Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel hecogenin compound and extract and preparation method and application thereof

A technology of helicoside and helicoside, which is applied in the field of Liliaceae Chlorophytum plant extract and its preparation, and can solve problems such as chemical composition research

Active Publication Date: 2014-02-19
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no one has studied its chemical composition

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel hecogenin compound and extract and preparation method and application thereof
  • Novel hecogenin compound and extract and preparation method and application thereof
  • Novel hecogenin compound and extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of active ingredient monomer neohecoside (extraction and separation process):

[0045] The whole herb of C.tuberosum was provided by Abdulai Jawo Bah, an African student at the International College of Tianjin University of Traditional Chinese Medicine. It was brought back from Africa, about 1.5kg, and extracted 3 times with 8 times the amount of 50% ethanol, 2 hours each time, and combined with 50% ethanol Extraction solution, recover ethanol under reduced pressure until it has no alcohol smell. Add distilled water to dilute to 4L, filter and perform macroporous resin column chromatography, water-ethanol gradient elution (water-95% ethanol, v / v), and recover the solvent by distillation under reduced pressure to obtain the following five components: 30% ethanol Eluate 60g, 50% ethanol eluate 20g, 70% ethanol eluate 2.7g, 95% ethanol eluate 1.4g.

[0046] The 70% ethanol eluate was subjected to ODS column chromatography (water-100% acetonitrile gradient elutio...

Embodiment 2

[0063] Effects of the new jellyfish saponin compound and C.tuberosum extract of the present invention on platelet aggregation

[0064] The in vitro platelet aggregation activity test system was used to test the effects of components and some compounds obtained from C.tuberosum 50% ethanol extract separated by macroporous resin column on platelet aggregation. The results showed that 70% ethanol eluate had the activity of inducing platelet aggregation. The 95% ethanol eluate has inhibitory activity on ADP-induced platelet aggregation.

[0065] 1 Experimental Instruments and Reagents

[0066] laboratory apparatus:

[0067] Flexstation3 microplate reader (Molecular Device); high-speed centrifuge (Thermo Electron Corporation, USA, Thermo-RC6 + ); 96-well ELISA plate, ten-thousandth balance (METTLER TOLEDO Instrument Shanghai Co., Ltd., AL204), platelet mirror; vibrating stirrer (Jintan Medical Instrument Factory, XH-C); pipette gun (1000μl, 200μl , 20μl, 10μl, 2.5μl, Eppendorf, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a novel hecogenin compound with a structural formula (I): (novel hecogenin,3-O-beta-D-glucose-(1-2)-[beta-D-glucose-(1-3)]-beta-D-glucose-(1-4)-beta-D-galactoside); after the reflowing extraction of C.tuberosum harb ethanol, obtained crude extract is implemented with column chromatography by macroporous resin, and is gradually eluted by water and ethanol; the obtained 70% ethanol eluate is implemented with ODS column chromatography, and is eluted by water and 100% acetonitrile; the 70% ethanol eluate has the purpose of inducing platelet aggregation activity aspect.

Description

technical field [0001] The invention relates to a plant extract of the genus Chlorophytum of Liliaceae and its preparation method and application, especially the saponin compound and extract of the genus Chlorophytum and its preparation method and application. Background technique [0002] Platelet aggregation and thrombosis are important causes of ischemic cardio-cerebral injury, and anti-platelet aggregation therapy can help reduce the occurrence of cardiovascular and cerebrovascular adverse events. The anti-platelet aggregation effect of steroidal saponins is one of its mechanisms of action as a drug for treating cardiovascular and cerebrovascular diseases, atherosclerosis, myocardial infarction and other diseases. [0003] Steroidal saponins are an important class of active ingredients in plants. They are widely used clinically as drugs for the treatment of cardiovascular and cerebrovascular diseases. Their indications include coronary heart disease, myocardial ischemia,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07J71/00A61K31/7048A61P7/04
Inventor 张鹏吴红华田晓轩付爱珍吴学芹朱彦高秀梅刘二伟
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com