Ultrasonic-assisted enzymatic hydrolysis method for extracting protein from camellia oleifera seed meal

Abstract

The invention relates to an ultrasonic-assisted enzymatic hydrolysis method for extracting camellia seed meal protein, comprising the following steps: (1) enzymatic hydrolysis: crushing camellia seed meal powder that has passed through a 80-100 mesh sieve with a material-liquid ratio of 1:6-7 and Mix the water evenly, adjust the pH value to 7.25~7.30, add compound plant hydrolytic enzyme with 0.04~0.05% of the mass of camellia seed meal powder, enzymolyze at 65~70℃ for 3~3.5h, inactivate the enzyme after enzymolysis, and then centrifuge to obtain Camellia oleifera seed meal residue; (2) Ultrasonic-assisted alkaline extraction: mix the Camellia oleifera seed meal residue with appropriate amount of water evenly, adjust the pH value to 10.0~10.5, and carry out alkaline extraction at 40~45°C for 80~90min. Auxiliary, the ultrasonic power is 300~1000W, the ultrasonic time is 20min, and the supernatant is obtained by centrifugation after the alkali extraction is completed; (3) Acid precipitation: adjust the pH value of the supernatant obtained in step (2) to 4.6, and store it at room temperature Stand still for 1-1.5h, and then centrifuge to obtain a precipitate, and the precipitate is freeze-dried to obtain a camellia seed meal protein. The extraction process of the invention has mild conditions, and the prepared camellia seed meal has good protein shape and color, and can be widely used as a food additive.

Description

technical field [0001] The invention relates to a protein extraction method, in particular to an ultrasonic-assisted enzymolysis method for extracting camellia oleifera seed meal protein. Background technique [0002] Camellia oleifera (Camellia oleifera) is an evergreen small tree belonging to the family Theaceae and the genus Camellia. It is an excellent woody oil plant in the southern hilly areas of my country. my country is a big tea-producing country, and the planting area of ​​camellia oleifera alone reaches 4 million hm 2 , is the country with the most varieties of Camellia oleifera, the widest distribution and the highest yield of Camellia oleifera seeds in the world, with an annual output of about 549,800 tons of Camellia oleifera seeds, 200,000 tons of camellia oil, and an average annual output of 397,100 tons of Camellia oleifera by-product—Camellia oleifera meal . The protein content in Camellia oleifera seed meal is about 12%-15%, and its amino acid composition...

AI Technical Summary

Problems solved by technology

In the prior art, the camellia oleifera seed meal protein is mostly extracted by alkali-soluble acid precipitation method, but the extraction efficiency of this method is low, and the shape and color of the extracted camellia seed meal protein are poor, which is not conducive to the use of the camellia oleifera seed meal protein as a food additive.