system, but chimpanzees are very sensitive to HCV. [0003] The pathogenesis of...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/531
CPCG01N33/532G01N33/56983G01N33/5767G01N2333/186G01N2469/00G01N2800/7095
Inventor 王勇
Owner 王勇
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