Application of anthraquinone or anthraquinone derivatives as matrix in matrix assisted ultraviolet-visible light laser desorption / ionization mass spectrometry

A technology of laser desorption ionization and anthraquinone derivatives, which is applied in the fields of environmental analysis, mass spectrometry detection, and metabolomics, and achieves the effects of good sample compatibility, reduced requirements, and wide practical significance.

Active Publication Date: 2014-02-26
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difference in laser light sources, many solutions suitable for UV-MALDI mass spectrometry cannot solve the problem of Vis-MALDI mass spectrometry very well.

Method used

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  • Application of anthraquinone or anthraquinone derivatives as matrix in matrix assisted ultraviolet-visible light laser desorption / ionization mass spectrometry
  • Application of anthraquinone or anthraquinone derivatives as matrix in matrix assisted ultraviolet-visible light laser desorption / ionization mass spectrometry
  • Application of anthraquinone or anthraquinone derivatives as matrix in matrix assisted ultraviolet-visible light laser desorption / ionization mass spectrometry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the analysis of oligosaccharides

[0037] Take various prepared sample solutions (maltose, maltotetraose and maltopentaose) and matrix solution and mix them uniformly at a volume ratio of 1:1 (wherein the molar ratio of matrix to maltose, maltotetraose and maltopentaose is 1000 :1, 1000:1 and 1000:1), then add 1 μL of the mixed sample on the ITO glass plate, dry it in the air and enter the mass spectrometry analysis. The wavelength of the light source is 532nm, the voltage of the ITO glass plate is 1.0kV, the temperature of the ion transfer tube is 275°C, the voltage of the lens is 40V, and the positive ion mode is used.

[0038] In the AP-Vis-MALDI ionization mode, the matrix can transfer protons to the analyte compounds (maltose, maltotetraose and maltopentaose), making the analyte compounds positively charged and detected by mass spectrometry.

[0039] The mass spectrometry figure that present embodiment obtains is as image 3 It can be seen from the ...

Embodiment 2

[0040] Embodiment 2, the analysis of polypeptide substance

[0041] Take various prepared sample solutions (tetrapeptide GPRP, hexapeptide GRGDTP and decapeptide TLSRRTRFHT) and matrix solution and mix them uniformly at a volume ratio of 1:1 (wherein, the moles of matrix and tetrapeptide GPRP, hexapeptide GRGDTP and decapeptide TLSRRTRFHT The ratios are 1000:1, 1000:1, and 1000:1), and then add 1 μL of the mixed sample to the ITO glass plate, dry it in the air, and enter the mass spectrometry analysis. The wavelength of the mass light source is 532nm, the voltage of the ITO glass plate is 1.0kV, the temperature of the ion transfer tube is 275°C, the voltage of the lens is 40V, and the positive ion mode is used.

[0042] In the AP-Vis-MALDI ionization mode, the matrix can transfer protons to the test compound (tetrapeptide GPRP, hexapeptide GRGDTP, decapeptide TLSRRTRFHT), so that the test compound is positively charged and detected by mass spectrometry.

[0043] The mass spec...

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PUM

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Abstract

The invention discloses an application of anthraquinone or an anthraquinone derivative as a matrix in matrix assisted ultraviolet-visible light laser desorption / ionization mass spectrometry (MALDI). The anthraquinone derivative is any one of the following 1) to 3): 1) a substituted anthraquinone substituted by at least one of the following groups: alkyl, amino, halogen, nitro, carboxyl, aldehyde group, carbonyl, aryl, cyan, and quaternary ammonium group; 2) a salt formed by metal ions and substituted anthraquinone substituted by sulfo group or carboxyl group, wherein the metal ions are sodions, potassium ions, calcium ions, or magnesium ions; 3) a salt formed by inorganic acid or organic acid and substituted anthraquinone substituted by at least one of the following groups: alkyl, amino, halogen, nitro, carboxyl, aldehyde group, carbonyl, aryl, cyan, and quaternary ammonium group. The invention technically overcomes the defect that recently-reported ultraviolet-visible light matrixes are easy to cause severe matrix background interference phenomena in the low molecular weight zone and thus lead to ineffective analysis of micromolecular samples.

Description

technical field [0001] The invention relates to the application of anthraquinone or anthraquinone derivatives as a matrix in matrix-assisted ultraviolet-visible laser desorption ionization mass spectrometry, and belongs to the fields of metabolomics, environmental analysis, mass spectrometry and the like. Background technique [0002] Since Karas et al. and Tanaka et al. reported in 1988 that matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology can effectively analyze biological macromolecules and organic synthetic polymer mass spectrometry, MALDI-TOF MS The technology is favored by researchers from all over the world. The commonly used nitrogen molecular laser (337nm) and frequency tripled Nd:YAG laser (355nm) have better performance in MALDI mass spectrometry compared with other band lasers, so most of the commercial MALDI mass spectrometers currently use ultraviolet lasers. substrates, such as α-cyano-4-hydroxycinnamic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
Inventor 聂宗秀何清陈素明熊彩侨方晓红万立骏
Owner INST OF CHEM CHINESE ACAD OF SCI
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