Method for determining solanesol filter interception in mainstream smoke

Abstract

The invention discloses a method for determining the solanesol filter interception in mainstream smoke, which comprises the following steps: 1) preparation of a standard stock liquid: weighing about 50.0mg of solanesol standard reagent, and preparing solanesol series standard solutions in series concentration gradient by use of methanol; 2) sample preparation: sucking the cigarette according to GB / T19609, longitudinally cutting four filters after the suction and putting into a 50mL conical flask, adding 20mL of isopropyl alcohol and extracting on a mechanical oscillator for 1 hour, enabling the extracted solution to pass through a 0.45-micron organic phase filter membrane, sampling and performing high performance liquid chromatography analysis, determining the nature by comparing the retention time of the chromatographic peaks of the standard sample and the filter sample, and determining the quantity by an external standard method. The method disclosed by the invention has the advantages that the sample pretreatment process is simple and quick; the method has high sensitivity, good precision and recovery rate and good stability, can realize determination of the interception of solanesol in the cigarette filters of different filter materials such as cellulose acetate, polypropylene fiber, active carbon, paper filter and the like, and has relatively good sample applicability.

Description

technical field [0001] The invention relates to a detection method for solanesol filter tip interception in mainstream smoke of cigarettes, and belongs to the field of tobacco chemistry. Background technique [0002] Solanesol is an unsaturated primary nonameric isoprenyl alcohol with the molecular formula C 45 h 74 O, a tetrasesquiterpene alcohol, has a unique chain-like isolated polyene structure. Solanesol is usually relatively stable, hardly soluble in water, and easily soluble in organic solvents. [0003] Solanesol widely exists in higher plants, mammals and microorganisms, and its content is prominent in tobacco leaves, potato leaves and mulberry leaves, especially in tobacco leaves, where the content of solanesol is as high as 0.3% to 3%. Solanesol in tobacco exists in two forms: free alcohol and compound form (mainly acetate, palmitate and linoleate). Studies have shown that when cigarettes are smoked, part of the solanesol in tobacco is cracked to produce fused...

AI Technical Summary

Problems solved by technology

However, at present, most of the researches on the analysis of solanesol are aimed at the analysis of solanesol in tobacco, cigarette mainstream smoke and environmental tobacco smoke, while there are few studies on the analysis methods of solanesol in filters.

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