Narcissus BES-SSR marker primer CATACA5 and method for identifying narcissus variety

Abstract

The invention relates to a narcissus BES-SSR marker primer CATACA5 and a method for identifying a narcissus variety. The method comprises design and synthesis of the narcissus BES-SSR marker primer CATACA5, extraction of narcissus genome total DNA, establishment of a total PCR (polymerase chain reaction) system, execution of a PCR amplification program, preparation of polyacrylamide gel electrophoresis conditions and detection and identification of a PCR amplification product. The primer adopts a narcissus BES-SSR marker developed by a narcissus genome BAC-tail sequence and has the characteristics of high accuracy, easiness in judgment, good repeatability, avoidance from influence of environmental factors, clear spectrum band and the like, is an efficient and reliable molecular marker and used for identifying the narcissus variety and has the advantages of small sampling amount, convenience in operation, reliable result and the like. The narcissus BES-SSR marker primer CATACA5 can be applied to technical fields such as narcissus variety fingerprint drawing, genetic diversity analysis, social genetic structure researching, genetic linkage map analysis and the like.

Description

technical field [0001] The invention relates to a BES-SSR marker primer and a method for identifying a crop variety thereof, in particular to a narcissus BES-SSR marker primer CATACA5 and a method for identifying a narcissus variety, belonging to the field of molecular biology. Background technique [0002] Narcissus is a genus of Amaryllidaceae Narcissus ( Narcissus L .) Perennial flower bulbs, most of which are native to the Mediterranean coast and central Europe. There are about 40 native species and many varieties. According to the statistics of the Royal Horticultural Society, there are now more than 10,000 horticultural varieties. It is very rich, and some daffodils have a pleasant fragrance, and have become famous ornamental and medicinal flowers widely cultivated in countries all over the world. However, narcissus has the characteristic of dormancy in summer and blooms once a year. It takes a long time to identify the flower type and color, which is time-consuming a...

AI Technical Summary

Problems solved by technology

[0006] At present, the developed SSR markers mainly include G-SSR (genome SSR) and EST-SSR (expressed sequence tag SSR). However, there are still few narcissus EST sequences registered in the existing narcissus EST database, and the development of SSR markers is far away. Insufficient, and the polymorphism of SSR markers obtained by using EST sequences is lower than that of genomic SSR markers. Therefore, the information of SSR loci is obtained from the narcissus genome, and the SSR marker primers based on the narcissus genome are developed to identify narcissus germplasm and analyze genetic diversity. The field provides efficient labeling techniques that are imminent

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