Procalcitonin latex enhanced immunoturbidimetry detection kit
A detection kit, latex-enhanced technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of narrow linear range, large subjective error, high price, etc., to shorten the reaction time, increase the sensitivity, and ensure stability. sexual effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] A procalcitonin latex-enhanced immune turbidimetric detection kit, comprising the following preparation steps:
[0033] (1) Preparation of reagent R1:
[0034]Weigh 23.83g Hepes, 9g NaCl, 15g PEG8000, 0.5g sodium azide, 5g bovine serum albumin and 14.21g EDTA, dissolve them in 0.8L distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.
[0035] (2) Preparation of reagent R2:
[0036] Step 1: Add PBS buffer solution with a pH of 7.4 and a concentration of 100mmol / L to the mouse anti-human PCT antibody for dialysis at 4°C, and complete the dialysis after changing the water 3 times in the middle. Dilute the human PCT antibody to a mouse anti-human PCT antibody diluent with a concentration of 2 mg / ml; wash the polystyrene latex microspheres whose surface functional groups are carboxyl groups with distilled water.
[0037] Step 2: Dilute the washed polystyrene latex microspheres to a mass concentration of 1% with PBS buffer solution with a pH of 7.4 a...
Embodiment 2
[0039] A procalcitonin latex-enhanced immune turbidimetric detection kit, comprising the following preparation steps:
[0040] (1) Preparation of reagent R1:
[0041] Weigh 23.83g Hepes, 30g NaCl, 100g PEG8000, 0.5g sodium azide, 50g bovine serum albumin and 14.21g EDTA, dissolve them in 0.8L distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.
[0042] (2) Preparation of reagent R2:
[0043] Step 1: Add PBS buffer solution with a pH of 7.4 and a concentration of 500mmol / L to the mouse anti-human PCT antibody for dialysis at 4°C, and complete the dialysis after changing the water 3 times in the middle. Dilute the human PCT antibody to a mouse anti-human PCT antibody diluent with a concentration of 2 mg / ml; wash the polystyrene latex microspheres whose surface functional groups are carboxyl groups with distilled water.
[0044] Step 2: Dilute the washed polystyrene latex microspheres to a mass concentration of 1% with PBS buffer solution with a pH of 7...
Embodiment 3
[0046] A procalcitonin latex-enhanced immune turbidimetric detection kit, comprising the following preparation steps:
[0047] (1) Preparation of reagent R1:
[0048] Weigh 23.83g Hepes, 20g NaCl, 80g PEG8000, 0.5g sodium azide, 50g bovine serum albumin and 14.21g EDTA, dissolve them in 0.8L distilled water, adjust the pH to 7.4, and dissolve 1L to obtain reagent R1.
[0049] (2) Preparation of reagent R2:
[0050] Step 1: Add Hepes buffer solution with a pH of 7.4 and a concentration of 500mmol / L to the mouse anti-human PCT antibody for dialysis at 4°C, and complete the dialysis after changing the water 3 times in the middle, and use 500mmol / L Hepes buffer solution to dissolve the mouse antibody Dilute the human PCT antibody to a mouse anti-human PCT antibody diluent with a concentration of 2 mg / ml; wash the polystyrene latex microspheres whose surface functional groups are carboxyl groups with distilled water.
[0051] The second step: the above-mentioned washed polystyren...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com