Method for segmented purification of beta-sitosterol and stigmasterol by using n-amyl alcohol

Abstract

A method for segmented purification of beta-sitosterol and stigmasterol by using n-amyl alcohol comprises the following steps: (1) mixing and dissolving blended phytosterol and n-amyl alcohol at the temperature of 60-70 DEG C, then adding beta-sitosterol to prepare a beta-sitosterol saturated solution at the temperature, thermally filtering, cooling a filter liquor, stirring at a constant temperature, preserving heat and filtering to obtain a crude product of beta-sitosterol; (2) continuing to cooling the filter liquor, stirring at the constant temperature, preserving heat and filtering to obtain a crude product of blended sterol; (3) heating and dissolving the crude product of blended sterol by using the n-amyl alcohol to prepare a stigmasterol saturated solution, cooling, stirring at the constant temperature, preserving heat and filtering to obtain a crude product of stigmasterol; (4) heating and concentrating the filter liquors in the step (2) and the step (3), cooling, stirring at the constant temperature, preserving heat and filtering to obtain the crude product of beta-sitosterol; (5) refining the crude product of stigmasterol by using the n-amyl alcohol; (6) refining the crude product of beta-sitosterol by using the n-amyl alcohol. The method provided by the invention is simple in steps, a single solvent is easy to recover and does not cause pollution, and the product purity and the yield are high.

Description

technical field

[0001] The invention relates to a method for segmentally purifying β-sitosterol and stigmasterol by using n-pentanol. Background technique

[0002] Phytosterol is a natural active substance present in plants, mainly including β-sitosterol, stigmasterol and campesterol. They have the same chiral structure, and the only difference in structure is the side chain. β-sitosterol, also known as sitosterol, has the effects of lowering cholesterol, relieving cough, anticancer, and anti-inflammation; it can be used as a drug for regulating hypercholesterolemia, alleviating atherosclerosis, and preventing and treating prostate diseases. It has obvious curative effect on breast cancer and can also be used as an anti-inflammatory drug and a drug against chronic acetic acid gastric ulcer. Stigmasterol has the function of significantly lowering human serum cholesterol, preventing cerebral hemorrhage and arteriosclerosis. It is used for blood lipid regulation and anti-ather...