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Technology for culturing bird-sourced cordyceps

A bird Cordyceps, Cordyceps technology, applied in the bioengineering field of artificially infecting birds' eggs with Cordyceps fungus to cultivate Cordyceps, can solve the problems of high production cost, high technical content, low commodity value, etc., and achieve convenient operation and good taste , the effect of high edible value

Inactive Publication Date: 2015-04-22
YANTAI JINGANG SHENGYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] 1. Due to the poor taste, it can only be used as a raw material for the production and processing of medicines and health care products, and cannot be eaten directly as a dish
[0015] 2. Cordyceps itself sells poorly, and the commodity value is low
[0016] 3. High production cost and high technical content (except for the production of Cordyceps mycelium and fruiting body of Cordyceps sinensis), and the content of active ingredients is lower than that of naturally growing Cordyceps sinensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The technique of cultivating bird Cordyceps comprises the following steps

[0051] 1. Breeding bacteria

[0052] (1) Cultivate the female parent species

[0053] Collect wild Cordyceps strains, use sexual reproduction technology to extract spores to obtain Cordyceps female parent spores, and then use robust cell reproduction technology to rejuvenate Cordyceps female parent spores, and then undergo multiple domestication and breeding experiments, and finally obtain Cordyceps female parent species. Store for later use. The inventor went to Northeast Changbai Mountains in September 2001 to collect wild Cordyceps strains. After inspection, it was determined that the strains were Paecilomyces Cordyceps strains, and the female parent species has been inherited so far.

[0054] (2) Cultivate regenerated mother species

[0055] Agar Medium Recipe:

[0056] Sucrose 30g, agar 15g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.3g, potassium chloride 0.5g, ferric sulfa...

Embodiment 2

[0084] (1) Culture during the infection period

[0085] Dark light conditions, temperature 18°C, humidity 55%, ventilation for 10 minutes in the morning and evening, cultured for 62 days.

[0086] (2) Cultivation during the color change period

[0087] 200 lux light irradiation, temperature 19°C, humidity 70%, ventilation for 25 minutes in the morning and evening, and culture for 15 days.

[0088] (3) Cultivation at the grass emergence stage

[0089] Early training

[0090] Natural light, temperature 19°C, humidity 75%, ventilated for 30 minutes in the morning and evening, cultured for 8 days, and the growth of the subunits reached 2cm, and the pre-cultivation ended.

[0091] Mid-term training

[0092] Natural light, temperature 20°C, humidity 80%, ventilated for 40 minutes in the morning and evening, cultured for 10 days, the growth of sacrum to 4cm, the mid-term culture is over.

[0093] Post-cultivation

[0094] Natural light, temperature 20°C, humidity 85%, ventilate...

Embodiment 3

[0096] (1) Culture during the infection period

[0097] Dark and light conditions, temperature 19°C, humidity 53%, ventilation for 10 minutes in the morning and evening, cultured for 62 days.

[0098] (2) Cultivation during the color change period

[0099] 200 lux light irradiation, temperature 19°C, humidity 67%, ventilation for 20 minutes in the morning and evening, and culture for 13 days.

[0100] (3) Cultivation at the grass emergence stage

[0101] Early training

[0102] Natural light, temperature 18°C, humidity 73%, ventilated for 30 minutes in the morning and evening, cultured for 9 days, the growth of the subunits reached 1.5cm, and the pre-cultivation ended.

[0103] Mid-term training

[0104] Natural light, temperature 18°C, humidity 78%, ventilated for 28 minutes in the morning and evening, cultured for 10 days, when the sacrum grows to 3cm, the mid-term culture is over.

[0105] Post-cultivation

[0106] Natural light, temperature 20°C, humidity 85%, ventil...

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Abstract

The invention provides a technology for culturing bird-sourced cordyceps. The technology for culturing the bird-sourced cordyceps is characterized by comprising the following steps: 1, culturing strains: (1) collecting wild cordyceps strains, performing sexual reproduction, rejuvenation, acclimation and germination to obtain cordyceps female parent, and storing the pure cordyceps strains for later use, (2) culturing regeneration female parents, and (3) culturing liquid strains; 2, selecting bird eggs as hosts, and sterilizing the bird eggs; 3, inoculating; 4, culturing: (1) culturing in contamination period, (2) culturing in veraison period, and (3) culturing in germination period, wherein the process of culturing in germination period is carried out in earlier, middle and later stages; till completion of the culture, the culture time for completing one growth cycle of the bird-sourced cordyceps is about 100 days. The culturing method is simple in operation and is suitable for large-scale production; active components of the cultured cordyceps are not less than those of natural cordyceps; the cultured cordyceps has good taste and can be directly eaten as food.

Description

technical field [0001] The invention relates to a process for cultivating Cordyceps with high-protein poultry eggs with reproductive function and edible standards, and belongs to the technical field of bioengineering for artificially infecting poultry eggs with Cordyceps fungus to cultivate Cordyceps. Background technique [0002] Cordyceps sinensis is a traditional precious nourishing Chinese herbal medicine, which belongs to the phylum Fungi, Ascomycetes, Hypocreales, Ergotaceae, and Cordyceps. According to modern research results of pharmacology, Cordyceps sinensis contains about 7% of cordycepic acid, 28.9% of carbohydrates, about 8.4% of fat, about 25% of protein, and 82.2% of fat is unsaturated fatty acid. In addition, it also contains vitamin B12, ergosterol, Six-carbon sugar alcohols, alkaloids, etc., have various effects such as regulating immune system function, anti-tumor, and anti-fatigue. [0003] Cordyceps sinensis was mostly wild in the past. With the increas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 李金刚张群喜李春生
Owner YANTAI JINGANG SHENGYUAN BIOTECH
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