Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Procalcitonin latex enhanced immunoturbidimetry detection kit

A detection kit, latex-enhanced technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of narrow linear range, large subjective error, high price, etc., to shorten the reaction time, increase the sensitivity, and ensure stability. sexual effect

Inactive Publication Date: 2014-02-05
CHONGQING ZHONGYUAN BIOLOGICAL TECH
View PDF8 Cites 37 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For PCT detection, existing detection methods mainly include quantitative methods, semi-quantitative detection methods and qualitative detection methods. Quantitative detection methods include double antibody sandwich method (ELISA), immunoluminescence method, high performance liquid chromatography analysis, radioimmunoassay, etc. Among them, radioimmunoassay and high performance liquid chromatography molecular method have certain limitations in clinical practice, while ELISA (double antibody sandwich method) and immunoluminescence method are commonly used in clinical practice. ELSA has high sensitivity, but its operation is complicated and requires more Time, the general results are qualitative or semi-quantitative, the quantitative deviation is large, and the linear range is narrow, it is not easy to control the dilution of the sample; the immunochemiluminescence method has the advantage of high sensitivity, which can meet the clinical needs of PCT determination, but it needs supporting special The high price of the instrument is not conducive to hospital promotion, and the standard curve will drift with time (the luminescence lasts for a long time and can be measured at different times)
Qualitative methods mainly include immunochromatography, which can be used as a qualitative or semi-quantitative detection method, and can quickly give results. No special equipment is required for qualitative methods, but quantitative data cannot be given, and subjective errors are relatively large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Procalcitonin latex enhanced immunoturbidimetry detection kit
  • Procalcitonin latex enhanced immunoturbidimetry detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A procalcitonin latex-enhanced immune turbidimetric detection kit, comprising the following preparation steps:

[0033] (1) Preparation of reagent R1:

[0034]Weigh 23.83g Hepes, 9g NaCl, 15g PEG8000, 0.5g sodium azide, 5g bovine serum albumin and 14.21g EDTA, dissolve them in 0.8L distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.

[0035] (2) Preparation of reagent R2:

[0036] Step 1: Add PBS buffer solution with a pH of 7.4 and a concentration of 100mmol / L to the mouse anti-human PCT antibody for dialysis at 4°C, and complete the dialysis after changing the water 3 times in the middle. Dilute the human PCT antibody to a mouse anti-human PCT antibody diluent with a concentration of 2 mg / ml; wash the polystyrene latex microspheres whose surface functional groups are carboxyl groups with distilled water.

[0037] Step 2: Dilute the washed polystyrene latex microspheres to a mass concentration of 1% with PBS buffer solution with a pH of 7.4 a...

Embodiment 2

[0039] A procalcitonin latex-enhanced immune turbidimetric detection kit, comprising the following preparation steps:

[0040] (1) Preparation of reagent R1:

[0041] Weigh 23.83g Hepes, 30g NaCl, 100g PEG8000, 0.5g sodium azide, 50g bovine serum albumin and 14.21g EDTA, dissolve them in 0.8L distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.

[0042] (2) Preparation of reagent R2:

[0043] Step 1: Add PBS buffer solution with a pH of 7.4 and a concentration of 500mmol / L to the mouse anti-human PCT antibody for dialysis at 4°C, and complete the dialysis after changing the water 3 times in the middle. Dilute the human PCT antibody to a mouse anti-human PCT antibody diluent with a concentration of 2 mg / ml; wash the polystyrene latex microspheres whose surface functional groups are carboxyl groups with distilled water.

[0044] Step 2: Dilute the washed polystyrene latex microspheres to a mass concentration of 1% with PBS buffer solution with a pH of 7...

Embodiment 3

[0046] A procalcitonin latex-enhanced immune turbidimetric detection kit, comprising the following preparation steps:

[0047] (1) Preparation of reagent R1:

[0048] Weigh 23.83g Hepes, 20g NaCl, 80g PEG8000, 0.5g sodium azide, 50g bovine serum albumin and 14.21g EDTA, dissolve them in 0.8L distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.

[0049] (2) Preparation of reagent R2:

[0050] Step 1: Add Hepes buffer solution with a pH of 7.4 and a concentration of 500mmol / L to the mouse anti-human PCT antibody for dialysis at 4°C, and complete the dialysis after changing the water 3 times in the middle, and use 500mmol / L Hepes buffer solution to dissolve the mouse antibody Dilute the human PCT antibody to a mouse anti-human PCT antibody diluent with a concentration of 2 mg / ml; wash the polystyrene latex microspheres whose surface functional groups are carboxyl groups with distilled water.

[0051] The second step: the above-mentioned washed polystyren...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a procalcitonin (PCT) latex enhanced immunoturbidimetry detection kit and a preparation method thereof. The procalcitonin latex enhanced immunoturbidimetry detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 mainly comprises a buffer solution 1, a stabilizing agent 1, a preservative 1, a coagulation increasing agent, a protective agent 1 and EDTA (Ethylene Diamine Tetraacetic Acid); the reagent R2 mainly comprises a buffer solution 2, a stabilizing agent 2, a preservative 2, a polystyrene latex microballon sphere, a PCT antibody and a protective agent 2; the particle size of the polystyrene latex microballon sphere in the reagent R2 is 100-600nm, the PCT antibody is one or more of a mouse anti-human PCT antibody, a goat anti-human procalcitonin antibody or a rabbit anti-human procalcitonin antibody, and the PCT antibody is connected with the polystyrene latex microballon sphere in a covalent coupling or physical absorption manner. Compared with the prior art, the PCT detection kit provided by the invention has the advantages of low preparation cost, good stability, high detection sensitivity and strong specificity, is easy to store, and is easily popularized in clinical.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a procalcitonin latex enhanced immune turbidimetric detection kit. Background technique [0002] Procalcitonin (PCT) is a glycoprotein composed of 116 amino acids, which is the precursor peptide of calcitonin (CT). Procalcitonin can be enzymatically cleaved into many small fragments, eventually forming aminoprocalcitonin, mature calcitonin and calcitin. Procalcitonin can exist in normal human serum in free form. Under normal circumstances, the serum PCT level in the human body is very low, most of which are lower than 0.1ng / ml, and 0.5ng / ml is the cut-off value for the diagnosis of systemic infection (sepsis); PCT is physiologically increased within 2 days of birth, and the highest It can reach 21ng / ml; the plasma PCT value of long-term hemodialysis patients can reach 1.5ng / ml. Procalcitonin can increase 2-3 hours after infection, reach the peak after 6-12 hours, ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/54393G01N33/54313
Inventor 李民友潘能科高昂
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products